2000 Fiscal Year Final Research Report Summary
Novel immunotherapy for Hematological Malignancy
| Project/Area Number |
11557074
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Hematology
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| Research Institution | Nagoya University |
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Principal Investigator |
SAITO Hidehiko School of Medicine, Nagoya University, Professor, 医学部, 教授 (20153819)
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| Co-Investigator(Kenkyū-buntansha) |
EMI Nobuhiko School of Medicine, Nagoya University, Resarch Associate, 医学部, 助手 (30185144)
TANIMOTO Mitune School of Medicine, Nagoya University, Assistant Professor, 医学部, 講師 (10240805)
NAOE Tomoki School of Medicine, Nagoya University, Associate Professor, 医学部, 助教授 (50217634)
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| Project Period (FY) |
1999 – 2000
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| Keywords | Vaccine / GVL / SEREX / CEV / SYK / Gene Gun / DNA Vaccine |
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| Research Abstract |
We have isolated leukemia specific fusion genes, CEV14-PDGFRβ and TEL-Syk, and mutant FLT3 Receptor. Also sixteen genes were isolated from leukemia genes using SEREX methods. These gene products have immnogenic motif and antibody response.
DNA vaccination has emerged as an attractive approach for tumor immunotherapy. The technique of gold particle containing DNA bombardment by Herios Gene Gun (Bio-Rad) can be used to transfer genes to several tissues in vitro and in vivo. The aim of this study was to evaluate the efficiency of gene transfer in vitro and the potency of DNA vaccines in preventing and treating murine malignant tumor. We used the gene gun method to vaccinate C3H/He J mice intradermally with DNA vaccines containing the mutant FLT3R expression vector or the galactosidase expression vector in vivo. After immunization, mice were challenged intracutaneously on the abdomen with 1.5×10^7 tumorous 32D cells which transfected mutant FLT3R expression vector. Antitumor immunity was analyzed in both tumor prevention and tumor regression experiments. Mice immunized with mutant FLT3R expression vector prevented tumor and had antibody for tumor cells. In mice immunized with the galactosidase expression vector, tumor masses were palpable within 14 days after tumor challenge but disappeared. Control mice developed palpable and progressively growing tumors.
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[Publications] Zhao, M., Kiyoi, H., Yamamoto, Y., Ito, M., Towatari, M., Omura, S., Kitamura, T., Ueda, R., Saito, H.and Naoe, T.: "In viro treatment of mutant FLT3-transformed murine leukemia with a tyrosine kinase inhibitor."Lekemia. 14. 374-378 (2000)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Tomita, A., Towatari, M., Tsuzuki, S., Hayakawa, F., Kosugi, H., Tamai, K., Miyazaki, T., Kinoshita, T.and Saito, H.: "c-Myb acetylation at the carboxyl-terminal conserved domain by transcriptional co-activator p300."Oncogene. 19. 444-451 (2000)
Description
「研究成果報告書概要(欧文)」より
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