Isolation of a novel tumor suppressor gene on the Chromosome 11 associated with oral squamous cell carcinoma.

2001 Fiscal Year Final Research Report Summary

• Project/Area Number: 11470430
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Chiba University
• Principal Investigator: YOKOE Hidetaka Chiba University, University Hospital, Lecturer, 医学部・附属病院, 講師 (70261930)
• Co-Investigator(Kenkyū-buntansha): SHIRASAWA Hiroshi Chiba University, Graduate School of Medicine, Professor, 大学院・医学研究院, 教授 (00216194) ; SUZUKI Nobuo Chiba University, Graduate School of Medicine, Professor, 大学院・医学研究院, 教授 (90111426) ; TANZAWA Hideki Chiba University, Graduate School of Medicine, Professor, 大学院・医学研究院, 教授 (50236775) ; UZAWA Katsuhiro Chiba University, Graduate School of Medicine, Associate Professor, 大学院・医学研究院, 助教授 (30302558)
• Project Period (FY): 1999 – 2001
• Keywords: Chromosome 11q25 / LOH / tumor suppressor gene / oral cancer

Research Abstract

To search for the existence of tumor-suppressor gene(s) associated with oral squamous cell carcinoma (SCC) on chromosome 11, we have performed high-resolution deletion mapping in 31 patients with oral SCC using 22 microsatellite markers for this chromosomal region. Our data show 2 distinct, commonly deleted regions. The first region, with frequent loss of heterozygosity (LOH), was restricted between markers D11S939 and D11S924 separated by 3 centimorgans (cM) on chromosome 11q23. The second region of common deletion was identified between markers D11S912 and D11S910, separated by 7 cM at 11q25. Our results suggest tnat at least 2 tumor-suppressor genes involved in the development of oral SCC are present on the long arm of chromosome 11. To identify the candidate tumor suppressor genes at chromosome 11q23 and 11q25, differential display method and representational difference analysis, using tumors with and without LOH at these loci. Four DNA fragments were isolated and one of them was confirmed the presence on chromosome 11q. The sequence of the DNA fragment was detected and it was considered as a part of a putative tumor suppressor gene involved in the oncogenesis and development of oral squamous cell carcinoma.