2001 Fiscal Year Final Research Report Summary
Mechanism of GAGA factor-dependent chromatin remodeling
| Project/Area Number |
11480204
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | National Institute of Genetics |
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Principal Investigator |
HIROSE Susumu National Institute of Genetics, Department of Development Genetics, Professor, 個体遺伝研究系, 教授 (90022730)
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| Co-Investigator(Kenkyū-buntansha) |
UEDA Hitoshi National Institute of Genetics, Department of Development Genetics, Associate Professor, 個体遺伝研究系, 助教授 (60201349)
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| Project Period (FY) |
1999 – 2001
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| Keywords | Drosophila / GAGA factor / chromatic / nucleosome / gene expression |
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| Research Abstract |
Drosophila GAGA factor directs chromatin remodeling to its binding sites. To elucidate the mechanism underlying the site-specific chromatin remodeling, we searched for GAGA factor-associated proteins. We identified two GAGA factor-associated proteins p93 and p130, and analyzed them. The results obtained showed that p93 and p130 form a heterodimer, bind to nucleosome, and facilitate chromatin remodeling. We also found that GAGA factor, p93 and p130 colocalyze to active chromatin on polytene chromosomes. Moreover, genetic interaction between Trithorax-like and p130 implicates GAGA factor-p93-p130 complex in epigenetic gene expression.
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