2000 Fiscal Year Annual Research Report

生体レーザアブレーションのナノ秒時間ゲート分光によるカルシウム検出と医療応用

Research Project

Project/Area Number	11480256
Research Institution	Osaka University
Principal Investigator	春名正光大阪大学, 医学部, 教授 (20029333)
Co-Investigator(Kenkyū-buntansha)	近江雅人大阪大学, 医学部, 助手 (60273645) 森本茂人大阪大学, 大学院・医学系研究科, 助教授 (20150336)
Keywords	生体レーザアブレーション / ナノ秒時間ゲート分光 / Ca検出モニター / 光コヒーレンストモグラフィ / 高分解能OCT / マイクロ光診断・治療
Research Abstract	前年度に提案・装置化した発光プルームのナノ秒時間ゲート分光法を用いて、ラットを用いた系統的なCa検出実験を行い、本手法が日常的なCa摂取モニターとして利用できることを示唆した。また、石灰化した病変部の診断・除去を含む新たなマイクロ光診断・治療技術を提案・検討した。 (1)低Ca飼料を与えたラット群および通常飼料の対照ラット群において、各群のラット爪のCa検出を継続的に行った。実験は4週齢から開始し、10週齢以降で低Caラット群の爪Caピーク強度が有意に低下することを見い出した。 (2)さらに、4週齢ラットの卵巣を摘出し、毎週その爪Caピーク強度を測定した。対照ラット群に対して、卵巣摘出群では爪Caピークの低下が認められている。 (3)上記の実験から、ナノ秒時間ゲート分光法は日常的なCa摂取モニターとして利用できる見通しを得た。 (4)レーザアブレーションと光コヒーレンストモグラフィ(OCT)を融合して、新たに生体表面の厚さ方向のCa分布測定、およびμmオーダーでの病変部の除去あるいは生体加工・治療が行なえることを提案した。これらをマイクロ光診断・治療と呼ぶ。 (5)上記の提案の基礎実験として、ラット大腿骨の半径方向のCa分布を測定した。 (6)新たなマイクロ光診断・治療を目指して、1.3μmの超高分解能でレーザアブレーションされた生体表面のOCT断層像の取得に成功した。

Research Products
(15 results)

All Other

All Publications (15 results)

[Publications] Masamitsu Haruna: "Detection of phase transition of a biological membrane by precise refractive-index measurement based on the low coherence interferometry"Proc.SPIE, Progress in Biomed.Opt.& Imaging. Vol.3915. 188-193 (2000)
[Publications] Masamitsu Haruna: "Calcium detection of human hair and nail by the nanosecond time-gated spectroscopy of laser-ablation plume"Proc.SPIE, Progress in Biomed.Opt.& Imaging. Vol.3917. 87-92 (2000)
[Publications] Masamitsu Haruna: "Optical reflection imaging for display of 2-D refractive index distribution along the geometrical thickness"OSA Biomed.Topical Meetings, Tech.Dig.. MB5. 194-196 (2000)
[Publications] Masato Ohmi: "Nanosecond time-gated spectroscopy of laser-ablation plume of human hair to detect calcium for potential diagnoses"Optical Review. Vol.7,No.4. 353-357 (2000)
[Publications] Koji Yoden: "An approach to optical reflection tomography along the geometrical thickness"Optical Review. Vol.7,No.5. 402-405 (2000)
[Publications] Hideki Maruyama: "Simultaneous measurement of refractive index and thickness by low coherence interferometry considering chromatic dispersion of index"Optical Review. Vol.7,No.5. 468-472 (2000)
[Publications] Masato Ohmi: "In vitro simultaneous measurement of refractive index and thickness by low coherence interferometry"IEEE Trans.Biomed.Eng.. Vol.47,No.9. 1266-1270 (2000)
[Publications] Shogo Inoue: "Ultra low coherence interferometry for determination of refractive index and thickness of transparent plate"14th Int'l Conf.Opt.Fiber Sensors(OFS-14). P2-21. 468-471 (2000)
[Publications] Masato Ohmi: "Optical reflection imaging along the geometrical thickness by combination of coherence-gate and confocal imagings"14th Int'l Conf.Opt.Fiber Sensors(OFS-14). Th1-3. 288-291 (2000)
[Publications] 春名正光: "光コヒーレンストモグラフィ(OCT)-原理と開発の現状-"臨床消化器内科. Vol.16,No.1. 131-133 (2001)
[Publications] 春名正光: "生体のマイクロ光診断と実サイズ光トモグラフィ"O plus E. Vol.23,No.1. 86-89 (2001)
[Publications] Masamitsu Haruna: "In situ tomographic observation of tissue surface during laser ablation"Proc.SPIE, Progress in Biomed.Opt.& Imaging. Vol.4257(to be published). (2001)
[Publications] Masato Ohmi: "Optical reflection tomography along the geometrical thickness"Proc.SPIE, Progress in Biomed.Opt.& Imaging. Vol.4251(to be published). (2001)
[Publications] Ryuh Konoshita: "Measurement of calcium of biological tissues along the depth by laser ablation"Conf.Lasers & Electro-Optics(CLEO/PR2001). (to be published). (2001)
[Publications] Masato Ohmi: "Optical reflection tomography along the geometrical thickness toward in vivo imaging of biological tissue"Conf.Lasers & Electro-Optics(CLEO/PR2001). (to be published). (2001)

2000 Fiscal Year Annual Research Report

生体レーザアブレーションのナノ秒時間ゲート分光によるカルシウム検出と医療応用

Principal Investigator

春名 正光 大阪大学, 医学部, 教授 (20029333)

Research Products

[Publications] Masamitsu Haruna: "Detection of phase transition of a biological membrane by precise refractive-index measurement based on the low coherence interferometry"Proc.SPIE, Progress in Biomed.Opt.& Imaging. Vol.3915. 188-193 (2000)

[Publications] Masamitsu Haruna: "Calcium detection of human hair and nail by the nanosecond time-gated spectroscopy of laser-ablation plume"Proc.SPIE, Progress in Biomed.Opt.& Imaging. Vol.3917. 87-92 (2000)

[Publications] Masamitsu Haruna: "Optical reflection imaging for display of 2-D refractive index distribution along the geometrical thickness"OSA Biomed.Topical Meetings, Tech.Dig.. MB5. 194-196 (2000)

[Publications] Masato Ohmi: "Nanosecond time-gated spectroscopy of laser-ablation plume of human hair to detect calcium for potential diagnoses"Optical Review. Vol.7,No.4. 353-357 (2000)

[Publications] Koji Yoden: "An approach to optical reflection tomography along the geometrical thickness"Optical Review. Vol.7,No.5. 402-405 (2000)

[Publications] Hideki Maruyama: "Simultaneous measurement of refractive index and thickness by low coherence interferometry considering chromatic dispersion of index"Optical Review. Vol.7,No.5. 468-472 (2000)

[Publications] Masato Ohmi: "In vitro simultaneous measurement of refractive index and thickness by low coherence interferometry"IEEE Trans.Biomed.Eng.. Vol.47,No.9. 1266-1270 (2000)

[Publications] Shogo Inoue: "Ultra low coherence interferometry for determination of refractive index and thickness of transparent plate"14th Int'l Conf.Opt.Fiber Sensors(OFS-14). P2-21. 468-471 (2000)

[Publications] Masato Ohmi: "Optical reflection imaging along the geometrical thickness by combination of coherence-gate and confocal imagings"14th Int'l Conf.Opt.Fiber Sensors(OFS-14). Th1-3. 288-291 (2000)

[Publications] 春名正光: "光コヒーレンストモグラフィ(OCT)-原理と開発の現状-"臨床消化器内科. Vol.16,No.1. 131-133 (2001)

[Publications] 春名正光: "生体のマイクロ光診断と実サイズ光トモグラフィ"O plus E. Vol.23,No.1. 86-89 (2001)

[Publications] Masamitsu Haruna: "In situ tomographic observation of tissue surface during laser ablation"Proc.SPIE, Progress in Biomed.Opt.& Imaging. Vol.4257(to be published). (2001)

[Publications] Masato Ohmi: "Optical reflection tomography along the geometrical thickness"Proc.SPIE, Progress in Biomed.Opt.& Imaging. Vol.4251(to be published). (2001)

[Publications] Ryuh Konoshita: "Measurement of calcium of biological tissues along the depth by laser ablation"Conf.Lasers & Electro-Optics(CLEO/PR2001). (to be published). (2001)

[Publications] Masato Ohmi: "Optical reflection tomography along the geometrical thickness toward in vivo imaging of biological tissue"Conf.Lasers & Electro-Optics(CLEO/PR2001). (to be published). (2001)

春名正光大阪大学, 医学部, 教授 (20029333)