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2000 Fiscal Year Final Research Report Summary

Establishment of an animal model for human cellular againg

Research Project

Project/Area Number 11554038
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section展開研究
Research Field 人類学(含生理人類学)
Research InstitutionUniversity of Tokyo

Principal Investigator

ISHIDA Takafumi  Univ.Tokyo, Grad.School Sci., Assoc.Prof, 大学院・理学系研究科, 助教授 (20184533)

Co-Investigator(Kenkyū-buntansha) SUZUKI Juri  Kyoto Univ., Primate Res.Inst Res.Assoc., 霊長類研究所, 助手 (10175408)
Project Period (FY) 1999 – 2000
Keywordsmacaque / cellular aging / in vitro / human / model
Research Abstract

To clarify the pattern of macaque cellular aging, a total of 40 cultures of Japanese macaque (Macaca fuscata), long-tailed macaque (Macaca fascicularis), bonnet monkey (Macaca radiata) and rhesus macaque (Macacamulatta) were carried out. Adherent cells were isolated from skin, kidney and lung, cultured in RPMI 1640 medium supplemented with 10% FBS, and subcultured until they terminated cell division. In 36 cultures, cells underwent senescence showing lager cell size and decreased saturation density, and then terminated cell division (Mortality stage 1 (M1)) at 10-20 Population Doubling Levels (PDLs). Whereas cells in four cultures first exhibited senescent morphology at 15-25 PDLs, but continued cell division through M1 up to 40-100 PDLs and then came into crisis (Mortality stage 2 (M2)). Cells in one culture of Japanese macaque do not terminate cell division (>150 PDLs) and showed several characteristics of transformed cells, such as the anchorage independent growth and loss of contact inhibition. Shortening of telomeres by PDLs and the absence of telomerase activity were identified in all of tested including the transformed one. Macaque cells showed an intermediate pattern of in vitro aging between human and rodent cells, whereas, in terms of telomerase activity, they are relatively close to human cells. It is thus suggested that the macaques serve as animal models for human cellular aging and that they provide us with a key to study possible mechanisms which play roles in the critical stages (M1 and M2) in cellular aging and transformation.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Suzuki,J.,Ishida,T. et al.: "Plasma IGF-1 levels in the great and lesser apes."Proc.XVIIIth Congr.Int.Primatol.Soc.. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suzuki,J.and Ishida,T.: "Age changes in plasma IGF-1 concentration in free-ranging Japanese macaques (Macaca fuscata)."J.Med.Primatol.. 30(in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suzuki,J. et al.: "Time series analysis of plasma insulin-like growth factor-I and gonadal steroids in adolescent Japanese macaques (Macaca fuscata)."J.Reprod.Dev.. 46. 157-166 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suzuki,J. et al.: "Autoimmune hemolytic anemia (AIHA) in an infant rhesus macaque (Macaca mulatto)."J.Med.Primatol.. 29. 88-94 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shimizu,Y. et al.: "Sero-and molecular-typing of Duffy blood group in Southeast Asians and Oceanians."Hum.Biol.. 72. 511-518 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Pranomo,ZAD. et al.: "A novel cryptic exon in intron 2 of the dystrophin gene evolved from intron by creating consensus sequences for splicing in different stages of the anthropoid evolution"Biochem.Biophys.Res.Commun.. 267. 321-328 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suzuki, J., Ishida, T., Ohkura, S., Udono, T., Hayasaka, I.and Araithamkul, V.: "Plasma IGF-1 levels in the great and lesser apes."Proc.XVIIIth Congr.Int.Primatol.Soc., Adelaide. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzuki, J.and Ishida, T.: "Age changes in plasma IGF-1 concentration in free-ranging Japanese macaques (Macacafuscata)."Journal of Medical Primatology. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzuki, J., Ohkura, S., Hayakawa, S.and Hamada, Y.: "Time series analysis of plasma insulin-like growth factor-I and gonadal steroids in adolescent Japanese macaques (Macacafuscata)."Journal of Reproduction and Development. 46. 157-166 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzuki, J., Miwa, N., Terao, K.and Nakayama, H.: "Autoimmune hemolytic anemia (AIHA) in an infant rhesus macaque (Macacamulatta)."Journal of Medical Primatology. 29. 88-94 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimizu, Y., Ao, H., Soemantri, Ag., Tiwawech, D., Settheetham-Ishida, W., Kayame, O.W., Nishioka, T.and Ishida, T.: "Sero-and molecular typing of Duffy blood group in Southeast Asians and Oceaniams."Human Biology. 72. 511-518 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Pranomo ZAD, Takeshima, Y., Surono, A., Ishida, T.and Matsuo, M.: "A novel cryptic exon in intron 2 of the dystrophin gene evolved from intron by creating consensus sequences for splicing in different stages of the anthropoid evolution."Biochem.Biophys.Res.Commun.. 267. 321-328 (2000)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2002-03-26  

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