2001 Fiscal Year Final Research Report Summary
Molecular manipulation and application of thermophilic cyanobacteria
| Project/Area Number |
11554035
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
植物生理
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| Research Institution | The University of Tokyo |
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Principal Investigator |
IKEUCHI Masahiko The University of Tokyo, Graduate School of Arts and Sciences, Associate Professor, 大学院・総合文化研究科, 助教授 (20159601)
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| Co-Investigator(Kenkyū-buntansha) |
SUGIURA Miwa Osaka Prefecture University, Faculty of Agriculture, Research Assistant, 農学部, 助手 (80312255)
HIRANO Masahiko Toray Research Institute, Chief Scientist, 生物研究部, 次長(研究職)
JIAN-REN Shen RIKEN Harima Institute, Photosynthesis Research Laboratory, Senior Researcher, 光合成研究室, 研究員 (60261161)
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| Project Period (FY) |
1999 – 2001
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| Keywords | cyanobacteria / blue-green algae / photosynthetic production / thermophile / gene manipulation / gene expression / photosystem II / crystallization |
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| Research Abstract |
A transformation-competent clone of the thermophilic cyanobacterium Thermosynechococcus elongatus strain BP-1 was established. In collaboration with S. Tabata (Kazusa DNA Res. Inst.), we determined the complete nucleotide sequence.
A gene for a novel flavin-binding protein was cloned from T. elongatus and expressed in E. coli.
Phycocyanin was isolated from T. elongatus and crystalled. X-ray diffraction data at 2.0 A resolution was obtained.
Gene disruption mutants were created for psbI, psbT, psbV and psbK in T. elongatus. Photosystem II complexes were isolated from these mutants.
A number of site-directed mutants were constructed for the extrinsic 33 kDa protein of T. elongatus and were analyzed functionally.
O_2-evolving Photosystem II complex was isolated from wild type of T. vulcanus and crystallized. The 3D
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Research Products
(14 results)
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[Publications] Ohmori, M., M. Ikeuchi, N. Sato, P. Wolk, T. Kaneko, T. Ogawa, M. Kanehisa, S. Goto, S. Kawashima, S. Okamoto, H. Yoshimura, H. Katoh, T. Fujisawa, S. Ehira. A. Kamei, S. Yoshihara, R. Narikawa, and S.Tabata: "Characterization of genes encoding multi-domain proteins in the genome of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. Strain PCC 7120"DNA Res. 8. 271-284 (2001)
Description
「研究成果報告書概要(欧文)」より
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