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2000 Fiscal Year Final Research Report Summary

Identification of novel cytoplasmic factors responsible for cell growth and death.

Research Project

Project/Area Number 11557007
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section展開研究
Research Field General medical chemistry
Research InstitutionKansai Medical University

Principal Investigator

KUROSAKI Tomohiro  Kansai Medical University, Department of Medicine, Professor, 医学部, 教授 (50178125)

Co-Investigator(Kenkyū-buntansha) OKAWA Katsuya  Kirin Brewery Co., LTD., Investigator, 研究員
IWAMATSU Akihiro  Kirin Brewery Co., LTD., Principal Investigator, 主任研究員
ISHIAI Masamichi  Kansai Medical University, Department of Medicine, Assistant Professor, 医学部, 助手 (90298844)
Project Period (FY) 1999 – 2000
KeywordsBCR signaling / Syk / BLNK / BCAP / apoptosis / 遺伝子欠損 / 微量蛋白質精製
Research Abstract

The ability of B cells to respond to antigen relies on signals transmitted through the B cell antigen receptor (BCR) complex. Activation of cytoplasmic protein tyrosine kinases (PTKs) is the earliest measurable biochemical response to BCR cross-linking. The initial event leads to the generation of secondary signals including Ras activation, phosphatidylinositol 3-kinase (PI-3K) activation, phospholipase C (PLC)-γ2 activation.
Based upon our previous evidence that Syk, a BCR-associated PTK, is essential for PLC-γ2 activation, we purified tyrosine-phosphorylated proteins mediated by Syk. Among 15 purified proteins, two molecules (BLNK and BCAP) were turned out to play an important role in BCR-mediated apoptosis. In fact, BLNK-deficient B cells abolished apoptosis, whereas this BCR-mediated apoptosis was enhanced by loss of BCAP.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Ishiai,M. et al.: "BLNK required for coupling Syk to PLCγ2 and Racl-JNK in B cells."Immunity. 10. 117-125 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kurosaki,T.: "Genetic analysis of B cell antigen receptor signaling."Annu.Rev.Immunol.. 17. 555-592 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishiai,M. et al.: "Association of Phospholipase C-γ2 Src homology 2 domains with BLNK is critical for B cell antigen receptor signaling."J.Immunol.. 163. 1746-1749 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hashimoto,A. et al.: "Essential role of phospholopase C-γ2 in B cell development and function."J.Immunol.. 165. 1738-1742 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishiai,M. et al.: "Involvement of LAT, Gads, and Grb2 in compartmentation of SLP-76 to the plasma membrane."J.Exp.Med.. 192. 847-856 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okada,T. et al.: "BCAP : The tyrosine kinase substrate that connects B cell receptor to phosphoinositide 3-kinase activation."Immunity. 13. 817-827 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishiai, M., et al.: "BLNK required for coupling Syk to PLCγ2 and Rac1-JNK in B cells."Immunity. 10. 117-125 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kurosaki, T.: "Genetic analysis of B cell antigen receptor signaling."Annu.Rev.Immunol.. 17. 555-592 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishiai, M., et al.: "Association of Phospholipase C-γ2 Src homology 2 domains with BLNK is critical for B cell antigen receptor signaling."J.Immunol.. 163. 1746-1749 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hashimoto, A., et al.: "Essential role of phospholopase C-γ2 in B cell development and function."J.Immunol.. 165. 1738-1742 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishiai, M., et al.: "Involvement of LAT, Gads, and Grb2 in compartmentation of SLP-76 to the plasma membrane."J.Exp.Med.. 192. 847-856 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okada, T., et al.: "BCAP : The tyrosine kinase substrate that connects B cell receptor to phosphoinositide 3-kinase activation."Immunity. 13. 817-827 (2000)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2002-03-26  

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