2000 Fiscal Year Final Research Report Summary
ESTABLISHMENT AND APPLIANCE OF NEWLY GENE THERAPY FOR DEGENERATIVE ARTHROSIS USING DECOY STRATEGY
| Project/Area Number |
11470440
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
TAKENOSHITA Yasuharu KYUSHU UNIVERSITY GRADUATE SCHOOL OF DENTAL SCIENCE, ASS PROF, 歯学研究院, 助教授 (50117157)
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| Co-Investigator(Kenkyū-buntansha) |
KUBOTA Yasutaka KYUSHU UNIVERSITY GRADUATE SCHOOL OF DENTAL SCIENCE, ASS PROF, 歯学研究院, 助手 (60205151)
NAKAGAWA Kazunori KYUSHU UNIVERSITY GRADUATE SCHOOL OF MEDICAL SCIENCE, ASS PROF, 医学研究院, 講師 (50217668)
ISHIBASHI Hiroaki KYUSHU UNIVERSITY GRADUATE SCHOOL OF DENTAL SCIENCE, ASS PROF, 歯学研究院, 助手 (90254630)
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| Project Period (FY) |
1999 – 2000
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| Keywords | TEMPOROMANDIBULAR JOINT / EXTRACELLULAR MATRIX / GENE THERAPY |
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| Research Abstract |
(l) Examination for the expression of uPA and MMPs stimulated by several cytokines using cultured cells.
The expression of uPA and MMPs was increased by the stimulation with TNFα using cultured cells derived from cartilaginous tissue of TMJ.
(2) Examination of transcriptional factor related to cytokines stimulation. Transcriptional factors, NF-kB and Sp1, were activated by TNFα stimulation at the cultured cells derived from cartilaginous tissue.
(3) Establishment of the condition for the gene transfer
We established the condition for the Decoy oligodeoxynucletides targeted to NF-kB and Sp1 using HVJ-liposome method, and decoy ODNs were routinely transferred to 1OO % of the cells.
(4) Inhibition of the expression of uPA and MMPs by Decoy ODNs transfection
The expression of the uPA and MMPs were inhibited by the transfection of Decoy ODNs to the cultured cells derived from cartilaginous tissue.
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