2001 Fiscal Year Final Research Report Summary
MOLECULAR CELL BIOLOGICAL SYUDY OF A NOVEL NEUROTROPHIC PROTEIN, PCTF35
| Project/Area Number |
11480226
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Osaka University |
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Principal Investigator |
UCHIYAMA Yasuo Osaka University Graduate School of Medicine, Department of Cell Biology and Neuroscience, Professor, 医学系研究科, 教授 (10049091)
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| Co-Investigator(Kenkyū-buntansha) |
ISHIHARA Kyoko Osaka University Graduate School of Medicine, Department of Cell Biology and Neuroscience, Assistant Professor, 医学系研究科, 助手 (30303944)
OHSAWA Yoshiyuki Osaka University Graduate School of Medicine, Department of Cell Biology and Neuroscience, Assistant Professor, 医学系研究科, 助手 (30273642)
WAGURI Satoshi Osaka University Graduate School of Medicine, Department of Cell Biology and Neuroscience, Associate Professor, 医学系研究科, 助教授 (30244908)
KAMETAKA Satoshi Osaka University Graduate School of Medicine, Department of Cell Biology and Neuroscience, Assistant Professor, 医学系研究科, 助手 (10303950)
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| Project Period (FY) |
1999 – 2001
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| Keywords | PCTF35 / Brain injuries / Astrocytes / Glyosis / TGF-b1 / Primary culture / Induction / Secretion |
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| Research Abstract |
To understand in vivo functions of PCTF35, we performed the following experiments using models of brain injuries and primary cultured astrocytes.
1) Expression of PCTF35 following brain injuries: For this we prepared the following two animals; (1) Hypoxia-ischemia (H-1) injury: new born rats at P7 were anesthetized and the left common carotid artery was ligated. The animals were returned to the darn for 1 hr and then placed in containers, which were perfused with mixed gases of 8% oxygen and 92% nitrogen and kept at 37℃, for 90 min. They were then returned to the dam until used. (2) Penetrating brain injury: Adult rats (8 weeks of age) were anesthetized and an incision with a 2mm depth and 4mm length was made in the temporal lobe after part of the temporal bone was opened. These animals were perfused from the heart with 4% paraformaldehy, de 3,4,5 and 7 days after the operation and brain tissues were processed for immunohistochemistry. At 3 days, GFAP-immunopositive astrocytes expanded their process and invaded into injured areas, while astogliosis occurred in the areas at 7 days. By double immunostaining, immunoreactivity for PCTF35 from co-localized in the processed of these astrocytes with that for GFAP.
2) Expression and secretion of PCTF35 from primary cultured astrocytes: Since PCTF35 was induced in astrocytes appearing in injured areas of brains, we separated astrocytes from brains of rats at P2. PCTF35 was found in the cultured medium of primary astrocytes by Western blot and the amount of the protein was significantly increased in the medium when TGF-β1 was added in cultures. These lines of evidence suggest that PCTF35 is induced in astrocytes of rat brains after H-I and penetrating injuries and this induction is enhanced by TGF-β1.
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[Publications] Shibata, M., Koike, M., Waguri, S., Zhang, G., Koga, T., Uchiyama, Y.: "Cathepsin D is specifically inhibited by deoxyribonucleic acids"FEBS Letters. (in press).
Description
「研究成果報告書概要(和文)」より
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[Publications] Takahashi, K., Takeuchi, J., Takahashi, T., Miyauchi, S., Horie, K., Uchiyama, Y.: "Effects of sodium hyaluronate on epithelial healing of the vesical mucosa and vesical fibrosis in rabbits with acetic acid induced cystitis"J. Urology. 166. 710-713 (2001)
Description
「研究成果報告書概要(和文)」より
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[Publications] Waguri, S., Kohmura, M., Kanamori, S., Watanabe, T., Ohsawa, Y., Koike, M., Tomiyama, Y., Wakasugi, M., Kominami, E., Uchiyama, Y.: "Different distribution patterns of the two mannose 6-phosphate receptors in rat liver"J. Histochem. Cytochem.. 49. 1397-1405 (2001)
Description
「研究成果報告書概要(和文)」より
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[Publications] Nakanishi, H., Zang, J., Koike, M., Nishioku, T., Okamoto, Y., Kominami, E., Figura, K., Peters, C., Yamamoto, K., Sartig, P., Uchiyama, Y.: "Involvement of nitric oxide release from microglia/macrophages in pathological changes of cathepsin D-deficient mice"J. Neurosci.. 21. 7526-7533 (2001)
Description
「研究成果報告書概要(和文)」より
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[Publications] Yaginuma, H., Shiraiwa, N., Shimada, T., Nishiyama, K., Hong, J., Wang, S., Momoi, M., Uchiyama, Y., Oppenheim, R.: "Caspase activity is involved in, but is dispensable for, early motoneuron death in the chick embryo cervical spinal cord"Mol. Cell. Neurosci.. 18. 168-182 (2001)
Description
「研究成果報告書概要(和文)」より
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[Publications] Shibata, M., Koike, M., Waguri, S., Zhang, G., Koga, T., Uchiyama, Y.: "Cathepsin D is specifically inhibited by deoxyribonucleic acids"FEBS Letters. (in press).
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Takahashi, K., Takeuchi, J., Takahashi, T., Miyauchi, S., Horie, K., Uchiyama, Y.: "Effects of sodium hyaluronate on epithelial healing of the vesical mucosa and vesical fibrosis in rabbits with acetic acid induced cystitis"J. Urology. 166. 710-713 (2001)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Waguri, S., Kohmura, M., Kanamori, S., Watanabe, T., Ohsawa, Y., Koike, M., Tomiyama, Y., Wakasugi, M., Kominami, E., Uchiyama, Y.: "Different distribution patterns of the two mannose 6-phosphale receptors in rat liver"J. Histochem. Cytochem.. 49. 1397-1405 (2001)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Nakanishi, H., Zang, J., Koike, M., Nishioku, T., Okamoto, Y., Kominami, E., Figura, K.v., Peters, C., Yamamoto, K., Sattig, P., Uchiyama, Y.: "Involvement of nitric oxide release from microglia macrophages in pathological changes of cathepsin D- deficient mice"J. Neurosci. 21. 7526-7533 (2001)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Yaginuma, H., Shiraiwa, N., Shimada, T., Nishiyama, K., Hong, J., Wang, S., Momoi, M., Uchiyama, Y., Oppenheim, R.: "Caspase activity is involved in, but is dispensable for, early motoneuron death in the chick embryo cervical spinal cord. Mol. Cell"Neurosci. 18. 168-182 (2001)
Description
「研究成果報告書概要(欧文)」より
