• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2000 Fiscal Year Final Research Report Summary

Functional Analysis of the NMDA-recepter-APC-DLG-Complex.

Research Project

Project/Area Number 11470039
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field Pathological medical chemistry
Research InstitutionThe University of Tokyo

Principal Investigator

AKIYAMA Testu  Institute of Molecular and Cellular Biosciences, The University of Tokyo Professor, 分子細胞生物学研究所, 教授 (70150745)

Project Period (FY) 1999 – 2000
KeywordsNMDA receptor / Tumor suppressor / APC / PSD-95 / SPAL / synapse / post synaptic density / learning
Research Abstract

The synaptic protein PSD95/SAP90 interacts with ion channels such as the N-methyl-D-aspartate-receptor (NMDA-R) via its PDZ domain, and is involved in their clustering. Moreover, it interacts with signaling molecules and plays an important role in coupling NMDA-R to pathways that control synaptic plasticity and learning. We found that PSD-95 is associated with the adenomatous polyposis coli (APC) tumor suppressor protein, Rap1-specific GAP (SPAL), LGN and tyrosine kinase Fyn. Our findings raise the possibility that these molecules are involved in the regulation of NMDA-R clustering and/or NMDA-R-mediated signal transduction.

  • Research Products

    (40 results)

All Other

All Publications (40 results)

  • [Publications] F.Hamada: "Negative regulation of Wingless signaling by D-Axin, a Drosophila homologue of Axin."Science. 283. 1739-1742 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Yamochi: "Adenovirus-mediated high expression of BCL-6 in CV-1 cells induces apoptotic cell death accompanied by downregulation of BCL-2 and BCL-XL."Oncogene. 18. 487-494 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Tezuka: "PSD-95 promotes Fyn-mediated tyrosine phosphorylation of the N-methyl-D-aspartate receptor subunit NR2A."Proc.Natl.Acad.Sci.USA. 96. 435-440 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Senda: "Localization of MCC (mutated in colorectal cancer) in various tissues of mice and its involvement in cell differentiation."J Histochem Cytochem. 47. 1149-1158 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] C.R,Badal: "Localization of the GAP family SPAL in the Rat Esophagus and Heart."Med.Electron Microsc.. 32. 20-24 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Suzuki: "Tax oncoprotein of HTLV・1 binds to the human homologue of Drosophila dises large tumor suppressor protein, hDLG, and perturbs its function in cell growth control."Oncogene. 18. 5967-5972 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Takemura: "Nucleolar protein B23.1 binds to retinoblastoma protein and synergistically stimulates DNA polymerase alpha activity."J.Biochem.. 125. 904-909 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Ishidate: "The APC-hDLG Complex Negatively Regulates Cell Cycle Progression from the G0/G1 to SPhase."Oncogene. 19. 356-372 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Nakagawa: "EB3, a novel member of the EB1 family preferentially expressed in the central nervous system, binds to a CNS-specific APC homologue."Oncogene. 19. 210-216 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Nakagawa: "APCL, a central nervous system-specific homolog of adenomatous polyposis coli tumor suppressor, binds to p53-binding protein 2 and translocates it to the perinucleus."Cancer Res.. 60. 101-105 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Kawahara: "Downregulation of β-catenin by the Colorectal Tumor Suppressor APC Requires Association with Axin and b-catenin."J.Biol.Chem.. 275. 8369-8374 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Tago: "Inhibition of Wnt signaling by ICAT, a Novel β-catenin-inte racting Protein."Gense Dev.. 14. 1741-1749 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Akiyama: "Wnt/β-catenin signaling."Cytokine & Growth Factor Reviews. 11. 273-282 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Yanai: "The Colorectal Tumor Suppressor APC is Present in the NMDA-receptor-PSD-95 Complex in the Brain."Genes Cells. 5. 815-822 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Kawasaki: "Asef, a Link between the Tumor Suppressor APC and C-protein Signaling."Science. 289. 1194-1197 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Haraguchi: "The hDLG-associated protein DAP interacts with dynein light chain and neuronal nitric oxide synthase."Genes Cells. 5. 905-911 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Dobashi: "APC protein is required for initiation of neuronal differentiation in rat pheochromocytoma PC12 cells."Biochem.Biophys.Res.Commun.. 279. 685-691 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Senda: "Expression of APC in the mouse kidney and its colocalization with β-catenin and hDLG."J.Histochem.Cytochem.. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kohu,K.: "Localization of the Novel GAP Family Protein SPAL in Epithelial Cells."Acta Histochem.Cytochem.. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kakinuma,N.: "APC is Colocalized with β-Catenin and hDLG in the Henle's Loop of the Mouse Kidney."Acta Histochem.Cytochem.. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] F.Hamada, Y.Tomoyasu, Y.Takatsu, M.Nakamura, S.Nagai, A.Suzuki, F.Fujita, H.Shibuya, K.Toyoshima, N.Ueno & T.Akiyama: "Negative regulation of Wingless signaling by D-Axin, a Drosophila homologue of Axin."Science. 283. 1739-1742 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Yamochi, Y.Kaneita, T.Akiyama, S.Mori & M.Moriyama: "Adenovirus-mediated high expression of BCL-6 in CV-1 cells induces apoptotic cell death accompanied by downregulation of BCL-2 and BCL-XL."Oncogene. 18. 487-494 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Tezuka, H.Umemori, T.Akiyama, S.Nakanishi & T.Yamamoto: "PSD-95 promotes Fyn-mediated tyrosine phosphorylation of the N-methyl-D-aspartate receptor subunit NR2A."Proc.Natl.Acad.Sci.USA. 96. 435-440 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Senda, A.Matsumine, H.Yanai, T.Akiyama: "Localization of MCC (mutated in colorectal cancer) in various tissues of mice and its involvement in cell differentiation."J Histochem Cytochem. 47. 1149-1158 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] C.R,Badal, T.Kuroda, S.Mori, K.Kohu, T.Akiyama and T.Senda: "Localization of the GAP family SPAL in the Rat Esophagus and Heart."Med.Electron Microsc.. 32. 20-24 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Suzuki, Y.Ohsugi, M.Uchida-Toita, T.Akiyama and M.Yoshida: "Tax oncoprotein of HTLV-1 binds to the human homologue of Drosophila discs large tumor suppressor protein, hDLG, and perturbs its function in cell growth control."Oncogene. 18. 5967-5972 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Takemura, K.Sato, M.Nishio, T.Akiyama, H.Umekawa, & S.Yoshida: "Nucleolar protein B23.1 binds to retinoblastoma protein and synergistically stimulates DNA polymerase alpha activity."J.Biochem.. 125. 904-909 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Ishidale, A.Matsumine, K.Toyoshima, and T.Akiyama: "The APC-hDLG Complex Negatively Regulates Cell Cycle Progression from the G0/G1 to S Phase."Oncogene. 19. 365-372 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Nakagawa, K.Koyama, Y.Murata, M.Morito, T.Akiyama and Y.Nakamura: "EB3, a novel member of the EB1 family preferentially expressed in the central nervous system, binds to a CNS-specific APC homologue."Oncogene. 19. 210-216 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Nakagawa, K.Koyama, Y.Murata, M.Monden, T.Akiyama and Y.Nakamura: "APCL, a central nervous system-specific homolog of adenomatous polyposis coli tumor suppressor, binds to p53-binding protein 2 and translocates it to the perinucleus."Cancer Res.. 60. 101-105 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Kawahara, T.Morishita, T.Nakamura, F.Hamada, K.Toyoshima, and T.Akiyama: "Downregulation of b-catenin by the Colorectal Tumor Suppressor APC Requires Association with Axin and b-catenin."J.Biol.Chem.. 275. 8369-8374 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Tago, T.Nakamura, M.Nishita, J.Hyodo, S.Nagai, Y.Murata, S.Adachi, S.Ohwada, Y.Morishita, H.Shibuya and T.Akiyama: "Inhibition of Wnt signaling by ICAT, a Novel b-catenin-interacting Protein."Gense Dev.. 14. 1741-1749 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Akiyama: "Wnt/b-catenin signaling."Cytokine & Growth Factor Reviews. 11. 273-282 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Yanai, K.Satoh, A.Matsumine and T.Akiyama: "The Colorectal Tumor Suppressor APC is Present in the NMDA-receptor-PSD-95 Complex in the Brain."Genes Cells. 5. 815-822 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Kawasaki, T.Senda, T.Ishidate, R.Koyama, T.Morishita, Y.Iwayama, O.Higuchi, T.Akiyama: "Asef, a Link between the Tumor Suppressor APC and G-protein Signaling."Science. 289. 1194-1197 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Haraguchi, K.Satoh, H.Yanai, F.Hamada, M.Kawabuchi and T.Akiyama: "The hDLG-associated protein DAP interacts with dynein light chain and neuronal nitric oxide synthase."Genes Cells. 5. 905-911 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Dobashi, K.Katayama, M.Kawai, T.Akiyama and T.Kameya: "APC protein is required for initiation of neuronal differentiation in rat pheochromocytoma PC12 cells."Biochem.Biopys.Res.Commun.. 279. 685-691 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Senda, Y.Nishimura, H.Yanai and T.Akiyama: "Expression of APC in the mouse kidney and its colocalization with b-catenin and hDLG."J.Histochem.Cytochem.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kohu, K., Roy, B.C., Kuroda, T., Kakinuma, N., Akiyama, T.and Senda, T.: "Localization of the Novel GAP Family Protein SPAL in Epithelial Cells."Acta Histochem.Cytochem.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kakinuma, N., Nishimura, Y., Akiyama, T.and Senda, T.: "APC is Colocalized with β-Catenin and hDLG in the Henle's Loop of the Mouse Kidney."Acta Histochem.Cytochem.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2002-03-26  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi