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2000 Fiscal Year Final Research Report Summary

Development of a method for measurement of a time-weighted average concentration of polycyclic mutagens in marine environment-for practiced utilization in monitoring of marine environment.

Research Project

Project/Area Number 11557030
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section展開研究
Research Field Hygiene
Research InstitutionOKAYAMA UNIVERSITY

Principal Investigator

KIRA Shohei  Okayama University, Medical School, Department of Public Health, Professor, 医学部, 教授 (50033212)

Co-Investigator(Kenkyū-buntansha) ITO Takehiko  Okayama University, Faculty of Education, Associate professor, 教育学部, 助教授 (10291973)
HAYATSU Hikoya  Okayama University, Faculty of Pharmaceutical Science, Professor emeritus, 名誉教授 (10012593)
NOGAMI Yusaku  Okayama University of Science, Faculty of Science, Professor, 理学部, 教授 (00172768)
FUJISAWA Kuniyasu  Fisheries Experiment Station of Okayama Prefecture, Senior researcher, 業務部, 専門研究員
KOMATSU Teruhisa  University of Tokyo, Ocean Research Institute, Associate professor, 海洋研究所, 助教授 (60215390)
Project Period (FY) 1999 – 2000
Keywordsmutagenicity / polycyclic aromatic compounds / water environment / time-weighted average concentration / sampling device / blue rayon / water flow intensity
Research Abstract

(1) Results of measuring the time-weighted average (TWA) concentration of Benzo (a) pyrene (BaP) in the Seto Inland Sea, Japan.
The values of the TWA concentration of BaP from 1999 to 2000 ranged from 0.16 to 8.77 ng/L for the sampling sites in Okayama seashore, from 0.08 to 7.04 ng/L for Osaka Bay sites and from undetectable level (0.01 ng/L) to 18.88 ng/L for the sites in Northern Kyushu.
(2) Effects of water temperature on the decrease in weight of plaster balls.
We gave a dissolving test by hanging a plaster ball in moving water and found that the decrease in weight of the plaster ball correlated well with water flow intensity, and the regression coefficient and the intercept of regression line were affected by water temperature. We obtained the following equation :
C={ΔW-(0.1232T+0.2247)}/(0.0189T+0.8162)
where C is water flow intensity (cm/sec), ΔW is the decrease in weight of plaster ball (g) and T is water temperature (℃) and Therefore, according to this equation, it becomes possibl … More e to correct the value of measured TWA if water temperature is known.
(3) Preserving condition of blue rayon after hanging and the recovery rate of BaP.
One nanogram of BaP was dissolved in distilled water in a beaker and let BaP adsorbed to BR.We then calculated the recovery rate from amount of BaP after keeping it under various conditions against the initial one that was obtained from extraction without preservation. They were 101.6±8.2% (for one week) and 100.0±6.8% (for two weeks) when BR was preserved with aluminium-foil-wrapping at -5℃, and 93.5±5.1% (for four weeks) when preserved with transparent-plastic-foil-wrapping at 15-2O℃. The amount of the BaP adsorbed to BR has maintained over 90% even in the room temperature. Our study provides a convenient and a practical procedure for long-distance transportation of the samples.
(4) Relationship between the amount of BaP and mutagenicity in samples after BR hanging.
We have reported that mutagenicity detected by fhe BR hanging technique positively correlated with the amount of BaP.The present study unexpectedly found some samples despite of small amount of BaP showed high mutagenicity. Most of these phenomena were observed on the samples hanging in estuaries of the rivers flowing through the cities. This fact suggests that the city sewages might contain highly mutagenic substances except for BaP.
(5) Publications and presentation of the study.
We have published and presented the study results in scientific journals and at some conferences (see references). Furthermore, we are preparing for presentations at following international symposiums in order to receive various evaluations from foreign scientists : (1) PRIMO11 (July 2001, Plymouth, UK), (2) ISTA10 (August 2001, Quebec, Canada), (3) 8thICEM (October 2001, Shizuoka, Japan). Less

  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Kira S.: "Measurement of benzo (a) pyrene in sea water and in mussels in the Seto inland sea, Japan"Bull.Environ.Contam.Toxicol. 65(5). 631-637 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 吉良尚平: "海のベンツピレン汚染"月刊「水」. 42(15). 16-23 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nogami Y.: "Benzo (a) pyrene adsorbed to suspended solid in fresh water"Environ.Toxicol. 15(5). 500-503 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 早津彦哉: "ポリマー担体に固着した色素の反応:ブルーレーヨン、ブルーキチン、グリーンキトサンなどの作製とその応用"薬学雑誌. 120(6). 534-547 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kira S.: "Detection of benzo (a) pyrene and mutagenicity in water of Lake Bikal (Rossia) and rivers in Okayama (Japan) using the blue rayon method : a simplified handling and transportation of samples from remote sites."Environmental Toxicology and Water Quality. 14(2). 279-284 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 野上祐作: "活性汚泥による有機基質の初期吸着に関する考察"水処理技術. 40(1). 9-12 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 古島靖夫: "簡便な流動場計測の試み"平成12年度日本水産工学会学術講演会講演論文集. 217-220 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Furushima Y.: "Attempt to achieve simple measurement of water motion in shallow areas."OCEANS 2000 MTS/IEEE Proceedings. 2. 993-997 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Otsu R.: "Propeties and mutagenicity of air pollutant in the southern part of Korea."First Asia Aerosol Conference, July. 27-29 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 野上祐作: "水環境中の懸濁粒子に吸着されている多環芳香族炭化水素の測定"第35回水環境学会年会(岐阜)、3月. (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中山祥嗣: "改良型ブルーレーヨン(BR)懸垂法による海水中Benzo(a)pyreneの時間平均値(TWA)の測定"日本環境変異原学会第29回大会(仙台)要旨集、11月. (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kira S.: "Measurement of benzo (a) pyrene in sea water and in mussels in the Seto inland sea, Japan."Bull.Environ.Contam.Toxicol.. 65(2). 631-637 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kira S.: "Marine pollution and Benzo (a) pyrene."Gekkan Mizu. 42(15). 16-23 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nogami Y.: "Benzo (a) pyrene adsorbed to suspended solid in fresh water."Environ.Toxical.. 15. 500-503 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hayatsu H.: "Pigment Molecules Linked to Polymer Support : Blue Rayon, Blue Chitin, and Green Chitosan-Synthesis and Applications."Yakugaku Zasshi. 120(6). 534-547 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kira S.: "Detection of benzo (a) pyrene and mutagenicity in water of Lake Bikal (Russia) and rivers in Okayama (Japan) using the blue rayon method : a simplified handling and transportation of samples from remote sites."Environmental Toxicology and Water Quality. 14(2). 279-284 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nogami Y.: "Studies on the primary adsorption of organic substrates by activated sludge."Mizushorigijutsu. 40(1). 9-12 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Furushima Y.: "Attempt to achieve simple measurement of water motion."Proceedings of the Annual Meeting of the Japanese Society of Fisheries Engineering. 217-220 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Furushima Y.: "Attempt to achieve simple measurement of water motion in shallow areas."OCEANS 2000 MTS/IEEE Proceedings. 2. 993-997 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Otsu R.: "Properties and mutagenicity of air pollutant in the southern part of Korea."First Asia Aerosol Conference. 27-29 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nogami Y.: "Polycyclic Aromatic Hydrocarbons Adsorbed to Suspended Solid in Fresh Water."Abstract for 35th Annual Meeting of the Japan Society of Water Environment, Gifu, Japan. (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakayama S.: "Measurement of a time-weighted average (TWA) concentration of benzo (a) pyrene in marine using improved blue rayon hanging technique."Abstract for 29th Annual Meeting of the Japan Environmental Mutagen Society, Sendai, Japan. (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nogami Y.: "Time-space distribution of heavy metals in mussel in the Seto Inland Sea, Japan"Abstract for PRIMO11th at Plymouth, UK, July. 11-13 (2001)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2002-03-26  

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