2001 Fiscal Year Final Research Report Summary
THE PRODUCTION OF THE NEW POLYAMIDE TYPE POLYMER MATERIALS FROM LIGNIN USING GENE RECOMBINATION OF MICRO ORGANISM
| Project/Area Number |
11556030
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
林産学
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| Research Institution | TOKYO UNIVERSITY OF AGRICULTURE & TECHNOLOGY |
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Principal Investigator |
KATAYAMA Yoshihiro TOKYO UNIVERSITY OF AGRICULUTURE & TECHNOLOGY, GRADUATE SCHOOL OF BIO-APPLICATIONS & SYSTEM ENGINEERING, SYSTEM ENGINEERING, 大学院・生物システム応用科学研究科, 助教授 (10214339)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIKAWA Seiji COSMO OIL CO., LTD., RESEARCH & DEVELOPMENT CENTER, MANAGER SPECIALTY CHEMICAL GROUP, 中央研究所, (研究職)グループ長
SHIGEHARA Kiyotaka TOKYO UNIVERSITY OF AGRICULUTURE & TECHNOLOGY, GRADUATE SCHOOL OF BIO-APPLICATIONS & SYSTEM ENGINEERING, SYSTEM ENGINEERING, 工学部, 教授 (60170867)
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| Project Period (FY) |
1999 – 2001
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| Keywords | liignin / biotransformation / Sptiingomoas paucimobilis SYK-6. / genelJc engineering / 2-Pyrone-4,6-dicarboxylicacid / bio-reactor / polyamidetype polymer / interfacialpolymerizatiou reaction |
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| Research Abstract |
This project has been investigating the biotransformation of lignin based on the catabolism of ligninolytic bacterium Sphing omonas paucimobilis SYK-6. 2-Pyroae-4 , 6-dicarboxylicacid (PDC) is a major and characteristic intermediate of protocatecuate meta cleavage pathway of Sphinomonas paucimobilis SYK-6 in lignin degradation. We have preparedthe new recombinant plasmid vector which was based on the Escherichia coli.-Pseudomonas shutU.5 vector PKT-230 and composed of Lig A, B genes (protocatechuate 4,4-dioxygenase gene), the modified VanA, B gene (Vanillate, Syringate demethylase gene) and Lig C gene (PDC synthase). In this project research, we have succeeded in producing PDC in the high yield (ca.90%) from the low-molecular weight lignin by the transformant of Pseodoaiowsputicla PpYllOO having the new reconbinantplasmid vector.
Since PDC has the unique molecular structure and the polymer made froin PDC is expectedto have the new function as optical material, we prepared new polyamide type polymer material by the inteifacial polymerizationreaction with hexamethylenediamine.
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[Publications] Hara, F., Masai, E., Katayama, Y., Fukuda, M.: "The 4-Oxalomesaconate Hydratase Gene, Involved in the Protocatechuate 4,5-Cleavage Pathway, is Essential to vanillate and Syringate Degradation in Sphingomonas paucimobilis SYK-6"Journal of Bacteriology. 24. 6950-6957 (2000)
Description
「研究成果報告書概要(和文)」より
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[Publications] Masai, E., Momose, K., Hara, H., Nishikawa, S., Katayama, Y., Fukuda, M.: "Genetic and Biochemical Characterization of the 4-Carboxy-2-Hydroxymuconate-6-Semialdehyde Dehydrogenase and its Role in the Protocatechuate 4,5-Cleavage Pathway in Sphingomonas paucimobilis SYK-6"Journal of Bacteriology. 23. 6651-6658 (2000)
Description
「研究成果報告書概要(和文)」より
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[Publications] Sonoki, K., Ohtsuka, Y., Ikeda, S., Masai, E., Kajita, K., Katayama, Y.: "Close Association between the Enzymes Involved in the Lignin Metabolic Pathway of Sphingomonas paucimobilis SYK-6"Journal of Wood Science. 48. 250-252 (2002)
Description
「研究成果報告書概要(和文)」より
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[Publications] Sonoki, K., Ohtsuka, Y., Ikeda, S., Masai, E., Kajita, K., Katayama, Y.: "Tetrahydrofolate Dependent Vanillate O Demethylation tightly links to One-Carbon Metabolic Pathway associated with Amino Acid Synthesis and DNA Methylation in Lignin Metabolism of Sphingomonas paucimobilis SYK-6"Journal of Wood Science. (印刷中). (2002)
Description
「研究成果報告書概要(和文)」より
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[Publications] Masai, E., Momose,K., Hara,H. Nishikawa,S., katayama,Y., Fuku da. M: "Genetic and Biochemical Characterization of the 4-Carboxy-2-Hydroxymuconate-6-Semialdehyde Dehydrogenase and Its Rolein the Protocatechuate 4,5-Cjeavage Pathway in"Journal of Bacteriology. 23. 6651-6658 (2000)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Sonoki,K., Ohtsuka, Y., Ikeda,S.,Masai, E., Kajita,K., Katayama, Y: "Close Association between the Enzymes Involved in the Lignin Metabolic Pathway of Sphingomonas paucimobilis SYK-6"Journal of Wood Science. 48. 250-252 (2002)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Sonoki,K., Ohtsuka, Y., Ikeda,S., Masai, E., Kajita,K., Katayama, Y: "Telrahydrofolate Dependent Vanillate O Demethylation tightly links to One-Carbon Metabolic Pathway associated With Amino Acid Synthesisand DNA Methylation in Lignin Metabolism of Sphingomonas paucimobilis SYK-6."Journal of Wood Science. in press. (2002)
Description
「研究成果報告書概要(欧文)」より
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