2001 Fiscal Year Final Research Report Summary
DEVELOPMENT OF CLONE TRANSGENIC RABBITS FOR THE STUDY OF ATHEROSCLEROSIS
| Project/Area Number |
11557016
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Human pathology
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| Research Institution | SAGA MEDICAL SCHOOL (2000-2001)
University of Tsukuba (1999) |
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Principal Investigator |
WATANABE Teruo SAGA MEDICAL SCHOOL, VICE PRESIDENT, 副学長 (40037396)
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| Co-Investigator(Kenkyū-buntansha) |
HONDA Kazuo YAMANOUCHI TSUKUBA RESEARCH INSTITUTE, PRESIDENT, 筑波研究所, 薬理研究所長
FAN Jianglin UNIVERSITY OF TSUKUBA, INSTITUTE OF BASIC MEDICAL SCIENCES, LECTURER, 基礎医学系, 講師 (60272192)
KIMOTO Masao SAGA MED SCHOOL, IMMUNOLOY, PROFESSOR, 医学部, 教授 (40153225)
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| Project Period (FY) |
1999 – 2001
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| Keywords | clone / animal cloning / hypercholesterolemia / atherosclerosis / animal models / transgenic rabbit / transgene / lipoproteins |
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| Research Abstract |
Transgenic rabbits are widely used as both bioreactors for the production of therapeutic proteins and human disease models in many biomedical fields. In attempt to generate transgenic rabbits by nuclear transfer technique, we performed the initial trial in making clone rabbits using somatic cells from transgenic rabbits and herewith we have optimized several factors that may be involved in the success of nuclear transfer procedures such as culture media, fusion and activation conditions and other related factors. Both electric fusion and piezo methods were used in this study. Although the generation of clone rabbits using somatic cells is still in immature state, it seems that compared to other species (goat, sheep, cow, mouse and pig), rabbit cloning using somatic cell nuclear transfer is rather complicated and a lot of procedures need to be refined before we can succeed in making cloned rabbits. In the second part of the study, we investigated transgenic rabbits expressing human apo(a) in terms of Lp(a) atherogenicity after cholesterol diet feeding. We found that transgenic rabbits developed greater atherosclerosis in aorta and coronary arteries than did control rabbits.
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Research Products
(19 results)
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[Publications] Ichikawa, T., Unoki, H., Sun, H., Shimoyamada, H., Shikama, T., Watanabe, T., Fan, J.: "Lipoprotein(a) promotes smooth muscle cell proliferation and dedifferentiation in atherosclerotic lesions of human apo(a) transgenic rabbits"Am J. Pathol.. 160. 227-236 (2002)
Description
「研究成果報告書概要(和文)」より
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[Publications] Fan, J., Unoki, H., Kojima, N., Sun, H., Shimoyamada, H., Watanabe, T.: "Overexpression of lipoprotein lipase in transgenic rabbits inhibits diet-induced hypercholesterolemia and atherosclerosis"J. Biol. Chem.. 276. 40071-40079 (2001)
Description
「研究成果報告書概要(和文)」より
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[Publications] Wu, L., Tanimoto, A., Murata, Y., Fan, J., Sasaguri, Y., Watanabe, T.: "Induction of human metalloproteinase-12 gene transcritional activity by GM-CSF requires the AP-1 binding site in human U937 monocytic cells"Biochem. Biophys. Res. Commun.. 285. 300-307 (2001)
Description
「研究成果報告書概要(和文)」より
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[Publications] Ichikawa, T. Unoki, H.,l Sun, H., Shimoyamada, H., Shikama, T., Watanabe, T., Fan, J.: "Lipoprotein(a) promotes smooth muscle cell proliferation and dedifferentiation in atherosclerotic lesions of human apo(a) transgenic rabbits"Am. J. Pathol.. 160. 227-236 (2002)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Fan, J., Unoki, H., Kojima, N., Sun, H., Shimoyamada, H., Deng, H., Yamada, N., Watanabe, T.: "Overexpression of lipoprotein lipase in transgenic rabbits inhibits diet-induced hypercholesterolemia and atherosclerosis"J. Biol. Chem.. 276. 40071-40079 (2001)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Wu, L., Tanimoto, A., Murata, Y., fan, J., Sasaguri, Y., Watanabe, T: "Induction of human metalloproteinase-12 gene transcritional activity by GM-CSF requires the AP-1 binding site in human U937 monocytic cells"Bikochem. Biophys. Res. Commun.. 285. 300-307 (2001)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Fan, J., Shimoyamada, H., Sun, H., Marcovina, S., Honda, K., Watanabe, T.: "Transgenic rabbits expressing hunman apolipoprotein(a) develop enhanced altherosclerotic lesions in response to a cholesterol-rich diet"Arterioscl. Thromb. Vascul. Biol.. 21. 88-94 (2001)
Description
「研究成果報告書概要(欧文)」より
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