2000 Fiscal Year Final Research Report Summary
MORECULAR GENETICAL INVESTIGATION AND DNA DIAGNOSTIC SYSTEM FOR BOVINE CHONDRODYSPLASTIC DWARFISM
| Project/Area Number |
11556055
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Applied animal science
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| Research Institution | OKAYAMA UNIVERSITY |
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Principal Investigator |
KUNIEDA Tetsuo OKAYAMA UNIVERSITY, GRADUATE SCHOOL OF NATURAL SCIENCE AND TECHNOLOGY, PROFESSOR, 大学院・自然科学研究科, 教授 (80178011)
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| Co-Investigator(Kenkyū-buntansha) |
MORITOMO Yasuo KYUSYU TOKAI UNIVERSITY, FACULTY OF AGRICULTURE, ASSOCIATE PROFESSOR, 農学部, 助教授 (70148972)
OIKAWA Takuro OKAYAMA UNIVERSITY, FACULTY OF AGRICULTURE, ASSOCIATE PROFESSOR, 農学部, 助教授 (90160407)
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| Project Period (FY) |
1999 – 2000
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| Keywords | CHONDRODYSPLASA / JAPANESE BROWN CATTLE / COMPARATIVE MAPPING / HEREDITARY DISEASE / DNA DIAGNOSTIC SYSTEM / LINKAGE MAPPING / RH MAPPING |
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| Research Abstract |
The aim of the present study is to identify the gene responsible for bovine chondrodysplatic dwarfism and establish the DNA diagnosis system for the disease. A hereditary chondrodysplastic dwarfism has been reported in the particular population of Japanese Brown cattle. Affected animal shows an insufficiency of endochondral ossification at long bones of the limbs and no other associated changes. An autosomal recessive trait of inheritance has been revealed by investigating pedigrees of the affected animals.
In the present study, we mapped the locus responsible for the disease by linkage analysis using microsatellite markers and a single paternal half-sib family (n=82) of affected animals obtained from commercial herds. The linkage analysis revealed a significant linkage between the locus, referred as bcd, and marker loci on bovine Chromosome 6. The bcd locus was mapped between microsatellite markers BP7 and BM9257. A comparison of genetic maps between bovine and human indicates that the majority of bovine chromosome 6 is homologous to the segment of human chromosome 4 between 4p16.3 to 4q25. The segment involves several candidate genes including FGFR3 gene which is responsible for human achondroplasia. To determine exact relation between bovine chromosome 6 and human chromosome 4, we performed radiation hybrid mapping using the bovine hamster radiation hybrid panel. Consequently, a comprehensive radiation hybrid map of bovine chromosome 6 consisting with 33 functional genes and 32 microsatellite markers was constructed. A comparison between the radiation hybrid map of bovine chromosome 6 and human chromosome 4 revealed the region of human chromosome 4 corresponding to the region of bovine chromosome 6 in which the bcd locus is localized.
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[Publications] Noguchi, J., Kobayashi, E., Shimada, A., Kikuchi, K., Kaneko, H., Takahashi, H., Ikadai, H.and Kunieda, T.: "A locus responsible for arrest of spermatogenesis is located on rat chromosome 12."Mamm.Genome. 10. 189-190 (1999)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Yoneda, K., Moritomo, Y., Takami, M., Hirata, S., Kikukawa, Y., and Kunieda, T.: "Localization of a locus responsible for the bovine chondrodysplastic dwarfism (bcd) on chromosome 6"Mamm.Genome. 10. 597-600 (1999)
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[Publications] Nakagiri, H., Kunieda, T., Ide, H., Yoneda, K., Takami, M., and Ogawa, H.: "Allele-specific polymerase chain reaction for identifying carriers of Chediak-Higashi syndrome in Japanese black cattle."Anim.Sci.J.. 70. 372-374 (1999)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Kunieda, T., Ide, H., Nakagiri, M., Yoneda, K., Konfortov, B., and Ogawa, H.: "Localization of the locus responsible for Chediak-Higashi syndrome in cattle to bovine chromosome 28."Anim.Genet. 31. 87-90 (2000)
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[Publications] Ohba, Y., Kitagawa, H., Kitoh, K., Asahina, S., Nishimori, K., Kazuhiro, K., Kunieda, T., Sasaki, Y: "Homozygosity mapping of the locus responsible for renal tubular dysplasia of cattle on bovine chromosome 1."Mamm.Genome. 11. 316-319 (2000)
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「研究成果報告書概要(欧文)」より
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[Publications] Ohba, Y., Kitagawa, H., Kitoh, K., Sasaki, Y., Takami, M., Shinkai, Y., and Kunieda, T.: "A deletion of the paracellin-1 gene is responsible for renal tubular dysplasia in cattle."Genomics. 68. 229-236 (2000)
Description
「研究成果報告書概要(欧文)」より
