2001 Fiscal Year Annual Research Report

皮膚樹状細胞の活性化・遊走シグナルの解析と修飾、およびその治療への応用

Research Project

Project/Area Number	11470183
Research Institution	KYUSHU UNIVERSITY
Principal Investigator	古江増隆九州大学, 大学院・医学研究院, 教授 (70134583)
Co-Investigator(Kenkyū-buntansha)	寺尾浩九州大学, 医学部・附属病院, 助手 (80274447)
Keywords	樹状細胞 / ランゲルハンス細胞 / サイトカイン / ケモカイン / 乾癬 / 真菌乾癬 / 抗酸化剤
Research Abstract	皮膚樹状細胞(ランゲルハンス細胞)の活性化と遊走シグナルは密に関連して合目的に制御されていると考えられる。11年度はハプテン外用後に生ずるランゲルハンス細胞の活性化の病態(J Invest Dermatol(Synp Proc)4:164,1999)、ランゲルハンス細胞の真皮から表皮内への移入におけるIL6とTNF-αの役割(J Dermatol Sci 19:166,1999)について明らかにした。12年度は、表皮内有棘細胞癌であるボーエン病におけるCD1a陽性樹状細胞と表皮内CD3,CD4,CD68各陽性細胞数との動態と腫瘍細胞との関連を明らかにした(Brit J Dermatol 143:1211,2000)。また抗酸化剤の一つであるCX-659Sがマウスならびにモルモットの接触皮膚炎のいくつかのモデルを抑制すること、局所のIL-1bやTNF-a産生を抑制することを明らかにした(Int. Arch.Anergy Immnol 123:341,2000)。現在、表皮細胞のGM-CSF産生とランゲルハンス細胞の活性化に対する抑制効果を検討している。さらに黒色腫におけるマクロファージの動態とTNF-a、IL-8、VEGFの産生の関連性を明らかにした(Int. J Cancer85,182,2000,Jap J Cancer Res91:906,2000)。13年度は、樹状細胞の場を形成する表皮細胞のケモカイン・サイトカインの動態とIL-13 receptorの局在を観察し(J Dermatol Sci 25:229-235,2001,Cytokine 13:75-84,2001,J Dermatol Sci, in press)、真菌感染症や乾癬におけるIFN-gやIL-8産生細胞の局在を明らかにした(Eur J Dermatol 11:105, 2001,J Demmtol Sci 26:119,2001,Eur J Demmtol, in press, Dermatology, in press)。また腫瘍消腿や真菌性肉芽腫、皮膚リンパ濾胞形成における樹状細胞の関与を指摘した(Dendriti Cells 11:13,2001,Clin Exp Dermatol 26:705,2001,Eur J Dermatol,11:527,2001)。

Research Products

(28 results)

All Other

All Publications (28 results)

[Publications] M Furue, H Duan, H Uchi, et al.: "Multiple spontaneous regression of seborrheic keratosis associated with nasal carcinoma"Clin Exp Dermatol. (in press).
[Publications] Koga T, Duan H, Urabe K, Furue M: "Immunohistochemical localization of activated and mature CD83+ dendritic cells in granulomas of sprotrichosis"Eur J Dermatol. (in press).
[Publications] Koga T, Duan H, Urabe K, Furue M: "In situ localization of IFN-γ positive cells in psoriatic lesional epidermis"Eur J Dermatol. (in press).
[Publications] Koga T, Duan H, Urabe K, Furue M: "In situ localization of CD83-positive dendritic cells in psoriatic lesions"Dermatology. (in press).
[Publications] Kohda F, Koga T, Uchi H, Urabe K, Furue M: "Histamine-induced IL-6 and IL-8 production are differentially modulated by IFN-γ and IL-4 in human keratinocytes"J Dermatol Sci. (in press).
[Publications] Wakugawa M, Nakamura K, Tamaki K, Furue M et al.: "Expression of CC chemokine receptor on human keratinocytes in vivo and in vitro -upregulation by RANTES-"J Dermatol Sci. 25. 229-235 (2001)
[Publications] Koga T, Duan H, Urabe K, Furue M: "Immunohistochemical detection of interferon-γ -producing cells in dermatophytosis"Eur J Dermatol. 11. 105-107 (2001)
[Publications] Duan H, Koga T, Kohda F, Hara H, Urabe K, Furue M: "Interleukin-8-positive neutrophils in psoriasis"J Dermatol Sci. 26. 119-124 (2001)
[Publications] Akikawa M, Yu B, Umeshita-Suyama R, Furue M et al.: "Localization of interleukin 13 receptor in non-heamatopoietic cells"Cytokine. 13. 75-84 (2001)
[Publications] Yoshida Y, Duan H, Koga T, Furue M: "Immunohistochemical study on cutaneous lymphoid follicles in lymphadenosis benigna cutis, angiolymphoid hyperplasia with eosinophilia, and Kimura's disease in comparison with normal human lymph nodes"Dendriti Cells. 11. 13-16 (2001)
[Publications] Juichiro Nakayama, Hiroshi Terao, Tetsuya Koga, Masutaka Furue: "Induction of CD54and CD58 expression in cultured human endothelial cells by β-interferon with or without hyperthermia in vitro"Journal of Dermatological Science. 26. 19-24 (2001)
[Publications] Yoshinori Ohnishi, Katsuya Hirano, Junji Nishimura, Masutaka Furue, Hideo Kanaide: "Inhibitory effects of brefeldin A, a membrane transport blocker, on the bradykinin-induced hyperpolarization-mediated relaxation in the porcine coronary artery"British Journal of Pharmacology. 134. 168-178 (2001)
[Publications] H Duan, T Koga, T Masuda, H Kiryu, M Furue et al.: "CD1a-H, CD3+, CD4+, CDS+, CD68+ and CLA+ cells in Bowen's disease"Brit J Dermatol. 143. 1211-1216 (2000)
[Publications] Goto Y, Inoue Y, Tsuchida M, Isobe M, Ueno T, Uchi H, Furue M et al.: "Suppressive Effect of Topically Applied CX-659S, a Novel Diaminouracil Derivative, on the Contact Hypersensitivity Reaction in Various Animal Models"Int.Arch.Allergy Immunol. 123. 341-348 (2000)
[Publications] Hitoe TORISU, Mayumi ONO, Hiromaro KIRYU, Masutaka FURUE: "Macrophage infiltration correlates with tumor stage and angiogenesis in human malignant melanoma : Possible involvement of TNF α and IL-1 α"Int J Cancer. 85. 182-188 (2000)
[Publications] Hitoe TORISU-ITAKURA, Masutaka FURUE et al.: "Co-expression of tymidine phosphorylase and heme oxygenase-1 in macrophages in human malignant vertical growth melanomas"Jap J Cancer Res. 91. 906-910 (2000)
[Publications] Izumi Horiuchi, Yuji Kawano, Kenji Yamasaki, Motozumi minohara, Masutaka Furue et al.: "Th1 dominance in HAM/TSP and the optico-spinal form of multiple sclerosis versus Th2 dominance in mite antigen-specific IgE myelitis"J Neurol Sci. 172. 17-24 (2000)
[Publications] Wakugawa M, Nakamura K, Hino H, Furue M et al.: "Elevated levels of eotaxin and interleukin-5 in blister fluid of bullous pemphigoid : correlation with eosinophilia"Brit J Dermatol. 143. 112-116 (2000)
[Publications] Yasunobu Abe, Koichiro Muta, Koichi Oshima, Masutaka Furue, et al.: "Subcutaneous panniculitis by Epstein-Barr virus-infected natural killer (NK) cell proliferation terminating in aggressive subcutaneous NK cell lymphma"Am J Hematol. 64. 221-225 (2000)
[Publications] Araki M, Imafuku S, Furue M et al.: "Activation pattern of Langerhans cells in the afferent and efferent phases of contact hypersensitivity"J Invest Dermatol (Synp Proc). 4. 164-168 (1999)
[Publications] Saitoh A, Yasaka N, Osada A, Nakamura K, Furue M et al.: "Migration of Langerhans cells in an in vitro organ culture system : IL-6 and TNF-a are partially responsible for migration Into the epidermis"J Dermatol Sci. 19. 166-174 (1999)
[Publications] Duan H, Imafuku S, Sueishi K, Hori Y, Furue M et al.: "The proliferative properties of tumor cells differentially correlate with the host immune responses in anogenital Bowen's disease"J Dermatol Sci. 20. 53-62 (1999)
[Publications] Furue M, Koga T, Yamashita N: "Soluble E-selectin and eosinophil cationic protein are distinct serum markers that differentially represent clinical features of atopic dermatitis"Brit J Dermatol. 140. 67-72 (1999)
[Publications] Kira J, Kawano Y, Furue M et al.: "Clinical, immunological and MRL features of myelitis with atopic dermatitis (atopic myelitis)"J Neurol Sci. 162. 56-61 (1999)
[Publications] Toshitani A, Yoshinaga T, Furue M: "Reduced amount of secretory component of IgA secretion in tears of patients with atopic dermatitis"J Dermatol Sci. 19. 134-138 (1999)
[Publications] Nakayarna J, Kiryu H, Urabe K, Matsuo S, Koga T, Furue M: "Vitamin D3 analogues improve cafe-au lait spots in patients with von Recklinghausen's disease : experimental and clinical studies"Eur J Dermatol. 9. 202-206 (1999)
[Publications] Jun Matsunaga, Miwako Dakeishi-Hara, Muneo Tanita, Kanako Kikuchi, Masutaka Furue et al.: "A splicing mutation of the tyrosinase gene causes yellow oculocutaneous albinism in a Japanese patient with a pigmented phenotype"Dermatology. 199. 124-129 (1999)
[Publications] Katsunori Fujii, Toshiyuki Miyashita, Jun-ichi Takahashi, Katsuo Sugita, Masutaka Furue: "Gamma-irradiarion deregulates cell cycle control and apoptosis in nevoid basal cell carcinoma syndrome-derived cells"Jpn J Cancer Res. 90. 1351-1357 (1999)

2001 Fiscal Year Annual Research Report

皮膚樹状細胞の活性化・遊走シグナルの解析と修飾、およびその治療への応用

Principal Investigator

古江 増隆 九州大学, 大学院・医学研究院, 教授 (70134583)

Research Products

[Publications] M Furue, H Duan, H Uchi, et al.: "Multiple spontaneous regression of seborrheic keratosis associated with nasal carcinoma"Clin Exp Dermatol. (in press).

[Publications] Koga T, Duan H, Urabe K, Furue M: "Immunohistochemical localization of activated and mature CD83+ dendritic cells in granulomas of sprotrichosis"Eur J Dermatol. (in press).

[Publications] Koga T, Duan H, Urabe K, Furue M: "In situ localization of IFN-γ positive cells in psoriatic lesional epidermis"Eur J Dermatol. (in press).

[Publications] Koga T, Duan H, Urabe K, Furue M: "In situ localization of CD83-positive dendritic cells in psoriatic lesions"Dermatology. (in press).

[Publications] Kohda F, Koga T, Uchi H, Urabe K, Furue M: "Histamine-induced IL-6 and IL-8 production are differentially modulated by IFN-γ and IL-4 in human keratinocytes"J Dermatol Sci. (in press).

[Publications] Wakugawa M, Nakamura K, Tamaki K, Furue M et al.: "Expression of CC chemokine receptor on human keratinocytes in vivo and in vitro -upregulation by RANTES-"J Dermatol Sci. 25. 229-235 (2001)

[Publications] Koga T, Duan H, Urabe K, Furue M: "Immunohistochemical detection of interferon-γ -producing cells in dermatophytosis"Eur J Dermatol. 11. 105-107 (2001)

[Publications] Duan H, Koga T, Kohda F, Hara H, Urabe K, Furue M: "Interleukin-8-positive neutrophils in psoriasis"J Dermatol Sci. 26. 119-124 (2001)

[Publications] Akikawa M, Yu B, Umeshita-Suyama R, Furue M et al.: "Localization of interleukin 13 receptor in non-heamatopoietic cells"Cytokine. 13. 75-84 (2001)

[Publications] Yoshida Y, Duan H, Koga T, Furue M: "Immunohistochemical study on cutaneous lymphoid follicles in lymphadenosis benigna cutis, angiolymphoid hyperplasia with eosinophilia, and Kimura's disease in comparison with normal human lymph nodes"Dendriti Cells. 11. 13-16 (2001)

[Publications] Juichiro Nakayama, Hiroshi Terao, Tetsuya Koga, Masutaka Furue: "Induction of CD54and CD58 expression in cultured human endothelial cells by β-interferon with or without hyperthermia in vitro"Journal of Dermatological Science. 26. 19-24 (2001)

[Publications] H Duan, T Koga, T Masuda, H Kiryu, M Furue et al.: "CD1a-H, CD3+, CD4+, CDS+, CD68+ and CLA+ cells in Bowen's disease"Brit J Dermatol. 143. 1211-1216 (2000)

[Publications] Goto Y, Inoue Y, Tsuchida M, Isobe M, Ueno T, Uchi H, Furue M et al.: "Suppressive Effect of Topically Applied CX-659S, a Novel Diaminouracil Derivative, on the Contact Hypersensitivity Reaction in Various Animal Models"Int.Arch.Allergy Immunol. 123. 341-348 (2000)

[Publications] Hitoe TORISU, Mayumi ONO, Hiromaro KIRYU, Masutaka FURUE: "Macrophage infiltration correlates with tumor stage and angiogenesis in human malignant melanoma : Possible involvement of TNF α and IL-1 α"Int J Cancer. 85. 182-188 (2000)

[Publications] Hitoe TORISU-ITAKURA, Masutaka FURUE et al.: "Co-expression of tymidine phosphorylase and heme oxygenase-1 in macrophages in human malignant vertical growth melanomas"Jap J Cancer Res. 91. 906-910 (2000)

[Publications] Izumi Horiuchi, Yuji Kawano, Kenji Yamasaki, Motozumi minohara, Masutaka Furue et al.: "Th1 dominance in HAM/TSP and the optico-spinal form of multiple sclerosis versus Th2 dominance in mite antigen-specific IgE myelitis"J Neurol Sci. 172. 17-24 (2000)

[Publications] Wakugawa M, Nakamura K, Hino H, Furue M et al.: "Elevated levels of eotaxin and interleukin-5 in blister fluid of bullous pemphigoid : correlation with eosinophilia"Brit J Dermatol. 143. 112-116 (2000)

[Publications] Yasunobu Abe, Koichiro Muta, Koichi Oshima, Masutaka Furue, et al.: "Subcutaneous panniculitis by Epstein-Barr virus-infected natural killer (NK) cell proliferation terminating in aggressive subcutaneous NK cell lymphma"Am J Hematol. 64. 221-225 (2000)

[Publications] Araki M, Imafuku S, Furue M et al.: "Activation pattern of Langerhans cells in the afferent and efferent phases of contact hypersensitivity"J Invest Dermatol (Synp Proc). 4. 164-168 (1999)

[Publications] Saitoh A, Yasaka N, Osada A, Nakamura K, Furue M et al.: "Migration of Langerhans cells in an in vitro organ culture system : IL-6 and TNF-a are partially responsible for migration Into the epidermis"J Dermatol Sci. 19. 166-174 (1999)

[Publications] Duan H, Imafuku S, Sueishi K, Hori Y, Furue M et al.: "The proliferative properties of tumor cells differentially correlate with the host immune responses in anogenital Bowen's disease"J Dermatol Sci. 20. 53-62 (1999)

[Publications] Furue M, Koga T, Yamashita N: "Soluble E-selectin and eosinophil cationic protein are distinct serum markers that differentially represent clinical features of atopic dermatitis"Brit J Dermatol. 140. 67-72 (1999)

[Publications] Kira J, Kawano Y, Furue M et al.: "Clinical, immunological and MRL features of myelitis with atopic dermatitis (atopic myelitis)"J Neurol Sci. 162. 56-61 (1999)

[Publications] Toshitani A, Yoshinaga T, Furue M: "Reduced amount of secretory component of IgA secretion in tears of patients with atopic dermatitis"J Dermatol Sci. 19. 134-138 (1999)

[Publications] Nakayarna J, Kiryu H, Urabe K, Matsuo S, Koga T, Furue M: "Vitamin D3 analogues improve cafe-au lait spots in patients with von Recklinghausen's disease : experimental and clinical studies"Eur J Dermatol. 9. 202-206 (1999)

[Publications] Jun Matsunaga, Miwako Dakeishi-Hara, Muneo Tanita, Kanako Kikuchi, Masutaka Furue et al.: "A splicing mutation of the tyrosinase gene causes yellow oculocutaneous albinism in a Japanese patient with a pigmented phenotype"Dermatology. 199. 124-129 (1999)

[Publications] Katsunori Fujii, Toshiyuki Miyashita, Jun-ichi Takahashi, Katsuo Sugita, Masutaka Furue: "Gamma-irradiarion deregulates cell cycle control and apoptosis in nevoid basal cell carcinoma syndrome-derived cells"Jpn J Cancer Res. 90. 1351-1357 (1999)

古江増隆九州大学, 大学院・医学研究院, 教授 (70134583)