2000 Fiscal Year Final Research Report Summary
SIGNIFICANCE OF APOPTOSIS IN TUMOR REOXYGENATION
| Project/Area Number |
11470197
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | NATIONAL INSTITUTE OF RADIOLOGICAL SCIENCES |
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Principal Investigator |
ANDO Koichi National Institute of Radiological Science, Researcher, 国際宇宙放射線医学研究センター, 研究員 (00159526)
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| Co-Investigator(Kenkyū-buntansha) |
MAJIMA Hideyuki National Institute of Radiological science, Researcher, 国際宇宙放射線医学研究センター, 研究員 (60165701)
AKASHI Makoto National Institute of Radiological Sciences, Researcher, 放射線障害医療研究部, 研究員 (10670979)
TANISHITA Kazuo Keio University, Department of System Design Engineering, Professor, 理工学部, 教授 (10101776)
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| Project Period (FY) |
1999 – 2000
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| Keywords | carbon ions / oxygen consumption / OER / PR-350 / LET / MnSOD / transription factor / H2O2 |
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| Research Abstract |
The murine NFSa fibrosarcomas under condition of clamped hypoxia developed less apoptosis at 2 days after irradiation than those under air-breathing condition. The clamped hypoxia did not, however, affect apoptosis developing 3 days or later, suggesting that several mechanisms are involved in tumor reoxygenation. The oxygen consumption of tumor cells was more effectively reduced by high let carbon ions than X rays, suggesting a possibility that the accelerated reoxygenation by carbon irradiation could be due to the reduced oxygen consumption. Apoptosis of cultured V79 Chinese hamster cells after irradiation showed that the oxygen enhancement ration(OER) for carbon ions and X rays was the same, i.e., 2.5. A hypoxic radiosensitizer PR-350 enhanced apoptosis of hypoxic V79 cells after X-ray irradiation, but not after carbon-ion irradiation. When colony formation was studied, OER of V79 cells was 3.0 and 2.0 after X- and carbon-irradiation, respectively. The sensitizing activity of PR-350 was 1.5 and 1.3 for X- and carbon-irradiation, respectively. Apoptosis seems to be less influenced than reproductive death by oxygen condition and LET. Human monocytic leukemia, cells THP-1 responded to X-ray Irradiation such that activation of a transcription factor NFkB was associated with reduction of IkB. As the activation of NFkB is reported to inhibit TNF-mediated apoptosis, X-ray irradiation could activate both apoptosis and anti-apoptosis signaling pathway. Human ovarian cancer cells, which was transfected and over-expressing manganese superoxide dismutase (MnSOD) genes, showed retarded growth and increased radiosensitivity, comparing with vector-transfected control cells. The increased radiosensitivity was due to apoptosis which was associated with the overproduction of H2O2.
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Research Products
(48 results)
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[Journal Article] Relative Biological Effectiveness of the 235 MeV Proton Beams at the National Cancer Center Hospital East.2001
Author(s)
K.Ando, Y.Furusawa, M.Suzuki, K.Nojima, H.Majima, S.Koike, M.Aoki, WShimizu, Y.Futami, T.Ogino, S.Murayama, H.Ikeda
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Journal Title
J.Radiat.Res. 42(1)
Pages: 79-89
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] TNF-a induced p21WAF1 but not BAX in colon cancer cells WiDr with mutated p53 : important role off protein stabilization.2000
Author(s)
Kobayashi, N., Takada, Y., Hachiya, M., Ando.K., Nakajima, N., Akashi, M.
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Journal Title
Cytokine 12(12)
Pages: 1745-1754
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] IL-1 induces expression of p21(WAF1) independently of p53 in high-passage human embryonic fibroblasts WI38.2000
Author(s)
Osawa, Y., Hachiya, M., Araki, S., Kusama, T., Matsushima, K., Aoki, Y., Akashi, M.
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Journal Title
J Biochem. 127
Pages: 883-893
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Biophysical charactersitics of HIMAC clinical irradiation system for heavy-ion radiation therapy.1999
Author(s)
Tatsuaki Kanai, Masahiro Endo, Shinichi Minohara, Nobuyuki Miyahara, Hiroko Koyama-Ito, Hiromi Tomura, Naruhiro Matsufuji, Yasuyuki Futami, Akifumi Fukumura, Takeshi Hiraoka, Yoshiya Furusawa, Koichi Ando, Masao Suzuki, Fuminori Soga, Kiyomitsu Kawachi
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Journal Title
Int.J.Radiation Oncology.Biol.Phys. 44(1)
Pages: 201-210
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Theoretical strategy : How to make treatment planning for heavy ion-beam therapy ?1999
Author(s)
Hideyuki J.Majima, Masao Suzuki, Koichi Ando, Koh-ichi Sakata, Norio Suzuki, Shigefumi Okada, Shozo Suzuki, Tadaaki Miyamoto, Kazunobu Fujitaka, Hiroshi Fuji, Shigeru Yamada, Hirohiko Tsujii, Muneyasu Urano
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Journal Title
Jpn.J.Med.Phys. 19
Pages: 37-47
Description
「研究成果報告書概要(欧文)」より
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