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2002 Fiscal Year Final Research Report Summary

Study on human T cell changes in response to infections and applications

Research Project

Project/Area Number 11557023
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Bacteriology (including Mycology)
Research InstitutionTokyo Women's Medical University School of Medicine

Principal Investigator

IMANISHI Ken-ichi  Tokyo Women's Medical University School of Medicine, Microbiology and Immunology, Associate Professor, 医学部, 助教授 (20132920)

Co-Investigator(Kenkyū-buntansha) FUJIMAKI Wakae  Tokyo Women's Medical University School of Medicine, a medical school, Professor, 医学部, 助手 (90256496)
YAGI Junji  Tokyo Women's Medical University School of Medicine, a medical school, Professor, 医学部, 講師 (70182300)
UCHIYAMA Takehiko  Tokyo Women's Medical University School of Medicine, a medical school, Professor, 医学部, 教授 (00050550)
AKIYAMA TOHRU (MIYOSHI Tohru)  Tokyo Women's Medical University School of Medicine, a medical school, Professor, 医学部, 助手 (20246466)
KATO Hidehito  Tokyo Women's Medical University School of Medicine, a medical school, Professor, 医学部, 助手 (00241084)
Project Period (FY) 1999 – 2002
KeywordsT cells / superantigen / infectious diseases / γδT cells / toxic shock syndrome / renal cell carcinoma / neonatal toxic shock syndrome-like exanthematous disease
Research Abstract

1. By analyzing T cell receptor (TCR) Vβ2 vs CD45RO expression in T cells from patients with neonatal exanthematous disease in acute phase, we discovered an emerging neonatal infectious disease, neonatal toxic shock syndrome-like exanthematous disease (NTED), which is induced by TSST-1 produced by methicillin-resistant Staphylococcus aureus (MRSA). Using the same method, we provided early and definitive diagnosis of toxic shock syndrome (TSS). Furthermore, we observed that, in the early acute phase of NTED patients, there was a significant decrease in the percentage of Vβ2^+ T cells in peripheral blood T cells before the usual increase.
2. To see abnormal reactions seen in TSS we made two models using rabbits and mice by long-term exposure to superantigen in implanted osmotic pumps. In the model using rabbits, we observed acute and systemic abnormal reactions and very high mortality rate. In the model using mice, we found that the level of the superantigen-reactive CD4^+ T cell fraction … More s varies widely depending on TCR Vβ elements expressed and that the reactive CD4^+ T cells acquire a capacity to raise a memory response. CD8^+ T cells are low responders to superantigen.
3. The study of T cell changes in response to infection is useful not only for humans but also other animals. Streptococcus dysgalactiae-derived mitogen (SDM) was recently identified from a bovine isolate of S. dysgalactiae. A comparison of its effects on human and bovine PBMC revealed that it acts as a superantigen on both species. The superantigen SpeC, which is a product of the predominantly human pathogen Streptococcus pyogenes, also activates corresponding T cells in human and bovine systems. SDM however, has a much more potent effect on bovine cells than human cells and is more likely to have a pathogenic role in S. dysgalactiae infections of cows than humans.
4. It is known that human γδT cells defend the body against infection. We observed that the number of γδT cells was elevated in the peripheral blood in patients with renal cell carcinomas (RCC), and that the level of the γδT cells decreased after surgery. Our finding suggest that γδT cells provide innate immunity against RCC. Less

  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Mwaka Monze: "Superantigenic stimulation of bovine T cells by Streptococcus dysgalactiae-derived mitogen (SDM)"東京女子医科大学雑誌. (印刷中). (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 松田義雄: "Early and definitive diagnosis of toxic shock syndrome by detection of marked expansion of T cell receptor Vβ2-positive T cells"Emerging Infectious Diseases. 9. 387-389 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 三好・秋山徹: "A novel vacterial superantigen, Strptococcus dysgalactiae-derived mitogen (sdm) -characterization of its blological activity and predicted tertiary structure"Molecular Microbiology. (印刷中). (2003)

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      「研究成果報告書概要(和文)」より
  • [Publications] 陳露秋: "Continuous exposure of mice to superantigenic toxins induces a high-level protracted expansion and an immunological memory in the toxin-reactive CD4^+ T cells"Journal of Immunology. 168. 3817-3824 (2002)

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      「研究成果報告書概要(和文)」より
  • [Publications] 加藤秀人: "The percentage of superantigen-reactive T cells in peripheral blood signiflcantly decreases before massively increasing in patients with neonatal TSS-like exanthematous disease in the early acute phase"Journal of Infection and Chemotherapy. 8. 111-114 (2002)

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      「研究成果報告書概要(和文)」より
  • [Publications] 内山竹彦: "新生児のMRSA感染症;新生児TSS様発疹症(NTED)とスーパー抗原性毒素"感染・炎症・免疫. 31. 198-206 (2001)

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      「研究成果報告書概要(和文)」より
  • [Publications] 小林博人: "Gamma/delta T cells provide innate immunity against renal cell carcinoma"Cancer immunology and Immunotherapy. 50. 115-124 (2001)

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      「研究成果報告書概要(和文)」より
  • [Publications] 藤巻わかえ: "Functional uncoupling of TCR engagement and Lck activation in anergic human thymic CD4^+ T cells"Journal of Biological Chemstry. 276. 17455-17460 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 高橋尚人: "Immunopathophysiological aspects of an emerging neonatal infectious disease induced by a bacterial superantigen"Journal of Clinical Investigation. 106. 1409-1415 (2000)

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      「研究成果報告書概要(和文)」より
  • [Publications] 今西健一: "ヒトT細胞の胸腺内および胸腺後の発達(CD4^+シングルポジティブT細胞について)"アレルギー・免疫. 6. 108-117 (1999)

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      「研究成果報告書概要(和文)」より
  • [Publications] 内山竹彦: "The pathogenesis of Kawasaki disease and superantigens"Japan Journal of infectious Diseases. 52. 141-145 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 河内章: "ブドウ球菌スーパー抗原TSST-1の長時間暴露によるウサギ毒素性ショック症候群の誘導とその病態の解析"東京女子医科大学雑誌. 69. 571-578 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M. Monze: "Superantigenic stimulation of bovine T cells by Streptococcus dysgalactiae-derived mitogen (SDM)."J. Tokyo Wom. Med. Univ.. (in press). (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y. Matsuda: "Early and definitive diagnosis of toxic shock syndrome by detection of marked expansion of T cell receptor Vβ2-positive T cells."Emerg. Infect. Dis.. 9. 387-389 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Miyoshi-Akiyama: "A novel bacterial superantigen, Streptococcus dysgalactiae-derived mitogen (sdm) - characterization of its biological activity and predicted tertiary structure."Mol. Microbiol.. (in press). (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] L. Chen: "Continuous exposure of mice to superantigenic toxins induces a high-level protracted expansion and an immunological memory in the toxin-reactive CD4^+ T cells."J. Immunol.. 168. 3817-3824 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Kato: "The percentage of superantigen-reactive T cells in peripheral blood significantly decreases before massively increasing in patients with neonatal TSS-like exanthematous disease in the early acute phase."J. Infect. Chemother.. 8. 111-114 (2002)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Y. Arimura: "A co-stimulatory molecule on activated T cells, H4/ICOS, delivers specific signals in Th cells and regulates their responses."Int. Immunol.. 14. 555-566 (2002)

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      「研究成果報告書概要(欧文)」より
  • [Publications] H. Kobayashi: "Gamma/delta T cells provide innate immunity against renal cell carcinoma."Cancer Immunol. Immunother.. 50. 115-1124 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] W. Fujimaki: "Functional uncoupling of TCR engagement and Lck activation in anergic human thymic CD4^+ T cells."J. Biol. Chem.. 276. 17455-17460 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] N. Takahashi: "Immunopathophysiological aspects of an emerging neonatal infectious disease Induced by a bacterial superantigen."J. Clin. Invest.. 106. 1409-1415 (2000)

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      「研究成果報告書概要(欧文)」より
  • [Publications] K. Imanishi: "Intra- and post-thymic development of human T cells."Allergology & Immunology. 6. 108-117 (1999)

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      「研究成果報告書概要(欧文)」より
  • [Publications] J. Yagi: "Identification of a new type of invariant Vα14^+T cells and responsiveness to a superantigen, Yersinia pseudotuberculosis-derived mitogen."J. Immunol.. 163. 3083-3091 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Uchiyama: "The pathogenesis of Kawasaki disease and superantigens."Jpn J. infect. Dis.. 52. 141-145 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A. Kawachi: "Pathogenic mechanism of toxic shock syndrome in rabbits induced by long-term exposure to staphylococcal superantigen TSST-1."J. Tokyo Wom. Med. Univ.. 69. 571-578 (1999)

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Published: 2004-04-14   Modified: 2021-11-22  

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