2001 Fiscal Year Final Research Report Summary
ANALYSIS OF ANTI-MICROBIAL FACTORS OF SALIVA IN ELDERLY
| Project/Area Number |
11470459
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
矯正・小児・社会系歯学
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| Research Institution | NATIONAL INSTITUTE OF INFECTIOUS DISEASES |
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Principal Investigator |
HANADA Nobohiro NATIONAL INSTITUTE OF INFECTIOUS DISEASES, 口腔科学部, ORALSCIENCE DIRECTOR (70180916)
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| Co-Investigator(Kenkyū-buntansha) |
ETO Akiko NATIONAL INSTITUTE OF INFECTIOUS DISEASES, ORALSCIENCE, RESEARCHER (50291125)
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| Project Period (FY) |
1999 – 2001
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| Keywords | Anti-microbial Peptide / saliva / defensin / strentococci / peptide vaccine / dental caries / Streptococcus mutans / vaccine |
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| Research Abstract |
This study has a long-term goal of getting the elucidation of the host defense mechanisms against infection in the oral cavity. We studied it about the antibacterial factor of saliva, expression of the mainly antibacterial peptide (the peptide having antibacterial activity). In a research period, We performed a exhaustive search of the newly antibacterial protein of saliva, analysis of the expression adjustment of the antibacterial protein.
We performed the identification of the molecules which developed in saliva sequentially in last year at a nucleic acid level. For materials, we used cell strain derived from useful Macaca fascicularis salivary glands mRNA, mRNA derived from a human salivary glands, a human salivary glands as human model system. We prepared mRNA than three kinds of large salivary glands (a parotoid, a submaxillary gland, a sublingual gland) and offered the Macaca fascicularis salivary glands for an experiment to compare the expression of molecules in each salivary glands.
The cDNA composition from mRNA, RT-PCR, the decision of the DNA sequence using ordinary methods, and we identified plural molecular species of the defensin family, molecular species of histatin family, LL37 in this study. Furthermore, for the identification and analysis at the protein level, we made composition of the peptide and the antibody.
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[Journal Article] Effects of human interleukin-18 and interleukin-12 treatment on human lymphocyte engraftment in NOD-scid mouse.2002
Author(s)
Senpuku H, Asano T, Matin K, Salam MA, Tsuha Y, Horibata S, Shimazu Y, Soeno Y, Aoba T, Sata T, Hanada N, Honda M.
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Journal Title
Immunology 107
Pages: 232-242
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Clinical study of mutans streptococci using 3DS and monoclonal antibodies.2001
Author(s)
Takeuchi H, Fukushima K, Senpuku H, Nomura Y, Kaneko N, Yano A, Morita E, Imai S, Nisizawa T, Kono Y, Ikemi T, Toyoshima Y, Hanada N.
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Journal Title
Jpn J Infect Dis 54
Pages: 34-36
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Controlling opportunistic pathogens in the oral cavity of preschool children by the use of 3DS.2001
Author(s)
Nomura Y, Senpuku H, Tsuge S, Hayashi M, Sasaki A, Tamura H, Ida H, Yoshikawa E, Nishikawara F, Kawamura S, Kokubo K, Hanada N.
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Journal Title
Jpn J Infect Dis 54
Pages: 199-200
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] New dental drug delivery system for removing mutans streptococci from the oral cavity : effect on oral microbial flora.1999
Author(s)
Takeuchi H, Senpuku H, Matin K, Kaneko N, Yusa N, Yoshikawa E, Ida H, Imai S, Nisizawa T, Abei Y, Kono Y, Ikemi T, Toyoshima Y, Fukushima K, Hanada N.
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Journal Title
Jpn J Infect Dis. 53
Pages: 211-212.
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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