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2000 Fiscal Year Final Research Report Summary

Mechanisms by which fucosyltransferases regulate epidermotropic migration of T cels

Research Project

Project/Area Number 11470184
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field Dermatology
Research InstitutionKyorin University

Principal Investigator

SHIOHARA Tetsuo  Kyorin University School of Medicine, Department of Dermatology, Professor, 医学部, 教授 (10118953)

Co-Investigator(Kenkyū-buntansha) HAYAKAWA Jun  Kyorin University School of Medicine, Department of Dermatology, Instructor, 医学部, 助手 (30255393)
MIZUKAWA Yoshiko  Kyorin University School of Medicine, Department of Dermatology, Instructor, 医学部, 助手 (50301479)
TERAKI Yuichi  Kyorin University School of Medicine, Department of Dermatology, Assistant professor, 医学部, 講師 (10188667)
NARIMATSU Hisashi  Soka University, Institute of Life Science, Professor, 生命研, 教授 (40129581)
Project Period (FY) 1999 – 2000
KeywordsFucosyltransferase VII / CLA / Th1 cells / Immunohistochemistry / CD4^+T cells / CD8^+T cells
Research Abstract

In this study, we for the first time developed a new mAb specific for Fuc-TVII that provides a means for the reliable identification of the enzyme in preparations of mixed cell populations or in tissues by immunohistochemistry. Using this mAb to Fuc-TVII, we found that in peripheral blood mononuclear cells (PBMC) and the purified CD4^+ and CD8^+ populations, unlike cell lines, the expression of Fuc-TVII in the cytoplasm was not necessarily associated with the surface expression of the CLA epitope : some cells expressed high levels of surface CLA but failed to express cytoplasmic Fuc-TVII and vice versa. Nevertheless, the percentages of Fuc-TVII-expressing CD4^+ or CD8^+ cells were close but not identical to those of CLA-expressing cells. According to the expression pattern of CLA and Fuc-TVII, the CD4^+ and CD8^+T cells consisted of three populations : Fuc-TVII^+CLA^+, Fuc-TVII^-CLA^+, and Fuc-TVII^+CLA^-. Our kinetics studies after in vitro stimulation with anti-CD3 mAb showed that the Fuc-TVII^-CLA^+ subset represents early activated cells that eventually differentiate into the Fuc-TVII^+CLA^+ subset and subsequently the Fuc-TVII^-CLA^+ subset. The capability of CD4^+ T cells to bind E-selectin was detected in the Fuc-TVII^+CLA^- and Fuc-TVII^+CLA^+ subset, but not the Fuc-TVII^- CLA^+. The Fuc-TVII^- CLA^+ subset was observed in increased number in PBMC coincident with resolution of the immune activation in various inflammatory settings.

  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Shiohara,T. et al.: "Gamma-delta T cells with lmphasis on their functional role in the epidermis"Chem Immunol. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mijukawa,Y. et al.: "Immunohistochemical detection of skin-homing T cells expressing fucosyltransferase VII(Fue-TVII)in vitro and in situ"Lab Invest. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mijukawa,Y. et al.: "Virus-induced immune dysregulation as a friggening factor for the development of drug rashes and autoimmure diseases"J Dermatol Sci. 22. 169-180 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Teraki,Y. et al.: "Increased circulating skin-homing lymphocyte-associated antigen(CLA)^+type 2 cytokine-producing cells, and decreased CLA^+type 1 cylokine-prodecing cells"Br J Dermatol. 143. 373-378 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shiohara,T.: "Viral infections, allergy and antoimmunity : a complex but fascinating link."J Dermatol Sci. 22. 149-151 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakayama,F. et al: "Expression of cutaneous lymphocyte-associated antigen regulated by a set of glycosyl transferases in human T cells."J Invest Dermatol. 115. 299-306 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shiohara T, Mizukawa Y, Teraki Y, Fukuda T: "Gamma-delta T cells with emphasis on their functional role in the epidermis."Chem Immunol. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mizukawa Y, Yamazeki Y, Narimatsu H, Shiohara T: "Immunohistochemical detection of skin-homing T cells expressing fucosyltransferase VII (Fuc-TVII) in vitro and in situ."Lab Invest. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mizukawa Y, Shiohara T: "Virus-induced immune dysregulation as a triggering factor for the development of drug rashes and autoimmune diseases : with emphasis on EB virus, human herpesvirus 6 and hepatitis C virus."J Dermatol Sci. 22. 169-180 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Teraki Y, Hotta T, Shiohara T: "Increased circulating skin-homing lymphocyte-associated antigen (CLA)+type 2 cytokine-producing cells, and decreased CLA+type 1 cytokine-producing cells in atopic dermatitis."Br J Dermatol. 143. 373-378 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kano Y, Shiohara T: "Current understanding of cytomegalovirus infection in immunocompetent individuals."J Dermatol Sci. 22. 196-204 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shiohara T: "Viral infections, allergy and autoimmunity : a complex, but fascinating link."J Dermatol Sci. 22. 149-151 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakayama F, Teraki Y, Mizukawa Y, Shiohara T Narimatsu H: "Expression of cutaneous lymphocyte-associated antigen regulated by a set of glycosyltranferases in human T cells : involvement of α1,3-fucosyltransferase VII and β1,4-galactosyltransferase I."J Invest Dermatol. 115. 299-306 (2000)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2002-03-26  

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