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2002 Fiscal Year Final Research Report Summary

Development of Biomonitoring Technology for Environmental Endocrine Disruptors

Research Project

Project/Area Number 11556016
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 応用微生物学・応用生物化学
Research InstitutionKobe University

Principal Investigator

OHKAWA Hideo  Research Center for Environmental Genomics, Professor, 遺伝子実験センター, 教授 (90233048)

Co-Investigator(Kenkyū-buntansha) MIYAKE Shiro  Horiba Biotechnology Inc., Research Manager, 開発・営業部長(研究職)
KAWATA Mitsuyasu  Otsuka Chemical Co., Ltd., Naruto Research Center, Research Scientist, 鳴門研究所, 主任研究員
KATAGIRI Masanao  Osaka Kyoiku University, Division of Natural Science, Associate Professor, 教育学部, 助教授 (00185802)
Project Period (FY) 1999 – 2002
KeywordsELISA / monoclonal antibody / scFv / endocrine disruptor / simazine / bisphenol A / alachlor / nonylphenol
Research Abstract

Contamination of the environment and agricultural products with environmental endocrine disruptors is a serious global problem. These chemicals were found in the environment and agricultural products at nano-level concentrations and suspected of affecting ecosystems and human health. Therefore, it is requested to develop novel technologies to monitor contaminations by nano-levels of environmental endocrine disruptors since most conventional technologies are high cost, labor intensive and taking time to obtain results, and do not work effectively to monitor nano-level contaminants.
(1) We prepared monoclonal antibodies specific to the endocrine disruptors bisphenol A, and nonylphenol, and the herbicides alachlor and simazine, and then developed enzyme-linked immunosorbent assay (ELISA) based on the monoclonal antibodies. The ELISA methods developed were highly sensitive, convenient and low-cost as compared with the conventional analytical methods.
(2) We also developed recombinant scFv antibodies based on the monoclonal antibodies by genetic engineering and used for preparation of affinity columns for clear-up of the extracts of samples of soil and agricultural products.
(3) It was found that the combined use the novel ELISA method with the affinity column produced highly efficient monitoring data for environmental endocrine disruptors in a various samples.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] M.Nakata, A.Fukushima, H.Ohkawa: "A monoclonal antibody-based ELISA for the analysis of the insecticide flucythrinate in environmental and crop samples"Pest.Manag, Sci.. 57. 269-277 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] J.Eto, Y.Suzuki, H.Ohkawa I.Yamaguchi: "Preparation of a Functional Single-Chain Antibody Against Chlorpropham"J.Pesticide Sci.. 27. 383-386 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 西 甲介, 大川秀郎: "環境負荷化学物質のバイオモニタリング法"日本農薬学会誌. 27. 157-165 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Nishi, Y.Imajuku, M.Nakata, K.Ohde, S.Miyake, K.Morimune, M.Kawata, H.Ohkawa: "Molecular Characteristics of the Monoclonal and Recombinant Antibodies Specific to the Insecticide Malathion"J.Pesticide Sci.. (印刷中). (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Nishi, M.Takai, K.Morimune, H.Ohkawa: "Molecular and Immunochemical Characteristics of Monoclonal and Recombinant Antibodies Specific to Bisphenol A"Bioscience, Biotechnology and Biochemistry. (印刷中). (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Miyake, Y.Ishii, Y Yamaguchi, K.Ohde, M.Motoki, M.Kawata, S.Ito, Y.Yuasa, H.Ohkawa: "Simple Determination of Herbicides in Rice Paddy Water by Immunoassay. Pesticide Science : Environmental Fate and Effects of Pesticides"ACS Symposium Series No.853, Eds by J.R.Coats and H.Yamamoto, American Chemical Society, Washington, D.C., USA. (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大川秀郎, 西 甲介: "バイオアッセイ的手法の利用、「環境技術・装置大事典」"産業調査会. 1492 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M. Nakata, A. Fukushima, and H. Ohkawa: "A monoclonal antibody-based ELIZA for the analysis of the insecticide flucythrinate in environmental and crop samples"Pest. Manag. Sci.. 57. 269-277 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] J. Eto, Y. Suzuki, H. Ohkawa, and I. Yamazaki: "Preparation of a Functional Single-Chain Antibody Against Chlorpropham"J. Pesticide Sci.. 27. 383-386 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K. Nishi, and H. Ohkawa: "Biomonitoring Technology for Environmental Chemicals"J. Pesticide Sci.. 27. 157-165 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K. Nishi, Y. Imajuku, M. Nakata, K. Ohde, S. Miyake, K. Morimune, M. Kawata, and H. Ohkawa: "Molecular Characteristics of the Monoclonal and Recombinant Antibodies Specific to the Insecticide Malathion"J. Pesticide Sci.. accepted. (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K. Nishi, M. Takai, K. Morimune, and H. Ohkawa: "Molecular and Immunochemical Characteristics of Monoclonal and Recombinant Antibodies Specific to Bisphenol A"Bioscience, Biotechnology and Biochemistry. accepted. (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S. Miyake, Y. Ishii, Y. Yamazaki, K. Ohde, M. Motoki, M. Kawata, S. Ito, Y. Yuasa, and H. Ohkawa, Eds by J.R. Coats, and H. Yamamoto: "Simple Determination of Herbicides in Rice Paddy Water by Immunoassay. in: Pesticide Science: Environmental Fate and Effects of Pesticides. ACS Symposium Series No. 853"American Chemical Society. (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Ohkawa, and K. Nishi: "Use of Bioassay Technology in: Dictionary of Environmental Technologies and Equipments"Sangyo-chosa-kai. (2003)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2004-04-14  

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