2000 Fiscal Year Final Research Report Summary
Mechanisms by which fucosyltransferases regulate epidermotropic migration of T cels
| Project/Area Number |
11470184
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Kyorin University |
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Principal Investigator |
SHIOHARA Tetsuo Kyorin University School of Medicine, Department of Dermatology, Professor, 医学部, 教授 (10118953)
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| Co-Investigator(Kenkyū-buntansha) |
HAYAKAWA Jun Kyorin University School of Medicine, Department of Dermatology, Instructor, 医学部, 助手 (30255393)
MIZUKAWA Yoshiko Kyorin University School of Medicine, Department of Dermatology, Instructor, 医学部, 助手 (50301479)
TERAKI Yuichi Kyorin University School of Medicine, Department of Dermatology, Assistant professor, 医学部, 講師 (10188667)
NARIMATSU Hisashi Soka University, Institute of Life Science, Professor, 生命研, 教授 (40129581)
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| Project Period (FY) |
1999 – 2000
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| Keywords | Fucosyltransferase VII / CLA / Th1 cells / Immunohistochemistry / CD4^+T cells / CD8^+T cells |
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| Research Abstract |
In this study, we for the first time developed a new mAb specific for Fuc-TVII that provides a means for the reliable identification of the enzyme in preparations of mixed cell populations or in tissues by immunohistochemistry. Using this mAb to Fuc-TVII, we found that in peripheral blood mononuclear cells (PBMC) and the purified CD4^+ and CD8^+ populations, unlike cell lines, the expression of Fuc-TVII in the cytoplasm was not necessarily associated with the surface expression of the CLA epitope : some cells expressed high levels of surface CLA but failed to express cytoplasmic Fuc-TVII and vice versa. Nevertheless, the percentages of Fuc-TVII-expressing CD4^+ or CD8^+ cells were close but not identical to those of CLA-expressing cells. According to the expression pattern of CLA and Fuc-TVII, the CD4^+ and CD8^+T cells consisted of three populations : Fuc-TVII^+CLA^+, Fuc-TVII^-CLA^+, and Fuc-TVII^+CLA^-. Our kinetics studies after in vitro stimulation with anti-CD3 mAb showed that the Fuc-TVII^-CLA^+ subset represents early activated cells that eventually differentiate into the Fuc-TVII^+CLA^+ subset and subsequently the Fuc-TVII^-CLA^+ subset. The capability of CD4^+ T cells to bind E-selectin was detected in the Fuc-TVII^+CLA^- and Fuc-TVII^+CLA^+ subset, but not the Fuc-TVII^- CLA^+. The Fuc-TVII^- CLA^+ subset was observed in increased number in PBMC coincident with resolution of the immune activation in various inflammatory settings.
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