1999 Fiscal Year Annual Research Report

異種免疫反応の抑制に関する研究

Research Project

Project/Area Number	11470256
Research Category	Grant-in-Aid for Scientific Research (B)
Research Institution	The University of Tokyo
Principal Investigator	針原康東京大学, 医学部・付属病院, 講師 (10189714)
Co-Investigator(Kenkyū-buntansha)	菅原寧彦東京大学, 医学部・附属病院, 助手 (90313155) 今村宏東京大学, 医学部・附属病院, 助手 (00283268)
Keywords	異種移植 / 心移植 / 肝移植 / concordant / discordant
Research Abstract	平成11年度は各実験モデルの確立を目指した.HamsterおよびGuinea-pigからRatへの組み合わせを小動物異種移植モデルとして,これらの組み合わせにい心移植および肝移植を施行した.心移植はOno&Lindsay法に準じて,腹腔内に異所性に移植した.また肝移植はKamada法に準じて,カフ法を門脈と肝下部下大静脈の吻合に使用して,局所性に移植した.HamsterからRatへの組み合わせ(concordant)の心移植では無治療ではグラフト生着期間は2-3日であり,80%以上の収率が得られ,実験モデルとして十分と考えられた.またHamsterからRatへの組み合わせ(concordant)の肝移植では無治療ではグラフト生着期間は4-7日であり,65%程度の収率であった.実験モデルとして使用可能と考えられた.Guinea-pigからRatへの組み合わせ(discordant)の心移植では無治療では14分以内に移植心は拍動を停止した.この組み合わせでは無治療では生着期間は短いが実験モデルとして使用可能と考えられた.Guinea-pigからRatへの組み合わせ(discordant)の肝移植については現時点では収率が不十分であり,手技の確立を目指して検討中である. ラット補体抑制因子512遺伝子をHVJliposome法を用いてHamsterおよびGuinea-pig内皮細胞への導入する準備を進めている.まずドナー臓器摘出前の投与によるこれら遺伝子の内皮細胞導入(心および肝)について効率の最も良い条件を確定できる予定である.

Research Products
(11 results)

All Other

All Publications (11 results)

[Publications] Sakamoto Y,Harihara Y,Nakatsuka T,Kawarasaki H,Takayama T,Kubota K,Kimura W,Kita Y,Hashizume K,Makuuchi M: "Rescue of liver grafts from hepatic artery occlusion in living-related liver transplantation"British Journal of Surgery. 86. 886-889 (1999)
[Publications] Harihara Y,Makuuchi M,Kawarasaki H,Takayama T,Kubota K,Hirata M,Kita Y,Kusaka K,Sano K,Hashizume K: "Portal venoplasty for recipients in living-related liver transplantation"Transplantation. 68(8). 1199-1200 (1999)
[Publications] Nakatsuka T,Takushima A,Harihara Y,Makuuchi M,Kawarasaki H,Hashizume K: "Versatility of the inferior epigastric artery as an interpositional vascular graft in living-related liver transplantation"Transplantation. 67(11). 1490-1492 (1999)
[Publications] Matsukura A,Kita Y,Harihara Y,Tanaka H,Ito M,Kawarasaki H,Kubota K,Takayama T,Hashizume K,Makuuchi M: "Does peripheral blood eosinophilia predict allograft rejection in living-related liver transplantation?"Transplantation Proceedings. 31(7). 2729-2730 (1999)
[Publications] Harihara Y,Makuuchi M,Kawarasaki H,Takayama T,Kubota K,Ito M,Tanaka H,Yoshino H,Hirata M,Kita Y,Kusaka K,Sano K,Saiura A,Ijichi M,Matsukura A,Watanabe K,Hashizume K,Nakatsuka T: "Effect of fluconazole on blood level of Tacrolimus"Transplantation Proceedings. 31(7). 2767-2767 (1999)
[Publications] Harihara Y,Makuuchi M,Kawarasaki H,Takayama T,Kubota K,Ito M,Yoshino H,Tanaka M,Hirata H,Kita Y,Kusaka K,Sano K,Ijichi M,Watanabe M,Hashizume A,Nakatsuka T: "Initial experience with 40 cases of living-related liver transplantation at the University of Tokyo"Transplantation Proceedings. 31(7). 2893-2894 (1999)
[Publications] Matsukura A,Kita Y,Harihara Y,Kubota K,Takayama T,Kawarasaki H,Hashizume K,Makuuchi M: "Is splenic artery ligation effective for thrombocytopenia early after liver transplantation?"Transplantation Proceedings. 31(7). 2906-2907 (1999)
[Publications] Hirata M,Harihara Y,Sano K,Kusaka K,Kita Y,Nakao A,Taniguchi S,Seki G,Yoshino H,Ito M,Kubota K,Takayama T,Kawarasaki H,Hashizume K,Makuuchi M: "Recovery of renal function after living-related liver transplantation in a case of HBV liver cirrhosis with renal failure"Transplantation Proceedings. 31(7). 2904-2905 (1999)
[Publications] Kita Y,Sakakura H,Hirata M,Harihara Y,Tanaka H,Ito M,Yoshino H,Takayama T,Kubota K,Hashizume K,Makuuchi M: "Ursodeoxycholic acid in serum and liver tissue in patient with end-stage cholestatic cirrhosis"Transplantation Proceedings. 31(7). 2897-2898 (1999)
[Publications] Tanaka H,Harihara Y,Kita Y,Kawarasaki H,Hashizume K,Takayama T,Makuuchi M: "Changes in graft volume after living-related liver transplantation"Transplantation Proceedings. 31(7). 2895-2896 (1999)
[Publications] Hirata M,Saito S,Nishimura M,Sano K,Kusaka K,Kita Y,Harihara Y,Yoshino H,Ito M,Kawarasaki H,Hashizume K,Makuuchi M: "Changes in natural killer cell activity before and after living-related donor liver transplantation"Transplantation Proceedings. 31(7). 2701-2703 (1999)

1999 Fiscal Year Annual Research Report

異種免疫反応の抑制に関する研究

Principal Investigator

針原 康 東京大学, 医学部・付属病院, 講師 (10189714)

Research Products

[Publications] Sakamoto Y,Harihara Y,Nakatsuka T,Kawarasaki H,Takayama T,Kubota K,Kimura W,Kita Y,Hashizume K,Makuuchi M: "Rescue of liver grafts from hepatic artery occlusion in living-related liver transplantation"British Journal of Surgery. 86. 886-889 (1999)

[Publications] Harihara Y,Makuuchi M,Kawarasaki H,Takayama T,Kubota K,Hirata M,Kita Y,Kusaka K,Sano K,Hashizume K: "Portal venoplasty for recipients in living-related liver transplantation"Transplantation. 68(8). 1199-1200 (1999)

[Publications] Nakatsuka T,Takushima A,Harihara Y,Makuuchi M,Kawarasaki H,Hashizume K: "Versatility of the inferior epigastric artery as an interpositional vascular graft in living-related liver transplantation"Transplantation. 67(11). 1490-1492 (1999)

[Publications] Matsukura A,Kita Y,Harihara Y,Tanaka H,Ito M,Kawarasaki H,Kubota K,Takayama T,Hashizume K,Makuuchi M: "Does peripheral blood eosinophilia predict allograft rejection in living-related liver transplantation?"Transplantation Proceedings. 31(7). 2729-2730 (1999)

[Publications] Harihara Y,Makuuchi M,Kawarasaki H,Takayama T,Kubota K,Ito M,Tanaka H,Yoshino H,Hirata M,Kita Y,Kusaka K,Sano K,Saiura A,Ijichi M,Matsukura A,Watanabe K,Hashizume K,Nakatsuka T: "Effect of fluconazole on blood level of Tacrolimus"Transplantation Proceedings. 31(7). 2767-2767 (1999)

[Publications] Matsukura A,Kita Y,Harihara Y,Kubota K,Takayama T,Kawarasaki H,Hashizume K,Makuuchi M: "Is splenic artery ligation effective for thrombocytopenia early after liver transplantation?"Transplantation Proceedings. 31(7). 2906-2907 (1999)

[Publications] Kita Y,Sakakura H,Hirata M,Harihara Y,Tanaka H,Ito M,Yoshino H,Takayama T,Kubota K,Hashizume K,Makuuchi M: "Ursodeoxycholic acid in serum and liver tissue in patient with end-stage cholestatic cirrhosis"Transplantation Proceedings. 31(7). 2897-2898 (1999)

[Publications] Tanaka H,Harihara Y,Kita Y,Kawarasaki H,Hashizume K,Takayama T,Makuuchi M: "Changes in graft volume after living-related liver transplantation"Transplantation Proceedings. 31(7). 2895-2896 (1999)

[Publications] Hirata M,Saito S,Nishimura M,Sano K,Kusaka K,Kita Y,Harihara Y,Yoshino H,Ito M,Kawarasaki H,Hashizume K,Makuuchi M: "Changes in natural killer cell activity before and after living-related donor liver transplantation"Transplantation Proceedings. 31(7). 2701-2703 (1999)

針原康東京大学, 医学部・付属病院, 講師 (10189714)