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2001 Fiscal Year Final Research Report Summary

Cloning of transporters for anionic drugs and the application to pharmacokinetic analysis

Research Project

Project/Area Number 11557005
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field General pharmacology
Research InstitutionKyorin University

Principal Investigator

KANAI Yoshikatsu  Kyorin University, School of Medicine, Professor, 医学部, 教授 (60204533)

Co-Investigator(Kenkyū-buntansha) HOSOYAMADA Makoto  Kyorin University, School of Medicine, Assistant Professor, 医学部, 講師 (00291659)
TAKEDA Michio  Kyorin University, School of Medicine, Associate Professor, 医学部, 助教授 (40255401)
ENDOU Hitoshi  Kyorin University, School of Medicine, Professor, 医学部, 教授 (20101115)
KIM Do Kyung  Kyorin University, School of Medicine, Research Associate, 医学部, 助手 (40327474)
Project Period (FY) 1999 – 2001
Keywordstransporter / organic anion / organic cation / drug transport / pharmacokinetics / blood-brain barrier / kidney / molecular cloning
Research Abstract

The purpose of this study is to identify new members of organic anion transporters of SLC22 family essential for the understanding of the pharmacokinetics of various drugs and xenobiotics. The final goal of this research is to establish the role of organic anion transporters in the pharmacokinetics by revealing the functions and tissue and cellular distribution of the human transporters. In the present study, we have identified six new members of the SLC22 family including OAT4 (organic anion transporter 4), OAT5, OAT6, OAT7, CT2 (carnitine transporter 2) and URAT1 (urate transporter 1). OAT4, OAT5 and OAT7 exhibit similar substrate selectivity preferring sulfate conjugates such as estrone sulgfate. OAT4 and OAT5 are present in the apical membrane of renal proximal tubules of human and rat, respectively. In contrast, OAT7 is present in the sinusoidal membrane of hepatocytes. Therefore, it is proposed that the sulfate conjugates formed in hepatocytes move into blood through OAT7 and taken up by renal proximal tubule epithelial cells via OAT1 and OAT3 on the basolateral membrane and excreted into urine via apical membrane OAT4 or OAT5. URAT1 is present in the apical membrane of renal proximal tubules and functions as an organic anion exchanger transporting urate, lactate, pyradine carboxylate and nicotinate. URAT 1 is responsible for the renal reabsorption of urate. Its genetic defect has turned out to be a cause of idiopathic renal hypouricemia. CT2 and OAT6 are carnitine transporters with different properties. It is notable that CT2 is expressed specifically in testis and is suggested to be important for sperm maturation. In addition, by generating cell lines stably expressing human organic anion transporters and analyzing the properties of the transport of various organic compounds using the cell lines, we have demonstrated that those cells lines are quite useful in the in vitro prediction of drug-drug interactions.

  • Research Products

    (104 results)

All Other

All Publications (104 results)

  • [Publications] Fukasawa, Y: "Identification and characterization of a Na+-independent neutral amino acid transporter that associates with the 4F2 heavy chain and exhibits substrate selectivity for small neutral D-and L-amino acids"J.Boil.Chem.. 275. 9690-9698 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakajima, N: "Developmental changes in multispecific organic anion transporter 1 expression in the rat kidney"Kidney Int.. 57. 1608-1616 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kanai, Y: "Transport properties of a system y+L neutral and basic amino acid transporter : Insights into the mechanisms of substrate recognition"J.Boil.Chem.. 275. 20787-20793 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakauchi, J.: "Cloning and characterization of a human brain Na+-independent transporter for small neutral amino acids that transports D-serine with high affinity"Neurosci.Lett.. 287. 231-238 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishii, H: "Involvement of rBAT in Na+ -dependent and -independent transport of the neurotransmitter candidate L-DOPA in Xenopus laevis oocytes injected with rabbit small intestinal epithelium poly A+RNA"Biochim.Biophys.Acta. 1466. 61-70 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsuo, H: "Mouse Asc-1 (asc-type amino acid transporter 1) maps to chromosome 7, region B1-B5"Chromosome Res.. 8. 456 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 金井好克: "アミノ酸トランスポーター:基質認識の多様性を生みだす分子基盤"シリーズ・ニューバイオフィジックス II:第3巻「ポンプとトランスポーター」、平田肇、茂木立志編(共立出版). 160-178 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Wada, S: "Rat multispecific organic anion transporter 1 (OAT1) transports zidovudine, acyclovir and antiviral nucleoside analogs"J.Pharmacol.Exp.Ther.. 294. 844-849 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kanai, Y: "Amino acid transporters : molecular structure and physiological roles"Nephrology, Dialysis, Transplantation. 15[Suppl 6]. 9-10 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsuo, H: "Expression of a system L neutral amino acid transporter at the blood-brain barrier"Neuroreport. 11. 3507-3511 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 金井好克: "グルタミン酸トランスポーターの機能特性"Brain Medical. 12. 161-168 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kageyama, T: "The 4F2hc/LAT 1 complex transporters L-DOPA across the blood-brain barrier"Brain Res.. 879. 115-121 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 金井好克: "ヘテロ二量体型アミノ酸トランスポーターファミリーの基質認識の機序"日本応用酵素協会誌. 36. 9-18 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tsuganezawa, H: "A new member of the HCO3-transporter superfamily is an apical anion exchanger of β-Intercalated Cells in the kidney"J.Biol.Chem.. 276. 8180-8189 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mizoguchi, K: "Human cystinuria-related transporter : localization and functional characterization"Kidney Int.. 59. 1821-1833 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kim, DK: "Expression cloning of a Na+-independent aromatic amino acid transporter with structural similarity to H+/monocarboxylate transporters"J.Biol.Chem.. 276. 17221-17228 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Cha, SH: "Identification and characterization of human organic anion transporter 3 expressing predominantly in the kidney"Mol.Pharmacol.. 59. 1277-1286 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kim, JY: "Human cystine/glutamate transporter : cDNA cloning and upregulation by oxidative stress in glioma cells"Biochim.Biophys.Acta. 1512. 335-344 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yanagida, O: "Human L-type amino acid transporter 1 (LAT1) : characterization of function and expression in tumor cell lines"Biochim.Biophys.Acta. 1514. 291-302 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 金井好克: "アミノ酸トランスポーター"Annual Review 腎臓 2001、伊藤克己, 浅野泰, 遠藤仁, 御手洗哲也, 東原英二編(中外医学社). 8 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nii, T: "Molecular events involved in up-regulating human Na+-independent neutral amino acid transporter LAT1 during T-cell activation"Biochem.J.. 358. 693-704 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Chairoungdua, A: "Identification and characterization of a novel member of the heterodimeric amino acid transporter family presumed to be associated with an unknown heavy chain"J.Biol.Chem.. 276. 49390-49399 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tamai, S: "Expression of L-type amino acid transporter 1 in a rat model of liver metastasis : positive correlation with tumor size"Cancer Detect.Prev.. 25. 439-445 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suzuki, K: "Transient upregulation of the glial glutamate transporter GLAST in response to fibroblast growth factor, insulin-like growth factor and epidermal growth factor in cultured astrocytes"J.Cell Sci.. 114. 3717-3725 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kim, DK: "The human T-type amino acid transporter 1 : characterization, gene organization and chromosomal location"Genomics. 79. 95-103 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Uchino, H: "Transport of amino acid-related compounds mediated by L-type amino acid transporter 1 (LAT1) : In sights into the mechanisms of substrate recognition"Mol.Pharmacol.. 61. 729-737 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kanai, Y: "The high-affinity glutamate neutral amino-acid transporter family : structure, function, and physiological relevance"Neurotransmitter transporters, M.E.A.Reith, Ed.(Humana Press, Totowa, N.J.). 57 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsuo, H: "Identification of a novel Na+-independent acidic amino acid transporter with structural similarity to the member of heterodimeric amino acid transporter family associated with unknown heavy chains"J.Biol.Chem.. 277. 21017-21026 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 金井好克: "多選択性アミノ酸トランスポーターファミリー:LATファミリー"蛋白質核酸酵素. 46. 629-637 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 金井好克: "小腸および腎におけるアミノ酸吸収を担うトランスポーター"医学のあゆみ. 199. 207-211 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kanai, Y: "Heterodimeric amino acid transporters : molecular biology and pathological and pharmacological relevance"Curr.Drug Metab.. 2. 339-354 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Enomoto, A: "Molecular identification of a renal urate-anion exchanger that regulates blood urate levels"Nature. 417. 447-452 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hosoya, K: "Enhancement of L-cystine transport activity and its relation to xCT gene induction at the blood-brain barrier by diethyl maleate treatment"J.Pharmacol.Exp.Ther.. 302. 225-231 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 金井好克: "中性アミノ酸トランスポーター-その分子実体と中枢神経における役割"シリーズ・バイオサイエンスの新世紀:第12巻「感覚器官と脳内情報処理」、御子柴克彦, 清水孝雄編(共立出版). 8 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Enomoto, A: "Molecular identification of a novel carnitine transporter specific to human testis : Insights into the mechanism of carnitine recognition"J.Biol.Chem.. 277. 36262-36271 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kim, DK: "Characterization of the system L amino acid transporter in T24 human bladder carcinoma cells"Biochim.Biophys.Acta. 1565. 112-122 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 金井好克: "多選択性中性アミノ酸トランスポーターLAT1の中枢神経における役割"脳の科学. 24. 367-377 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takeda, M: "Hydrogen peroxide downregulates human organic anion transporters in the basolateral membrane of the proximal tubule"Life Sci.. 68. 679-687 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sekine, T: "The multispecific organic anion transporter (OAT) family"Pflugers Arch.. 440. 337-350 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takeda, M: "Regulation by protein kinase C of organic anion transport driven by rat organic anion transporter 3 (rOAT3)"Life Sci.. 67. 1087-1093 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tsuda, M: "Transport of ochratoxin A by renal multispecific organic anion transporter 1"J.Pharmacol.Exp.Ther.. 289. 1301-1305 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hosoyamada, M.: "Molecular cloning and functional expression of a multispecific organic anion transporter from human kidney"Am.J.Physiol.. 276. F122-F128 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Jariyawat, S.: "The interaction and transport of b-lactam antibiotics with the cloned rat organic anion organic anion transporter 1"J.Pharmacol.Exp.Ther.. 290. 672-677 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Apiwattanakul, N.: "Transport properities of nonsteroidal anti-inflammatory drugs by organic anion transporter 1 expressed in Xenopus laevis oocytes"Mol.Pharmacol.. 55. 847-854 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Piyachaturawat, P.: "Effect of chronic K+ deficiency on contractile properties of soleus muscle in rats : Evidence of sex differences"Clin.Exp.Pharmacol.Physiol.. 26. 323-329 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hara, C.: "Intracellular pH regulatory mechanism in a human renal proximal cell line (HKC-8), evidence for Na+/H+ exchanger, Cl-/HCO3-excvhanger and Na+-HCO3-cotranspoter"Pluegers Arch. 440. 713-720 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Morita N.: "Functional characterization of rat organic anion transporter 2 in LLC-PK1 Cells"J.Pharmacol.Exp.Ther.. 298. 1179-1184 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sugiyama, D: "Characterization of the efflux transport of 17beta-estradiol-D-17beta-glucuronide from the brain across the blood-brain barrier"J Pharmacol Exp Ther.. 298. 316-232 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Usui, T: "Molecular basis of ocular abnormalities associated with proximal renal tubular acidosis"J.Clin Invest.. 108. 107-115 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takeda, M.: "Characterization of organic anion transport inhibitors using cells stably expressing human organic anion transporters"Eur.J.Pharmacol.. 419. 113-120 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Igarashi, T.: "Novel nonsense mutation in the Na+/HCO3-cotransporter gene (SLC4A4) in a patient with permanent isolated proximal renal tubular acidosis and bilateral glaucoma"J.Am.Soc Nephrol.. 12. 713-718 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Khamdang, S: "Interactions of human organic anion transporters and human organic cation transporters with nonsteroidal anti-inflammatory drugs"J Pharmacol Exp Ther.. 303. 534-549 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 金井好克: "アミノ酸輸送体:アミノ酸尿症"BIO Clinica. 14. 879-883 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 金井好克: "Na+ 依存性アミノ酸トランスポーター"生体の科学. 50. 291-297 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sekine, T: "Molecular biology of multispecific organic anion transporter family (OAT family)"Clin.Exp.Nephrol.. 3. 237-243 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 金井好克: "連載・臨床に必要な神経薬理「脳のトランスポーターと薬理(1)」グルタミン酸トランスポーター"Clinical Neuroscience. 18. 124-125 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 金井好克: "連載・臨床に必要な神経薬理「脳のトランスポーターと薬理(2)」モノアミントランスポーター"Clinical Neuroscience. 18. 246-247 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 金井好克: "連載・臨床に必要な神経薬理「脳のトランスポーターと薬理(3)」血液・脳関門トランスポーター"Clinical Neuroscience. 18. 368-369 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kanai, Y: "The family of amino acid transporters associated with type II membrane glycoproteins : the roles in epithelial transport and cellular nutrition. Control and diseses of sodium dependent transport proteins and ion channels"Elsevier Science B.V.. 311-314 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tsuda, M., Sekine, T., Takeda, M., Kanai, Y., Kimura, M. and Endou, H.: "Transport of ochratoxin A by renal multispecific organic anion transporter 1"J. Pharmacol. Exp. Ther.. 289. 1301-1305 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hosoyamada, M., Sekine, T., Kanai, Y. and Endou, H.: "Molecular cloning and functional expression of a multispecific organic anion transporter from humin kidney"Am. J. Physiol.. 276. F122-F128 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Jariyawat, S., Sekine, T., Apiwattanakul, N., Tsuda, M., Nakajima, N., Kanai, Y., Sophasan, S. and Endou, H.: "The interaction and transport of b-lactam antibiotics with the cloned rat organic anion organic anion transporter 1"J. Pharmacol. Exp. Ther.. 290. 672-677 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Apiwattanakul, N., Sekine, T., Chairoungdua, A., Kanai, Y., Nakajima, N., Sophasan, S. and Endou, H.: "Transport properties of nonsteroidal anti-inflammatoly drugs by organic anion transporter 1 expressed in Xenopus laevis oocytes"Mol. Pharmacol.. 55. 847-854 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Piyachaturawat, P., Muchimapura, S., Sophasan, S., Jariyawat, S., Pholpramool, C., Satayavivad, J. and Endou, H.: "Effect of chronic K+deficiency on contractile properties of soleus muscle in rats : Evidence of sex differences"Clin. Exp. Pharmacol. Physiol.. 26. 323-329 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsuo, H., Chairougdua, A., Kim, D.K., Cha, S.H., Fukuda, J., Endou, H. and Kanai, Y.: "Mouse Asc-1 (asc-type amino acid transporter 1) mapsa to chromosome 7, region B1-B5"Chromosome Res.. 8. 456 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hara, C., Satoh, H., Usui, T., Kunimi, M., Noiri, E., Tsukamoto, K., Taniguchi, S,, Uwatoko, S., Goto, A., Racusen., L.C., Inatomi, J., Endou, H., Fujita, T. and Seki, G.: "Intracellular pH regulatory mechanism in a human renal proximal cell line (HKC-8) : evidence for Na^+/H^+ exchanger, C1^-/HCO3^- exchanger and Na^+-HC03^- cotranspoter"Pluegers Arch. 440. 713-720 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsuo, H., Tsukada, S., Nakata, T., Chairoungdua, A., Kim, D.K, Cha, S.H., Inatomi, J., Yorifuji, H., Fukuda, J., Endou, H. and Kanai, Y.: "Expression of a system L neutral amino acid transporter at the blood-brain barrier"Neuroreport. 11. 3507-3511 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kanai, Y., Segwa, H., Chairoungdua, A., Kim, J.Y., Kim, D.K., Matsuo, H., Cha, S.H. and Endou, H.: "Amino acid transporters : molecular structure and physiological roles"Nephrol. Dial. Transplant. 15. 9-10 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takeda, M., Hosoyamada, M., Cha, SH., Sekine, T. and Endou, H.: "Hydrogen peroxide downregulates human organic anion transporters in the basolateral membrane of the proximal tubule"Life Sci.. 68. 679-687 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Wada, W., Tsuda, M., Sekine, T., Cha, S.H., Kimura, M., Kanai, Y. and Endou, H.: "Rat maltispecific organic anion transporter 1 (rOAT1) transports zidovudne, acyclovir, and other antiviral nucleoside analogs"J. Pharmacol. Exp, Ther.. 294. 844-849 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sekine, T., Cha, S.H., Endou, H.: "The multispecific organic anion transporter (OAT) family"Pflugers Arch.. 440. 337-350 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakauchi, J., Matsuo, H., Kim, DK., Goto, A., Chairoungdua, A., Cha, SH., Inatomi, J., Shiokawa, Y., Yamaguchi, K., Saito, I., Endou, H. and Kanai, Y.: "Cloning and characterization of a human brain Na^+-independent transporter for small neutral amino acids that transports D-serine with high affinity"Neurosci. Lett.. 287. 231-238 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takeda, M., Sekine, T. and Endou, H.: "Regulation by protein kinase C of organic anion transport driven by rat organic anion transporter 3 (rOAT3)"Life Sci.. 67. 1087-1093 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakajima, N., Sekine, T., Cha, S.H., Tojo, A., Hosoyamada, M., Kanai, Y., Yan, K., Awa, S. and Endou, H.: "Developmental changes in multispecific organic anion transporter 1 (OAT1) expression in the rat kidney"Kidney Int.. 57. 1608-1618 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kanai, Y., Fukasawa, Y., Cha, S.H., Segawa, H., Chairoungdua, A., Kim, DK., Matsuo, H., Kim, JY, Miyamoto, K., Takeda, E. and Endou, H.: "Transport properties of a system y+L neutral and basic amino acid transporter"J. Biol. Chem.. 275. 20787-20793 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fukasawa, Y., Segawa, H., Kim, J.Y., Chairoungdua, A., Kim, DK., Endou, H. and Kanai, Y.: "Identification and characterization of a Na+-independent neutral amino acid transporter which associates with the 4F2 heavy chain and exhibits selectivity for small neutral D- and L-amino acids"J. Biol. Chem.. 275. 9690-9698 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okuda, T., Haga, T., Kanai, Y., Endou, H., Ishihara, T. and Katsura, I.: "Identification and characterization of the high-affinity choline transporter"Nature Neurosci.. 3. 120-125 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Cha, S.H., Sekine, T., Kusuhara, H., Yu, E., Kim, J.Y., Kim, D.Y., Sugiyama, Y., Kanai, Y. and Endou, H.: "Molecular cloning and characterization of multispecific organic anion transporter 4 expressed in the placenta"J. Biol. Chem.. 275. 4507-4512 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Chairoungdua, A., Kanai, Y., Matsuo, H., Inatomi, J., Kim, DK., and Endou, H.: "Identification and characterization of a novel member of the heterodimeric amino acid transporter family presumed to be associated with an unknown heavy chain"J. Biol. Chem.. 276. 49390-49399 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Morita, N., Kusuhara, H., Sekine, T., Endou, H. and Sugiyama, Y.: "Functional characterization of rat organic anion transporter 2 in LLC-PK1 Cells"J. Pharmacol. Exp. Ther.. 298. 1179-1184 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yanagida, 0., Kanai, Y., Chairoungdua, A., Kim, DK., Segawa, H., Nii, T., Cha, SH., Matsuo, H., Fukushima, J., Fukasawa, Y., Tani, Y., Taketani, Y., Uchino, H., Kim, JY., Inatomi, J., Okayasu, I., Miyamoto, K., Takeda, E., Goya, T., Endou, H.: "Human L-type amino acid transporter 1 (LAT1) : characterization of function and expression in tumor cell lines"Biochim Biophys Acta. 1514(2). 291-302 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nii, T., Segawa, H., Taketani, Y., Tani, Y., Ohkido, M., Kishida, S., Ito, M., Endou, H., Kanai, Y., Takeda, E. and Miyamoto, K.: "Molecular events involved in up-regulating huma Na+-indenendent neutral amino acid transporter LAT1 during T-cell activation"Biochem. J.. 358. 693-704 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sugiyama, D., Kusuhara, H., Shitara, Y., Abe, T., Meier, PJ., Sekine, T., Endou, H., Suzuki, H., Sugiyama, Y.: "Characterization of the efflux transport of 17beta-estradiol-D-17beta-glucuronide from the brain across the blood-brain barrier"J. Pharmacol. Exp. Ther.. 298. 316-322 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Usui, T., Hara, M., Satoh, H., Moriyama, N., Kagaya, H., Amano, S., Oshika, T., Ishii, Y., Ibaraki, N., Hara, C., Kunimi, M., Noiri, E., Tsukamoto, K., Inatomi, J., Kawakami, H., Endou, H., Igarashi, T., Goto, A., Fujita, T., Araie, M., Seki, G.: "Molecular basis of ocular abnormalities associated with proximal renal tubular acidosis"J. Clin. Invest.. 108(1). 107-115 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kim, J.Y., Kanai, Y., Chairoungdua, A., Cha, S.H., Matsuo, H., Kim, D.K., Inatomi, J., Sawa, H., Ida, Y. and Endou, H.: "Human cystine/glutamate transporter : cDNA cloning and upregulation by oxidative stress in glioma cells"Biochem. Biopys. Acta. 1512. 335-344 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takeda, M., Narikawa, S., Hosoyamada, M., Cha, S.H., Sekine, T. and Endou, H.: "Characterizaion of organic anion transport inhibitors using cells stably expressing human organic anion tansporters"Eur. J. Pharmacol.. 419. 113-120 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kim, DK., Kanai, Y., Chairoungdua, A., Matsuo, H., Cha, SH. and Endou, H.: "Expression cloning of a Na^+-independent aromatic amino acid transporter with structural similarity to H^+/monocarboxylate transporters"J. Biol. Chem.. 276(20). 17221-17228 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mizoguchi, K., Cha, SH., Chairoungdua, A., Kim, DK., Shigeta, Y., Matsuo, H., Fukushima, J., Awa, Y., Akakura, K,, Goya, T., Ito, H., Endou, H. and Kanai, Y.: "Human cystinuria-related transporter : Localization and functional characterization"Kidney Int.. 59. 1821-1833 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Cha, S.H., Sekine, T., Fukushima, J., Kanai, Y., KobayasHi, Y., Goya, T. and Endou, H.: "Identification and characterization of human organic anion transporter 3 expressing predominanatly in the kidney"Mol. Pharmacol.. 59. 1277-1286 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Igarashi, T., Inatomi, J., Sekine, T., Seki, G., Shimadzu, M., Tozawa, F., Takeshima, Y., Takumi, T., Takahashi, T., Yoshikawa, N., Nakamura, H. and Endou, H.: "Novel nonsense mutation in the Na^+/HCO_3 cotransporter gene (SLC4A4) in a patient with permanent isolated proximal renal tubular acidosis and bilateral glaucoma"J. Am. Soc. Nephrol.. 12. 713-718 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsuganezawa, H., Kobayashi, K., Iyori, M., Araki, T., Koizumi, A., Watanabe, S., Kaneko, A., Fukao, T., Monkawa, T., Yoshida, T., Kim, DK., Kanai, Y., Endou, H., Hayashi, M. and Saruta, T.: "A new member of the HC03- transporter superfamily is an apical anion exchanger of b-Intercalated Cells in the kidney"J. Biol. Chem.. 276. 8180-8189 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kim, DK., Kanai, Y., Choi, HW., Tangtrongsup, S., Chairoungdua, A., Babu, E., Tachampa, K., Anzai, N., Iribe, Y. and Endou, H.: "Characterization of the system L amino acid transporter in T24 human bladder carcinoma cells"Biochim. Biophys. Acta. 1565. 112-122 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Enomoto, A., Wempe, MF., Tsuchida, H., Shin, HJ., Cha, SH., Anzai, N., Goto, A., Sakamoto, A., Niwa, T., Kanai Y., Anders, MW., Endou H.: "Molecular identification of a novel carnitine transporter specific to humantestis : Insights into the mechanism of carnitine recognition"J. Biol. Chem.. 277. 36262-36271 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hosoya, K., Tomi, M., Ohtsuki, S., Takanaga, H., Saeki, S., Kanai, Y., Endou, H., Naito, M., Tsuruo, T., Terasaki, T.: "Enhancement of L-cystine transport activity and its relation to xCT gene induction at the blood-brain barrier by diethyl maleate treatment"J. Pharmacol. Exp. Ther.. 302. 225-231 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Enomoto, A., Kimura, H., Shigeta, Y., Ikeda, M., Cha, SH., Hosoyamada, M., Takeda, M., Sekine, T., Igarashi, T., Matsuo, H., Kikuchi, Y., Oda, T., Ichida, K., Hosoya, T., Shimokata, K., Niwa, T., Kanai, Y. and Endou, H.: "Molecular identification of a renal urate-anion exchanger that regulates blood urate levels"Nature. 417. 447-452 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Khamdang, S., Takeda, M., Noshiro, R., Narikawa, S., Enomoto, A., Anzai, N., Piyachaturawat, P., Endou, H.: "Interactions of human organic anion transporters and human organic cation transporters with nonsteroidal anti-inflammatory drugs. J Pharmacol Exp Ther. 303 : 534-9, (2002) anti-inflammatory drugs"J. Phamacol. Exp. Ther.. 303(2). 534-539 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsuo, H., Kanai, Y., Kim, JY., Chairoungdua, A., Kim, D.K., Inatomi, J., Shigeta, Y., Ishimine, H., Chaekuntode, S., Tachampa, K., Choi, HW., Babu, E., Fukuda,J. and Endou, H.: "Identification of a novel Na^+-independent acidic amino acid transporter with structural similarity to the member of heterodimeric amino acid transporter family associated with unknown heavy chains"J. Biol. Chem.. 277. 21017-21026 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Uchino, H., Kanai, Y., Kim, DK., Wempe, MF,, Chairoungdua, A., Morimoto, E., Anders, MW. and Endou, H.: "Transport of amino acid-related compounds mediated by L-type amino acid transporter 1 (LAT1) : Insights into the mechanisms of substrate recognition"Mol. Pharmacol.. 61. 729-737 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kim, DK., Kanai, Y., Matsuo, H., Kim, JY., Chairoungdua, A., Kobayashi, Y., Enomoto, A., Cha, SH., Goya, T. and Endou, H.: "The human T-type amino acid transporter 1 : characterization, gene organization and hromosomal location"Genomics. 79. 95-103 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sekine, T., Seok Ho Cha, Kanai, Y. and Endou, H.: "Molecular biology of multispecific organic anion transporter family (OAT family)"Clin. Exp. Nephrol.. 3. 237-243 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kanai, Y., and Endou, H.: "Heterodimeric amino acid transporters : molecular biology and pathological and pharmacological relevanc"Curr. Drug Metab. 2. 339-354 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takeda, M., Endou, H.: "chapter 9. Renal Cell Metabolism""Massry & Glassock's Textbook of Nephrology, Fourth Edition" E (Shaul G. Massry and Richard J. Glassock eds.). 110-121 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kanai, Y., Segawa, H., Chairoungdua, A., Kim, JY., Kim, DK. and Endoh, H.: "The family of amino acid transporters associated with type II membrane glycoproteins : the roles in epithelial transort and cellular nutrition""Control and diseses of sodium dependent transport proteins and ion channels", Y.Suketa, E.Carafoli, M.Lazdunski, K.Mikoshiba, Y.Okada, Eds., (Elsevier Science B.V.). 311-314 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kanai, Y., Trotti, D., Berger, UV. and Hediger, MA.: "The high-affinity glutamate neutral amino-acid transporter family : structure, function, and physiological relevance""Neurotransmitter transporters : Structure, function and regulations, 2nd ed.", M.E.A.Reith, Ed., (Humana Press, Totowa, N. J.). 255-311 (2002)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2003-09-17  

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