2001 Fiscal Year Annual Research Report

歯の発生におけるBMPファミリーおよびそのレセプターの遺伝子発現と機能解析

Research Project

Project/Area Number	11470377
Research Institution	OKAYAMA UNIVERSITY
Principal Investigator	長塚仁岡山大学, 大学院・医歯学総合研究科, 助教授 (70237535)
Co-Investigator(Kenkyū-buntansha)	GUNDUZ Mehmet 岡山大学, 大学院・医歯学総合研究科, 助手 (70333507) 辻極秀次岡山大学, 大学院・医歯学総合研究科, 助手 (70335628) 永井教之岡山大学, 大学院・医歯学総合研究科, 教授 (90085770) 長岡紀幸岡山大学, 歯学部, 教務員 (70304326)
Keywords	歯胚 / BMP / mRNA / In situ hybridization / 成長因子
Research Abstract	器官形成の上皮間葉相互作用に関わる因子として注目されているBMPファミリーが,歯胚分化過程のいかなる時期で発現しているかをin situ hybridization法を用いて検索した。 BMP-2,3,4,5,6,7のそれぞれに特異的なディゴキシゲニン標識cRNAプローブを作製し,胎生11日から生後1日までのマウス歯胚に対して非放射性in situ hybridization法を行った。胎生11日にすでに発現が見られたのはBMP-4および7であり,胎生12日になるとBMP-2の発現も見られた。これらの蛋白を免疫組織化学的に検索した結果ほぼ遺伝子発現領域に一致した分布を示した。BMP-2,4,7は歯牙発生の初期から上皮間葉相互作用による形態形成および細胞分化に関与すると考えられた。BMP-5は胎生17日の帽状期後期の内エナメル上皮でその発現が観察され,エナメル質あるいは象牙質形成に重要な役割を果たしている可能性が考えられた。一方,歯胚基底膜の主要構成成分であるIV型コラーゲンのα1からα6までの6種類のα鎖の歯牙発生過程での局在と消長を免疫組織学的に観察した結果、歯胚上皮基底膜にα鎖の部位および時期特異的な局在と組み合わせが存在することが明らかになった。これらの結果は基底膜と成長因子の共同作用が初期歯胚形態形成、細胞分化に関与している可能性を示唆している。歯胚発育過程において,BMPと密接な関連を有するcbfalの関与について検索を行った。その結果,Cbfalノックアウトマウスにおける臼歯の歯胚発育は、組織学的には帽状期で停止していたが,切歯では基質蛋白の遺伝子発現の面からは象牙芽細胞およびエナメル芽細胞にまで分化しており,臼歯とは異なるシグナル伝達系が存在する可能性が考えられた。

Research Products

(15 results)

All Other

All Publications (15 results)

[Publications] Noriyuki NAGAI: "Localization of type IV collagen α-1 to α-6 chains in basement membrane during mouse molar germ development"The International Journal of Developmental Biology. 45・7. 827-831 (2001)
[Publications] Hitoshi NAGATSUKA: "Quantitation of epidermal growth factor gene amplification by competitive polymerase reaction in premalignant and malignant oral epithelial lesions"Oral Oncology. 30・6. 599-604 (2001)
[Publications] Eiki YAMACHIKA: "Identification of a stop codon mutation in the CBFA1 runt domain from a patient with cleidoranial dysplasia and cleft lip"Journal of Oral Pathology and Medicine. 30・6. 381-383 (2001)
[Publications] Hitoshi NAGATSUKA: "Site dependence of Bone Formation Induced by rhBMP-2 : Comparing Rat Parietal Bone and Heterotopic Back Region"Engineering Materials. 192-195. 511-514 (2001)
[Publications] Akira YAMASAKI: "Expression of Type I, Type II, and-Type X Collagen Genes during Altered Endochondral Ossification in the Femoral Epiphysis of Osteosclerotic (oc/oc) Mice"Calcified Tissue International. 68. 53-60 (2001)
[Publications] Yoshiya UEYAMA: "Initial tissue response to anti-washout apatite cement in the rat palatal region : comparison with conventional apatite"Journal of Biomedical Materials Research. 55・4. 652-660 (2001)
[Publications] N.MIZUKAWA: "Human alpha-and beta-defensin immunoreactivity in oral mucoepidermoid carcinomas"Anticancer Research. 21. 2171-2174 (2001)
[Publications] 辻極秀次: "rhBMP-2/コラーゲン固定化によるST2細胞への影響とシグナル伝達系遺伝子の発現"岡山歯学学会雑誌. 20・1. 42-55 (2001)
[Publications] K.NISHIDA: "Involvement of nitric oxide in chondrocyte cell death in chondro-osteophyte formation"Osteoarthritis and Cartilage. 9・3. 232-237 (2001)
[Publications] O.MITSUKUNI: "Pronostic Significance of p27 and Ki-67 Expression in Mucoepidermoid Carcinima of the Intraoral Minor Salivary Gland"Modem Pathology. 14・10. 1008-1014 (2001)
[Publications] Masaru MURATA: "Combination of Recombinant Human BMP-2 and Atelocollagen Implant with Cortical perforations Bone Augmentation"Journal of Hard Tissue Biology. 10・1. 1-6 (2001)
[Publications] Y.J.LEE: "Phenomelanin and Eumelanin of Malignant Melanomas and Melanosis in Oral Mucosa"Journal of Hard Tissue Biology. 10・2. 89-95 (2001)
[Publications] K.SUGATA: "Genetic Alteration of Penicillin Non-Susceptible Streptococcus pneumoniae Observed throughout Recurrence of Acute Otitis Media Detected by Amplified Fragment Length Polymorphism Analysis"Acta Medica Okayama. 55・3. 167-174 (2001)
[Publications] Y.YASUOKA: "Combination of tumor necrosis factor-alpha and interferon-alpha induces apoptotic cell death through a c-myc dependent pathway in p53 mutant H226br non-small cell lung cancer cell line"Experimental Cell Research. 271. 214-222 (2001)
[Publications] Tsohiyuki KAWAKAMI: "Characteristics of Bone Morphogenetic Protein-induced Chondroid Bone : Histochemical, Immunohistochemical and in situ Hybridization Examinations"The Journal of International Medical Research. 29・6. 480-487 (2001)

2001 Fiscal Year Annual Research Report

歯の発生におけるBMPファミリーおよびそのレセプターの遺伝子発現と機能解析

Principal Investigator

長塚 仁 岡山大学, 大学院・医歯学総合研究科, 助教授 (70237535)

Research Products

[Publications] Noriyuki NAGAI: "Localization of type IV collagen α-1 to α-6 chains in basement membrane during mouse molar germ development"The International Journal of Developmental Biology. 45・7. 827-831 (2001)

[Publications] Hitoshi NAGATSUKA: "Quantitation of epidermal growth factor gene amplification by competitive polymerase reaction in premalignant and malignant oral epithelial lesions"Oral Oncology. 30・6. 599-604 (2001)

[Publications] Eiki YAMACHIKA: "Identification of a stop codon mutation in the CBFA1 runt domain from a patient with cleidoranial dysplasia and cleft lip"Journal of Oral Pathology and Medicine. 30・6. 381-383 (2001)

[Publications] Hitoshi NAGATSUKA: "Site dependence of Bone Formation Induced by rhBMP-2 : Comparing Rat Parietal Bone and Heterotopic Back Region"Engineering Materials. 192-195. 511-514 (2001)

[Publications] Akira YAMASAKI: "Expression of Type I, Type II, and-Type X Collagen Genes during Altered Endochondral Ossification in the Femoral Epiphysis of Osteosclerotic (oc/oc) Mice"Calcified Tissue International. 68. 53-60 (2001)

[Publications] Yoshiya UEYAMA: "Initial tissue response to anti-washout apatite cement in the rat palatal region : comparison with conventional apatite"Journal of Biomedical Materials Research. 55・4. 652-660 (2001)

[Publications] N.MIZUKAWA: "Human alpha-and beta-defensin immunoreactivity in oral mucoepidermoid carcinomas"Anticancer Research. 21. 2171-2174 (2001)

[Publications] 辻極秀次: "rhBMP-2/コラーゲン固定化によるST2細胞への影響とシグナル伝達系遺伝子の発現"岡山歯学学会雑誌. 20・1. 42-55 (2001)

[Publications] K.NISHIDA: "Involvement of nitric oxide in chondrocyte cell death in chondro-osteophyte formation"Osteoarthritis and Cartilage. 9・3. 232-237 (2001)

[Publications] O.MITSUKUNI: "Pronostic Significance of p27 and Ki-67 Expression in Mucoepidermoid Carcinima of the Intraoral Minor Salivary Gland"Modem Pathology. 14・10. 1008-1014 (2001)

[Publications] Masaru MURATA: "Combination of Recombinant Human BMP-2 and Atelocollagen Implant with Cortical perforations Bone Augmentation"Journal of Hard Tissue Biology. 10・1. 1-6 (2001)

[Publications] Y.J.LEE: "Phenomelanin and Eumelanin of Malignant Melanomas and Melanosis in Oral Mucosa"Journal of Hard Tissue Biology. 10・2. 89-95 (2001)

[Publications] K.SUGATA: "Genetic Alteration of Penicillin Non-Susceptible Streptococcus pneumoniae Observed throughout Recurrence of Acute Otitis Media Detected by Amplified Fragment Length Polymorphism Analysis"Acta Medica Okayama. 55・3. 167-174 (2001)

[Publications] Y.YASUOKA: "Combination of tumor necrosis factor-alpha and interferon-alpha induces apoptotic cell death through a c-myc dependent pathway in p53 mutant H226br non-small cell lung cancer cell line"Experimental Cell Research. 271. 214-222 (2001)

[Publications] Tsohiyuki KAWAKAMI: "Characteristics of Bone Morphogenetic Protein-induced Chondroid Bone : Histochemical, Immunohistochemical and in situ Hybridization Examinations"The Journal of International Medical Research. 29・6. 480-487 (2001)

長塚仁岡山大学, 大学院・医歯学総合研究科, 助教授 (70237535)