2000 Fiscal Year Final Research Report Summary
Development of a novel biotechnology for decomposing of plastic using PET (polyethylene terephthalate)-decomposing microorganisms
| Project/Area Number |
11555249
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
高分子合成
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| Research Institution | Kyoto Institute of Technology |
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Principal Investigator |
KIMURA Yoshiharu Kyoto Institute of Technology, Faculty of Textile Science, Professor, 繊維学部, 教授 (10132276)
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| Co-Investigator(Kenkyū-buntansha) |
ODA Kohei Kyoto Institute of Technology, Department of Applied Biology, Professor, 繊維学部, 教授 (50081584)
MIYAMOTO Masatoshi Kyoto Institute of Technology, Faculty of Textile Science, Associate Professor, 繊維学部, 助教授 (70149524)
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| Project Period (FY) |
1999 – 2000
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| Keywords | polyethylene terephthalate / terephthalate / plastic / PET-decomposing microorganism / polyester / bio-degradation |
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| Research Abstract |
The results in this research are as follows.
1.Screening, isolation, and identification of PET-decomposing microorganisms : We have succeeded in getting six strains, which could decompose the non-crystalline PET fibers. Some of them were identified as Trichosporon sp.No.85, Sphingomonas paucimobilis No.77-2, and Arthrobacter sp.No.97-2, respectively.
2.Purification of PET-decomposing enzyme from Arthrobacter sp.No, 97-2 : A 35-kDa protein was purified as a candidate for PET-decomposing enzymes from the culture filtrate of Arthrobacter sp.No.97-2. The amino-terminal sequence of the purified enzyme showed high similarity with that of flagellin (identity ; 63% and similarity ; 75%). We could not elucidate enzymatic properties, because of low specific activity.
3.Development of new assay methods : Observations of the surface of PET fiber by a scanning electron microscopy and measurements of tensile strength of PET fiber have been used for assay of PET-decomposing activities. However, these assay methods take a lot of time, and also their data are not accurate. To improve assay methods for PET-decomposing enzymes, measurements of viscosity, gel permeation chromatography, and NMR spectroscopy were introduced. Four kinds of substrates were used : PET fiber, PET film, PET oligomer, and OEST (oligo (ethylene succinate-co-terephthalate). These assay methods turned out to be useful for assay of PET-decomposing activities.
Some of our researches were not carried. The reasons are (1) it was very difficult to keep such PET-decomposing strains with high activities. These enzymes might be coded on plasmids. (2) There were no any adequate assay methods, which are reliable.
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