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2001 Fiscal Year Final Research Report Summary

Molecular-pathological study on the apoptosis-regulating factors and signal transduction in human gastrointestinal carcinomas

Research Project

Project/Area Number 11470050
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Human pathology
Research InstitutionTottori University

Principal Investigator

ITO Hisao  Tottori University, Faculty of Medicine, Dep. of Pathology, Professor, 医学部, 教授 (60127610)

Project Period (FY) 1999 – 2001
Keywordsapoptosis / p53 gene / FAS / intratumoral microvessel density / signal transduction / Caspase / ARPP / minichromosome maintenance-2
Research Abstract

1) Apoptotic index of the tumor cells and intratumoral microvessel density (IMVD) showed a significant correlation in human esophageal, gastric and colonic carcinomas.
2) Adenovirus-mediated transfer of wild-type p53 gene resulted in apoptosis (MKN-1), cell arrest (MKN-45, 74 and KATO-III), and non-effectiveness (TMK-1 and OCUM-2M), regardless of p53 gene status. Upregulation of BAX might correlate with apoptosis of MKN-1 cells.
3) FAS and FAS-ligand variably expressed in human esophageal and gastric precancerous lesions and carcinomas, in which apoptosis occurs via FAS-dependent and -independent pathway. Upregulation of FAS-ligand correlated with the progression of the human gastrointestinal carcinomas. No aberration of FAS gene was confirmed in human gastrointestinal carcinomas. Signal transduction through FAS occurs via mitochondria pathway.
4) Gastrointestinal carcinomas variably expressed thymidine phosphorylase and/or cyclooxygenase-2(COX-2), both of which significantly caused increase of IMVD and decrease of apoptosis of the tumor cells.
5) A novel ankirin repeated protein ARPP, consisted 333 a.a. was cloned from the human esophageal carcinoma cell line, TE-1. ARPP expressed in normal skeletal and cardiac muscle, as well as various gastrointestinal carcinoma cell lines. ARPP might play a significant role to inhibit apoptosis.
6) DNA-replication regulating factor, minichromosome maintenance-2(MCM2) is a new, reliable marker for the rapid detection of proliferating cells in normal and neoplastic tissues.

  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] Endo K, Ito H, et al.: "Wild-type p53 gene transfer resulted in cell cycle arrest, but not apoptosis of newly established human malignant fibroushistiocytoma cell line"Int J Oncol. 15. 935-942 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsuura T, Ito H, et al.: "Thymidine phosphorylase expression is associated with both increase of intratumoral microvessels and decrease of apoptosis in human colorectal carcinomas"Cancer Res. 59. 5037-5040 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shomori K, Ito H, et al.: "Thymidine phosphorylase expression in human colorectal mucosa, adenoma and carcinoma : role of p53 expression"Pathol Int. 49. 491-499 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Doi R, Ito H, et al.: "Expression of p53 oncoprotein increases intratumoral microvessel fomation in human salivary gland carcinomas"J Oral Pathol Med. 28. 259-263 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tatebe S, Ito H, et al.: "Adenovirus-mediated transfer of wild-type p53 gene results in apoptosis or growth arrest in human cultured gastric carcinoma cells"Int J Oncol. 15. 229-235 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsura T, Ito H, et al.: "Hydrogen peroxide-induced apoptosis in HL-60 cells requires caspase-3 activation"Free Radic Res. 30. 73-83 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Goto A, Ito H, et al.: "Hyperthermia-induced apoptosis occurs both in a p53 gene-dependent and independent manner in three human gastric carcinoma cell lines"Oncol Rep. 6. 335-339 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsuura T, Ito H, et al.: "Preoperative treatment with tegafur suppositories enhances apoptosis and reduces the intratumoral microvessel density of human coiorectal carcinoma"Cancer. 88. 1007-1015 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Osaki M, Ito H, et al.: "Thymidine phosphorylase expression results in a decrease in apoptosis and increase in intratumoral microvessel density in human gastric carcinomas"Virchows Arch. 437. 31-36 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sakatani T, Ito H, et al.: "Expressions of thymidine phosphorylase (dThdPase) and vascular endothelial growth factor on angiooenesis in intestinal-type gastric carcinoma"Oncol Rep. 7. 831-836 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Endo K, Ito H, et al.: "Wild-type p53 gene transfection in human cultured sarcomas : effect of CDDP"Oncol Rep. 8. 637-642 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Osaki M, Ito H, et al.: "Expression of Fas and Fas ligand in human gastric adenomas and intestinal-type carcinomas correlation with proliferation and apoptosis"Gastric Cancer. 4. 198-205 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Moriyama M, Ito H, et al.: "Identification of a novel human ankyrin-repeated protein homologous to CARP"Biochem Biophys Res Commun. 20. 715-723 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okamoto E, Ito H, et al.: "Thyrmidine phosphorylase expression causes both the increase of intratumoral microvessels and decrease of apoptosis in human esophageal carcinomas"Pathol Int. 51. 158-164 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Endo K, Ito H. et al: "Wild-type p53 gene transfer resulted in cell cycle arrest, but not apoptosis of newly established human malignant fibroushistiocytoma cell line"Int J Oncol.. 15 (5). 935-942 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsuura T, Ito H. et al.: "Thymidine phosphorylase expression is associated with both increase of intratumoral microvessels and decrease of apoptosis in human colorectal carcinomas"Cancer Res.. 59 (19). 5037-5040 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shomori K, Ito H. et al.: "Thymidine phosphorylase expression in human colorectal mucosa, adenoma and carcinoma : role of p53 expression"Pathol Int.. 49 (6). 491-499 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Doi R, Ito H. et al.: "Expression of p53 oncoprotein increases intratumoral microvessel formation in human salivary gland carcinomas"J Oral Pathol Med.. 28 (6). 259-263 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tatebe S, Ito H. et al.: "Adenovirus-mediated transfer of wild-type p53 gene results in apoptosis or growth arrest in human cultured gastric carcinoma cells"Int J Oncol.. 15 (2). 229-235 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsuura T, Ito H. et al.: "Hydrogen peroxide-induced apoptosis in HL-60 cells requires caspase-3 activation"Free Radic Res.. 30 (1). 73-83 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Goto A, Ito H. et al.: "Hyperthermia-induced apoptosis occurs both in a p53 gene-dependent and -independent manner in three human gastric carcinoma cell lines"Oncol Rep.. 6 (2). 335-339 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsuura T, Ito H. et al.: "Preoperative treatment with tegafur suppositories enhances apoptosis and reduces the intratumoral microvessel density of human colorectal carcinoma"Cancer. 88 (5). 1007-1015 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Osaki M, Ito H. et al.: "Thymidine phosphorylase expression results in a decrease in apoptosis and increase in intratumoral microvessel density in human gastric carcinomas"Virchows Arch.. 437 (1). 31-36 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sakatani T, Ito H. et al.: "Expressions of thymidine phosphorylase (dThdPase) and vascular endothelial growth factor on angiogenesis in intestinal-type gastric carcinoma"Oncol Rep.. 7 (4). 831-836 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Endo K, Ito H. et al.: "Wild-type p53 gene transfection in human cultured sarcomas : effect of CDDP"Oncol. Rep.. 8 (3). 637-642 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Osaki M, Ito H. et al.: "Expression of Fas and Fas ligand in human gastric adenomas and intestinal-type carcinomas : correlation with proliferation and apoptosis"Gastric Cancer. 4 (4). 198-205 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Moriyama M, Ito H. et al.: "Identification of a novel human ankyrin-repeated protein homologous to CARP"Biochem Biophys Res Commun. 20 ; 285 (3). 715-723 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okamoto E, Ito H. et al.: "Thymidine phosphorylase expression causes both the increase of intratumoral microvessels and decrease of apoptosis in human esophageal carcinomas"Pathol Int.. 51 (3). 158-64 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kodani I, Ito H. et al.: "Expression of minochromosome maintenance 2 (MCM2), Ki-67, and cell-cycle-related molecules, and apoptosis in the normal-dysplasia-carcinoma sequence of the oral mucosa"Pathobiology. 69 (3). 150-158 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kase S, Ito H. et al: "Expression of Fas and Fas ligand in esophageal tissue mucosa and carcinomas"Int J Oncol.. 20 (2). 291-297 (2002)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2003-09-17  

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