2001 Fiscal Year Final Research Report Summary
Elucidation of the mechanism of lineage commitment and differentiation in T cell development
| Project/Area Number |
11470086
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Immunology
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
KATSURA Yoshimoto Institute for Frontier Medical Science, Kyoto Univ., Professor, 再生医科学研究所, 教授 (90027095)
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| Co-Investigator(Kenkyū-buntansha) |
FUJIMOTO Shinji Institute for Frontier Medical Science, Kyoto Univ., Assist. Prof., 再生医科学研究所, 助手 (60199370)
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| Project Period (FY) |
1999 – 2001
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| Keywords | T cell / NK cell / differentiation / thymus / progenitor |
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| Research Abstract |
In our previous studies, we have shown that T cell progenitors (p-T) are produced in fetal liver from the hematopoietic stem cell (HSC) through the stage of p-MT that is capable of generating myeloid and T cells. It was unclear, however, whether the p-MT are directly produced from the HSC or through an undetermined intermediate stage. In the present study, we newly developed the MLP<METB> that is assay capable of determining the developmental potential of individual progenitors towards myeloid, erythroid, T and B cell lineages. With this assay, we identified the p-MTB, the common progenitor for p-MT and PMB. P-METB (HSC) and p-ME were also identified. We have previously shown that prethymic p-T as well as the earliest thymic p-T retain NK potential. In the present study, we showed that these p-T/NK also retain DC potential. The developmental potential of p-T/NK/DC were found to be sequentially restricted to T cell lineages in the thymus during the progression of stages from CD44+CD25" to CD44 CD25+. By introducing the Id2 gene into the p-T/NK/DC with a retroviral vector, generation of αβT cells but not γδT cells was completely arrested.
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