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2001 Fiscal Year Final Research Report Summary

Investigation of Pathogenetic Mechanisms of Spinal Cord Insury using Bcl-2/Bax Transgenic and Knockout Mice

Research Project

Project/Area Number 11557103
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Cerebral neurosurgery
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

HIDA Kazutoshi  Hokkaido Univ., Grad. Schoolof Med., Lecturer, 医学部・附属病院, 講師 (10238305)

Co-Investigator(Kenkyū-buntansha) 岩崎 喜信  北海道大学, 大学院・医学研究科, 教授 (00113522)
SEKI Toshitaka  Hokkaido Univ. Hosp., Dept. Neurosurg., Research Fellow, 医学部・附属病院, 医員
TADA Mitsuhiro  Hokkaido Univ., Institute for Genet. Med., Asso. Prof., 遺伝子病制御研究所, 助教授 (10241316)
Project Period (FY) 1999 – 2001
KeywordsSpinal Cord / Injury / Apoptosis / Bcl-2 / Bax / Transgenic Mice / Knockout Mice
Research Abstract

In this research project, we investigated roles of neuronal cell apoptosis (programmed cell death) secondary to the primary injury in aggravation of traumatic lesion in the spinal cord. For this purpose, we used transgenic/knockout mice of Bcl-2 family genes which are important in regulation of cellular apoptosis. We first established a mouse model of spinal cord injury using a pneumatic impact device and its neurological evaluating system. Applying the model system on Bcl-2 transgenic mice, we obtained the following results:
1) Post traumatic neurologic recovery showed a trend to be better in Bcl-2 transgenic mice than in wild-type mice
2) Lesion volume assessed by histological quantative evaluation showed a statistically significant difference between wild-type mice (5.1 ± 0.8 x 10^<-2> ml) and Bcl-2 transgenic mice (2.9±0.5x10^<-2> ml).
These results indicate that extension of traumatic lesion after a spinal cord injury is largely affected by the-anti- and pro-apoptotic effects of Bcl-2 family proteins. We therefore suggest that Bcl-2 family proteins can be important therapeutic targets in prevention of secondary damage in spinal cord injury.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Seki T, Hida K, et al.: "Graded contusion model for the mouse spinal cord using a pneumatic impact device"Neurosurgery. 50(印刷中). (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Seki T, Iwasaki Y et al.: "Anterior decompression of the atlantoaxial vertebral artery to treat bow hunter's stroke : technical case report"Neurosurgery. 49. 1474-1476 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Koyanagi I, Iwasaki Y, et al.: "Acute cervical cord injury without fracture or dislocation of the spinal column"J Neurosurg. 93. 15-20 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Seki T., Hida K., et al.: "Graded contusion model for the mouse spinal cord using a pneumatic impact device"Neurosurgery. 50 (in print). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Seki T., Iwasaki Y. et al.: "Anterior decompression of the atlantoaxial vertebral artery to treat bow hunter's stroke: technical case report"Neurosurgery. 49. 1474-1476 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Koyanagi I., Iwasaki Y., et al.: "Acute cervical cord injury without fracture or dislocation of the spinal column"J. Neurosurg.. 93. 15-20 (2000)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2003-09-17  

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