2001 Fiscal Year Final Research Report Summary
Analyois of infectivity in the blood from patients with prion disease
| Project/Area Number |
11557046
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Neurology
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| Research Institution | Tohoku University |
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Principal Investigator |
MURAMOTO Tamaki Tohoku University school of medicine, Research Associate, 大学院・医学系研究科, 助手 (40302096)
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| Co-Investigator(Kenkyū-buntansha) |
KITAMOTO Tetsuyuki Tohoku University school of medicine, Professor, 大学院・医学系研究科, 教授 (20192560)
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| Project Period (FY) |
1999 – 2001
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| Keywords | Creutzfeldt-Jakob disease / bioassay / follicular dendritic cells / blood / knock-in mouse / prions |
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| Research Abstract |
It is important to know whether the blood of a patient with Creuzfeldt-Jakob disease (CJD) is infectious or not. In fact, United Kingdom gave up to make the blood-derived products using the blood samples in United Kingdom because patient with variant CJD have extraneuronal abnormal prion protein (PrP^<Sc>). These accumulations were observed in the follicular dendritic cells (FDC) before the onset of variant CJD. It is an urgent issue to establish a sensitive bioassay system, because bovine spongiform encephalopathy is now appeared in almost all European countries, and even in Japan in 2001. Previous bioassay system, which is based on the intracerebral inoculation, showed a high sensitivity to detect infectivity of 10^<-7> diluted samples but took a long time (about 2 years) to detect the onset of disease. With FDC assay, the accumulated PrP^<Sc> consisted of recombinant PrP, but not of the inoculated human PrP. These accumulations were detectable in the spleens of all mice examined 30 days post-inoculation. Infectivity of the spleen was also evident. The FDC bioassay takes a short incubation time and shows a high sensitivity to detect the infectivity even in 10^<-7> diluted samples. This bioassay is also available to detect variant CJD prions. Therefore, we established a rapid and sensitive bioassay method for human prions. This model should facilitate the prevention of infectious prion diseases.
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Research Products
(17 results)
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[Publications] Yamada H., Itoh Y., Inaba A., Wada Y., Takashima M., Satoh S., Kamata T., Okeda R., Kayano T., Suematsu N., Kitamoto T., Otomo E., Matsushita M., Mizusawa H.: "An inherited prion disease with PrP P105L mutation: clinicopathological and PrP heterogeneity."Neurology. 53. 181-188 (1999)
Description
「研究成果報告書概要(欧文)」より
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