• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2001 Fiscal Year Final Research Report Summary

Development of knockout mouse of desmoyokin(AHNAK)and analysis of its functio

Research Project

Project/Area Number 11470186
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Dermatology
Research InstitutionKurume University School of Medicine(Department of Dermatology)

Principal Investigator

MORI Osamu  Kurume University School of Medicine, Dermatology, Professor, 医学部, 教授 (20129597)

Co-Investigator(Kenkyū-buntansha) NAKAMA Takekuni  Kurume University School of Medicine, Dermatology, Associated Professor, 医学部, 講師 (50221453)
KUSUHARA Masahiro  Kurume University School of Medicine, Dermatology, Associated Professor, 医学部, 講師 (40195441)
MORI Osamu  Kurume University School of Medicine, Dermatology, Associated Professor, 医学部, 助教授 (10175630)
ITO Kyogo  Kurume University School of Medicine, Immunology, Professor, 医学部, 教授 (50125499)
Project Period (FY) 1999 – 2001
KeywordsDesmosome / Knockoit mouse / Desincykin / AHNAK / Keratinocyte / BS cell / Cell adhesion / molecular biclogy
Research Abstract

In the present study, in order to clarify the role of the molecule (desmoyokin/AHNAK protein), we successfully established gene targetting mice (knockout mice) of desmoyokin/AHNAK protein. We screened mouse genomic DNA from mouse genomic DNA library, and prepared a targetting vector for desmoyokin/AHNAK protein. We inserted linealized targetting vector into enbryonic stem cells, and screened homologous recombinated cells. Using the homologous recombinated enbryonic stem cells, we prepare heterozygous mice and finally obtained homozygous knockout mice of desmoyokin/AHNAK protein. We have confirmed by both DNA and protein levels that these targetted mice are really lacking desmoyokin/AHNAK protein. At the same time, we also suceeded in preparation of desmoyokin/AHNAK protein knockout enbryonic stem cells, which should also be useful to examine the role of desmoyokin/AHNAK protein in the cell levels. We will examine the role of desmoyokin/AHNAK protein using these desmoyokin/AHNAK protein knockout mice and knockout enbryonic stem cells. These study should provide us with numerous important insights into the misterious giant protein, desmoyokin/AHNAK protein.

  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Nousari HC, Deterding R, Wojtczack H, Aho S, Uitto J, Hashimoto T, Anhalt GJ: "The mechanism of respiratory failure in paraneoplastic pemphigus"N Eng J Med. 340. 1406-1410 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nie Z, Wang N, Amagai M, Hashimoto T: "C-terminus of Desmoyokin/AHNAK protein is mainly located in the nucleus in low calcium condition, but low to normal calcium shift translocates a part of it to cytoplasm"J Invest Dermatol. 114. 1044-1049 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] O'Toole EA, Mak LL, Guitart J, Woodley DT, Hashimoto T, Amagai M, Chan LS: "Induction of keratinocyte interleukin-8 expression and secretion by lgG autoantibodies as a novel mechanism of epidermal neutrophil recruitment in a pemphigus variant"Clin Exp Immunol. 119. 217-224 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nagata Y, Karashima T, Watt FM, Salmhofer W, Kanzaki T, Hashimoto T: "Paraneoplastic pemphigus sera react strongly with multiple epitopes on the various regions of envoplakin and periplakin, except for C-terminal homologous domain of periplakin"J Invest Dermatol. 116. 556-563 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hashimoto T, Komai A, Futei Y, Nishikawa T, Amagai M: "Detection of IgA auoantibodies to desmogleins by an enzyme-lined immunosorbent assay -The presence of new minor subtypes of IgA pemphigus"Arch Dermatol. 137. 735-738 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hamada T, Nagata Y, Tomita M Salmhofer W, Hashimoto T: "Bullous pemphigoid sera react specifically with various domains of BP230, most frequently with C-terminal domain, by immunoblot analyses using bacterial recombinant proteins covering entire molecule"Exp Dermatol. 10. 256-263 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hashimoto T: "Clin Dermatol 19"Immunopathology of paraneoplastic pemhigus. 675-682 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nousari HC, Deterding R, Wojtczapk H, Aho S, Uitto J, Hashimoto T, Anhalt GJ: "The mechanism of respiratory failure in paraneoplastic pemphigus."N Eng J Med. 340. 1406-1410 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nie Z, Wang N, Amagai M, Hashimoto T: "C-terminus of Desmoyokin/AHNAK protein is mainly located in the nucleus in low calcium condition, but low to normal calcium shift translocates a part of it to cytoplasm."J Invest Dermatol. 114. 1044-1049 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] OToole EA, Mak LL, Guitart J, Woodley DT, Hashimoto T, Amagai M, Chan LS: "lnduction of keratinocyte interleukin-8 expression and secretion by IgG autoantibodies as a novel mechanism of epidermal neutrophil recruitment in a pemphigus variant."Clin Exp Immunol. 119. 217-224 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nagata Y, Karashima T, Watt FM, Salmhofer W, Kanzaki T, Hashimoto T: "Paraneoplastic pemphigus sera react strongly with multiple epitopes on the various regions of envoplakin and periplakin, except for C-termina! homologous domain of periplakin."J Invest Dermatol. 116. 556-563 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hashimoto T, Komai A, Futei Y, Nishikawa T, Amagai M: "Detection of IgA auoantibodies to desmogleins by an enzyme-lined immunosorbent assay -The presence of new minor subtypes of IgA pemphigus."Arch Dermatol. 137. 735-738 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hamada T, Nagaja Y, Tomita M, Salmhofer W, Hashimoto T: "Bullous pemphigoid sera react specifically with various domains of BP230, most frequently with C-terminal domain, by immunoblot analyses using bacterial recombinant proteins covering the entire molecule."Exp Dermatol. 10. 256-263 (2001)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2003-09-17  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi