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2001 Fiscal Year Final Research Report Summary

Establishment of Langerhans cell-cell line and its spplication for treatment

Research Project

Project/Area Number 11470179
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Dermatology
Research InstitutionThe University of Tokyo

Principal Investigator

TAMAKI Kunihiko  Faculty of Medicine, Professor, 医学部附属病院, 教授 (30010432)

Co-Investigator(Kenkyū-buntansha) NAKAMURA Koichiro  Faculty of Medicine, Lecturer, 医学部附属病院, 講師 (60175502)
AOSHIMA Akihiko  Faculty of Medicine, Lecturer, 医学部附属病院, 講師 (50202601)
Project Period (FY) 1999 – 2001
KeywordsLangerhans cells / cytokine production / esiticesfeird / CD23 / Prion / IL-12 / Th_1 / TGF-β
Research Abstract

We succeeded in getting purified (over 95%) mouse Langerhans cello (LC) and analyzed their characteristics. Although we have not yet succeeded in establishment of LC-all line , we could get the following results.
(1) Using purified LC we analyzed the maturing mechanism during LC culture, (2) We also found the effect of GM-CSF, M-CSF, TNF-α, corticasteroid, cyclosporine A and FK506 on LC, (3) The effect of GM-CSF and TGF-β on IL-12 production by LC was studied. (4) CD23 expression by mouse LC and its modulation by cytokines was studied, and (4) Prion related protein expression by LC was found. The induction by TGF-βtreated LC is now under in imoestigation.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Nagaoka Y et al.: "Identification and characterization of CD23 on mouse Langerhans cells"J Invest Dermatol. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sugaya M et al.: "Expression of cellular prion-related protein by murine Langerhans cells and Reractinocytes"J Derm Sci. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tamaki K et al.: "The role of lymphocytes in healthy and eczematous skin"Curr Opinion Allergy Clin Immunol. 1. 455-460 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tada Y et al.: "TGF-β upregulates IL-12 production by mouse Langerhans cells"Eur J Immunol. 31. 294-300 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tada Y et al.: "Granulocyte/macrophage colony-stimulating factor inhibits IL-12 production of mouse Langerhans cells"J Immunol. 164. 5113-5119 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Salgado CG et al.: "Differential effects of cytokines and immunosuppressive drugs on CD40, B7-1, and B7-2 expression on purified epidermal Langerhans cells"J Invest Dermatol. 113. 1021-1027 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nagaoka Y et al: "Identification and characterization of CD23 on mouse langerbeno cells"J Inaest Dermatol. in press.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sugaya M et al: "Expression of celluter prion-related protein by murine langerhans cells and keratinoytes"J Derm Sei. in press.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tamaki K et al: "The role of lymphocytes inhealthy and eyematous skin"Curr Opinion Allergy Clin Immunol. 1. 455-460 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tada Y et al: "TGF-β upregulaters IL-12 production by mouse langerhans cells"Eur J Immunol. 31. 294-300 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tada Y et al: "GM/CSF inhibits IL-12 production by mouse Langerhams cells"J Immunol. 164. 5113-5119 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Salgada CG etal: "Diffrential effect of cytokines and immunosuppressiot drugs on CD40, B7-1, and B7-2 expression on purfied spideunal langerhans cells"J Inoest Dernitol. 113. 1021-1027 (1999)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2004-04-14  

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