2000 Fiscal Year Final Research Report Summary
Human Liver Cell and Pancreatic Cell Transplantation-Study Using Resected Liver and Pancreas Segment-
| Project/Area Number |
11470262
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Showa University |
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Principal Investigator |
KUSANO Mitsuo Showa University, Dept.of Surgery, School of Medicine Showa University, 教授 (70091569)
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| Co-Investigator(Kenkyū-buntansha) |
ISHII Hiroshi Showa University, Dept.of Surgery, School of Medicine, 講師 (50255725)
MURAKAMI Masahiko Showa University, Dept.of Surgery, School of Medicine, 講師 (70255727)
ITO Yoji Showa University, Dept.of Surgery, School of Medicine, 講師 (10151549)
AOKI Takeshi Showa University, Dept.of Surgery, School of Medicine, 助手 (30317515)
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| Project Period (FY) |
1999 – 2000
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| Keywords | hepatocye transplantation / islet cell transplantation / liver regeneration / regenerative medicine / liver support system / pancreatic function support |
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| Research Abstract |
Hepatocyte or pancreatic islets cell transplantations have investigating as an alternative to liver or pancreas transplantation in patients with liver-based metabolic disorders or with severe pancreatitis and diabetes mellitus. Hepatocyte transplantation is making its transition from bench to bedside for acute and chronic liver failure. More than 80 patients have now been transplanted world wide.
A major limiting factor is the availability of good quality cells as human hepatocytes are derived from resected grafts. There is also a need to evaluate the mechanism of liver regeneration that will improve the survival and function of transplanted human hepatocytes. Such approach may allow hepatocyte transplantation to become an accepted and practical alternative to liver transplantation in the near future. To realize this procedure, a hepatocyte bank system capable of supplying large numbers of hepatocytes must be established. We previously reported an easy method for cryopreserving hepatocy
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tes using a microencapsulation technique. Here, we investigated how cryoinjury to microencapsulated hepatocytes could be avoided during cryopreservation. Cryomicroscopy showed that the alginate microencapsulation technique protected the hepatocytes from physical damage caused by the growth of extracellular ice crystals. Ultrastructural examination revealed that the intracellular environment of the microencapsulated hepatocytes was maintained. Our microencapsulation technique protects hepatocytes from cryoinjury. This novel technique could be utilized by hepatocyte banks.
The regulation of liver regeneration after partial hepatectomy (PHx) is complex and involves many different cytokines. We investigated the role of one of these, transforming growth factor-beta1 (TGF-beta1), an inhibitor of liver regeneration. Livers from treated animals contained an increased number of cells in S phase. Antibody administration 24 h after PHx resulted in the highest peak of proliferation ; moreover, peak MIB-5 labeling was also observed at that time. However, neither residual liver-weight-to-body-weight ratios nor regeneration rates differed significantly between any of the animals. These results indicate that TGF-beta1 may inhibit the G1 checkpoint, and serum TGF-beta1 concentration may influence HGF to regulate liver regeneration and to maintain homeostasis of proliferation after PHx. These investigations willl promote hepatocyte or pancreatic cell transplantaion in near future. Less
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Research Products
(19 results)
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[Journal Article] Cold preservation of the liver with oxygenation by a two-layer method2009
Author(s)
Odaira M, Aoki T, Miyamoto Y, Yasuhara R, Jin Z, Yu J, Nishino N, Yamada K, Kusano T, Hayashi K, Yasuda D, Koizumi T, Mitamura K, Enami Y, Niiya T, Murai N, Kato H, Shimizu Y, Kamijyo R, Kusano M.
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Journal Title
J Surg Res. 152(2)
Pages: 209-17
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Microencapsule technique protects hepatocytes from cryoinjury2008
Author(s)
Kusano T, Aoki T, Yasuda D, Matsumoto S, Jin Z, Nishino N, Hayashi K, Odaira M, Yamada K, Koizumi T, Izumida Y, Mitamura K, Enami Y, Niiya T, Murai N, Kato H, Shimizu Y, Kou K, Furukawa Y, Matsusita M, Todo S, Shioda S, Kusano M.
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Journal Title
Hepatol Res. 38(6)
Pages: 593-600
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Hepatocyte transplantation from steatotic liver in a rat model2007
Author(s)
Hayashi K, Aoki T, Jin Z, Wang H, Nishino N, Kusano T, Yasuda D, Koizumi T, Enami Y, Odaira M, Yamada K, Mitamura K, Niiya T, Murai N, Kato H, Shimizu Y, Kusano M.
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Journal Title
J Surg Res. 142(1)
Pages: 104-12
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Long-term maintenance of the drug transport activity in cryopreservation of microencapsulated rat hepatocytes2007
Author(s)
Koizumi T, Aoki T, Kobayashi Y, Yasuda D, Izumida Y, Jin Z, Nishino N, Shimizu Y, Kato H, Murai N, Niiya T, Enami Y, Mitamura K, Yamamoto T, Kusano M.
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Journal Title
Cell Transplant 16(1)
Pages: 67-73
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] A novel method of cryopreservation of rat and human hepatocytes by using encapsulation technique and possible use for cell transplantation2005
Author(s)
Aoki T, Koizumi T, Kobayashi Y, Yasuda D, Izumida Y, Jin Z, Nishino N, Shimizu Y, Kato H, Murai N, Niiya T, Enami Y, Mitamura K, Yamamoto T, Kusano M.
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Journal Title
Cell Transplant 14(9)
Pages: 609-20
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Intrasplenic transplantation of encapsulated hepatocytes decreases mortality and improves liver functions in fulminant hepatic failure from 90% partial hepatectomy in rats2005
Author(s)
Aoki T, Jin Z, Nishino N, Kato H, Shimizu Y, Niiya T, Murai N, Enami Y, Mitamura K, Koizumi T, Yasuda D, Izumida Y, Avital I, Umehara Y, Demetriou AA, Rozga J, Kusano M.
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Journal Title
Transplantation 79(7)
Pages: 783-90
Description
「研究成果報告書概要(欧文)」より
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