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2001 Fiscal Year Final Research Report Summary

Systematic Isolation of Novel Genes Involved in Regulation of Growth and Differentiation of Slain Cells and Their Relevance to Human Diseases.

Research Project

Project/Area Number 11470180
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Dermatology
Research InstitutionOKAYAMA UNIVERSITY (2001)
Toyama Medical and Pharmaceutical University (1999-2000)

Principal Investigator

HUH Nam-ho  Graduate School of Med. & Dent., Prof., 大学院・医歯学総合研究科, 教授 (70173573)

Co-Investigator(Kenkyū-buntansha) MOROHASHI Masaaki  Toyama Med. Pharm. Univ., Facult. Med., Prof., 医学部, 教授 (50018719)
TAKAISHI Mikiro  Graduate School of Med. & Dent., Res. Assist., 大学院・医歯学総合研究科, 助手 (10303223)
MASAHIRO Miyazaki  Graduate School of Med. & Dent., Assoc. Prof., 大学院・医歯学総合研究科, 助教授 (90116509)
Project Period (FY) 1999 – 2001
Keywordsskin / RNA differential display / gene / differentiation / filaggrin / actin / keratinization / Ca-binding
Research Abstract

[Objective] In aiming of better understanding of the mechanisms underlying in major human skin diseases and establishing fundamental therapeutic measures, we planned to systematically isolate genes involved in the regulation of growth and differentiation of skin cells.
[Methods] RNA differential display was applied to developing mouse skin (12.5 dpc to 16.5 dpc) in which more differentiated epidermal layers are added to existing layer(s) with the progress of development.
[Results] We have isolated 6 novel genes, expression levels of which increased during the observation period, and 2 novel genes, which showed a decreasing expression profile during the period. Representative genes are as follows. 2) Hornerin : Hornerin protein has a Ca-binding EF-hand domain in the N-terminal side and repetitive structures in the remaining part. We showed that hornerin protein was co-localized with profilaggrin in keratohyalin granules of granular cells in the skin, tongue, esophagus, and forestomach. When normal human keratinocytes were induced to differentiate by high Ca, expression of hornerin was increased concomitantly with profilaggrin. These results indicate that hornerin is structurally and functionally similar to frofilaggrin and involved in keratinization like profilaggrin. 3) Calmin : Calmin protein has two actin-binding calponin-homology domains at the N-terminus and a transmembrane domain at the C-terminus. It is expressed in the skin, testis, brain (hippocampus, hypothalamus, and Purkinje cells), liver and kidney. The expression levels increase in association with developmental maturation of those organs. 4) tpis : tpis is characterized by possessing tetratriopeptide repeats and presumably involved in differentiation of epidermal keratinocytes. 5) Other related results : Based on these results and techniques, we analyzed developing mouse placenta and succeeded in isolating a number of novel cancer-related gene.

  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Takaishi, M.: "A tetratricopeptide repeat-containing protein gene, tpis, whose expression is induced with differentiation of spermatogenic cells."Biochem.Biophys.Res.Commun.. 264(1). 81-85 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakajima, A.: "Cathepsin-6, a novel cysteine proteinase showing homology with and co-localized expression with cathepsin J/P in the labyrinthine layer of mouse placenta."Biochem.J.. 349. 689-692 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kataoka, K.: "Screening for genes involved in tissue invasion based on placenta formation and cancer cell lines with low and high metastatic potential."Cancer Lett.. 163(2). 213-219 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishisaki, Z.: "Calmin, a protein with calponin homology and transmembrane domains expressed in maturing spermatogenic cells."Genomics. 74(2). 172-179 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamago, G.: "Suppression of hair follicle development inhibits induction of sonic hedgehog, patched, and patched-2 in hair germs in mice."Arch.Dermatol.Res.. 293(9). 435-441 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Makino, T.: "Hornerin, a novel profilaggrin-like protein and differentiation-specific marker isolated from mouse skin."J.Biol.Chem.. 276(50). 47445-47452 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 許 南浩(分担執筆, 共編): "形態形成実験法「分子生物学のための新培養細胞実験法」"羊土社. 316 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takaishi, M. et al.: "At tetratricopeptide repeat-containing protein gene, t pis, whose expression is induced with differentiation of spermatoganic cells."Biochem.Biophys.Res.Commun.. 264(1). 81-85 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kataoka et al.: "A carcinoembryonic antigen family cDNA from mouse placenta encoding a protein with a rare domain composition."Placenta. 21(7). 610-614 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakajima, A. et al.: "Cathepsin-6, a novel cysteine proteinase showing homology with and co-localized expression with cat hepsin J/ P in the labyrinthine layer of mouse placenta."Biochem.J.. 349. 689-692 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kataoka, K. et al.: "Screening for genes involved In tissue invasion based on placenta format ion and cancer cell lines with low and high metastatic potential."Cancer Lett.. 163(2). 213-219 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishisaki, Z. et al.: "Calmin, a protein with calponin homology and transmembrane domains expressed in maturing spermatogenic cells."Genomics. 74(2). 172-179 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamago, G. et al.: "Suppression of hair follicle development inhibits induction of sonic hedgehog, patched, and patched-2 in hair germs in mice."Arch.Dermatol.Res.. 293(9). 435-441 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Niino, Y.S.et al.: "PKCII, a new isoform of protein kinase C specifically expressed in the seminiferous tubules of mouse test is."J.Biol.Chem.. 276(39). 36711-36717 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Makino, T. et al.: "Hornerin, a novel profilaggrin-like protein and differentiation-specific marker isolated from mouse skin."J.Biol.Chem.. 276(50). 47445-47452 (2001)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2005-04-19  

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