2002 Fiscal Year Final Research Report Summary
Development of Biomonitoring Technology for Environmental Endocrine Disruptors
| Project/Area Number |
11556016
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
応用微生物学・応用生物化学
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| Research Institution | Kobe University |
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Principal Investigator |
OHKAWA Hideo Research Center for Environmental Genomics, Professor, 遺伝子実験センター, 教授 (90233048)
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| Co-Investigator(Kenkyū-buntansha) |
MIYAKE Shiro Horiba Biotechnology Inc., Research Manager, 開発・営業部長(研究職)
KAWATA Mitsuyasu Otsuka Chemical Co., Ltd., Naruto Research Center, Research Scientist, 鳴門研究所, 主任研究員
KATAGIRI Masanao Osaka Kyoiku University, Division of Natural Science, Associate Professor, 教育学部, 助教授 (00185802)
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| Project Period (FY) |
1999 – 2002
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| Keywords | ELISA / monoclonal antibody / scFv / endocrine disruptor / simazine / bisphenol A / alachlor / nonylphenol |
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| Research Abstract |
Contamination of the environment and agricultural products with environmental endocrine disruptors is a serious global problem. These chemicals were found in the environment and agricultural products at nano-level concentrations and suspected of affecting ecosystems and human health. Therefore, it is requested to develop novel technologies to monitor contaminations by nano-levels of environmental endocrine disruptors since most conventional technologies are high cost, labor intensive and taking time to obtain results, and do not work effectively to monitor nano-level contaminants.
(1) We prepared monoclonal antibodies specific to the endocrine disruptors bisphenol A, and nonylphenol, and the herbicides alachlor and simazine, and then developed enzyme-linked immunosorbent assay (ELISA) based on the monoclonal antibodies. The ELISA methods developed were highly sensitive, convenient and low-cost as compared with the conventional analytical methods.
(2) We also developed recombinant scFv antibodies based on the monoclonal antibodies by genetic engineering and used for preparation of affinity columns for clear-up of the extracts of samples of soil and agricultural products.
(3) It was found that the combined use the novel ELISA method with the affinity column produced highly efficient monitoring data for environmental endocrine disruptors in a various samples.
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[Publications] S.Miyake, Y.Ishii, Y Yamaguchi, K.Ohde, M.Motoki, M.Kawata, S.Ito, Y.Yuasa, H.Ohkawa: "Simple Determination of Herbicides in Rice Paddy Water by Immunoassay. Pesticide Science : Environmental Fate and Effects of Pesticides"ACS Symposium Series No.853, Eds by J.R.Coats and H.Yamamoto, American Chemical Society, Washington, D.C., USA. (2003)
Description
「研究成果報告書概要(和文)」より
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[Publications] S. Miyake, Y. Ishii, Y. Yamazaki, K. Ohde, M. Motoki, M. Kawata, S. Ito, Y. Yuasa, and H. Ohkawa, Eds by J.R. Coats, and H. Yamamoto: "Simple Determination of Herbicides in Rice Paddy Water by Immunoassay. in: Pesticide Science: Environmental Fate and Effects of Pesticides. ACS Symposium Series No. 853"American Chemical Society. (2003)
Description
「研究成果報告書概要(欧文)」より
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