2001 Fiscal Year Final Research Report Summary
Development of microsatellite markers for radish breeding
| Project/Area Number |
11556002
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Breeding science
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| Research Institution | University of Tsukuba |
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Principal Investigator |
FUJIMURA Tatsuhito University of Tsukuba, Institute of Agric. And Forest Enginering, Professor, 農林工学系, 教授 (70292513)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUURA Seiji Tohoku seed, Co.Ltd,Research section Plant Breeder, 育種部, 主任研究員
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| Project Period (FY) |
1999 – 2001
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| Keywords | genome / microsatellites / genetic map |
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| Research Abstract |
This research was planed to develop the new method for constructing the microsatellite markers of radish which will form a back born of radish genome map. Those makers will facilitate radish breeding. Radish DNA was digested with two kind of restriction enzymes and then ligated with two kinds of adapters specific to each restriction sites. These chimeric DNA fragments were preliminary amplified by PCR, and then hybridized to DNA oligomer composed of microsatellite motif on a nylon membrane, DNA fragments hybridized were eluted from a membrane, and amplified with selective PCR printers with one to two base 3' extensions. Reduced number of PCR products were separated easily on an acryl amide gel electrophoresis. These separated PCR products were sequence directly and finally constructed twenty microsatellite markers.
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