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2002 Fiscal Year Final Research Report Summary

Analysis for pathogenicity of periodontal disease-associated anaerobesin silent aspirated pneumoniae

Research Project

Project/Area Number 11557141
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 病態科学系歯学(含放射線系歯学)
Research InstitutionTOKYO DENTAL COLLEGE

Principal Investigator

OKUDA Katsuji  Tokyo Dental College, Department Dentistry, Professor, 歯学部, 教授 (40085741)

Co-Investigator(Kenkyū-buntansha) KIMIZUKA Ryuta  Tokyo Dental College, Department Dentistry, Assistant, 歯学部, 助手 (90287178)
ISHIHARA Kazuyuki  Tokyo Dental College, Department Dentistry, Assistant Professor, 歯学部, 助教授 (00212910)
KATO Tetsuo  Tokyo Dental College, Department Dentistry, Assistant Professor, 歯学部, 助教授 (00159253)
YAMANAKA Ayumi  Tokyo Dental College, Department Dentistry, Assistant, 歯学部, 助手 (40231667)
Project Period (FY) 1999 – 2002
Keywordssilent aspiration / pneumonia / elderly person / oral bacteria / mixed infection / Porphyromonas gingivalis / Treponema denticola / inflammatory cytokines
Research Abstract

Periodontal disease-associated bacteria have been reported to be the source of pathogens that not only cause oral infectious diseases but also are closely involved in systemic diseases. We examined the inflammatory responses against mixed infections of Porphyromonas gingivalis with T. denticola in bronchoalveolar lavage fluids (BALF).
After mixed infections with P. gingivalis and T. denticola into lung in mouse,we studied the viable cell numbers ofP, gingivalis cells and inflammatory responses in the BALF. Inflammatory cytokines, including interleukin 1/β (IL-1 /β), IL-6,tumor necrosis factor a. (TNF0), and mouse KC (human interleukin 8 like cytokine) were determined in BALF by ELISA systems specific for these cytokine molecules according to the manufacturer's recommended procedures.
When mice were inoculated with a mixture ofP. gingivalis at a dose of2.8 x10^8 and T, denticola., viable cell numbers P. gingivalis in the BALF steadily declined at 24, 48, and 72 h. The viable cell numbers ofP. gingivalis48 h and 72 h afterinoculation of a mixed suspension with T. denticola were significantly higher thanthose after monoinfection (P<0.05). The TNF 0 level in the BALF from infected mice progressively increased for the first 24 h after intratracheal inoculatiob and then decreased. The EL-1 Blevel in the BALF in mice with mixed infections at 24h after inoculation was significantly increased as compared with that after P. gingivalis monoinfection or T. denticola infection (P<0.05). The IL-1 B. level in theBALF in the mice with mixed infections decreased with time, butthe IL-6 level in the BALF in the same mice increased with time. The KC level in the BALF in the mice with mixed infection was significantly higher than that in those with P.gingivalis monoinfection at 48 and 72 h.
The present study demonstrates that a mixed inoculation of P. gingivalis withT. denticola causes a marked inflammatory response in mouse BALF and leids tolung abscesses.

  • Research Products

    (32 results)

All Other

All Publications (32 results)

  • [Publications] Kato T, Okuda K: "Actinobacillus actinomycetemcomitans possesses an antigen binding to anti-human IL-10 antibody"FEMS Microbiolo. Lett. 204. 293-297 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Adachi M. et al.: "Effect of professional oral health care on elderly living in nursing homes"Oral Sur. Oral Med. Oral Path. Oral Rad. Endodontics. 94. 191-195 (2002)

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      「研究成果報告書概要(和文)」より
  • [Publications] Kato T.et al.: "Salivary cystines induce interleukin-6 expression via cell surface molecules"Molecu.Immun.. 39. 423-430 (2000)

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  • [Publications] Eguchi A. et al.: "PCR method is essential for detecting Mycobacterium tuberculosis in oral cavity samples"Oral Microbiol.Immunol.. (in press).

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  • [Publications] Okuda K. et al.: "Ecological and immunopathological implications of oral bacteria in H.pylori-infected disease"Journal of Periodontology. (in press).

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  • [Publications] Okuda M. et al.: "Effect of mechanical and chemical oral cleansing on reducing potential -"J.Anestheology. (in press).

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  • [Publications] 奥田克爾: "新口腔微生物学-口腔内バイオフィルム感染症-"一世出版. 480 (2002)

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  • [Publications] Takihashi J, Saito A, Nakagawa T, Yamada S, Ishihara K and Okuda K.: "Dynamics of serum immunoglobulin G activity for Porphyromonasgingiva lis in adult periodontitis"J. Periodontol.. 69. 367-373 (1998)

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  • [Publications] Ishihara K, Kuramitsu H. K, Miura T and Okuda K.: "Deniilisin activity affects the organization of the outer sheath of Trcponema denticola"J. Bacteriol.. 180. 3837-3844 (1998)

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  • [Publications] Honma K, Kato T and Okuda K.: "Salivary immunoglobulin A production against asyntheticoligopeptide antigen of Actinobacillus actinomycetemcomitans fimbtriae"Oral Microbiol Immunol.. 14. 34-38 (1999)

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  • [Publications] Arai H, Xin K-Q, Hamajiraa K, Lu Y, Watabe S, Okuda K, Toda S,Tani K, Kudoh I, Suzuki M, and Okuda K: "8 Br-cAPM enhances both humoral and cell-mediated immuneresponses induced by an HIV-1 DNA vaccine"Gene Therapy. 7. 694-702 (2000)

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  • [Publications] Kusakabe K, Xin K-Q, Katoh H, Sumino K, Hagiwara E, Kawamoto S, Okuda K, Miyagi Y, Aoki A, Nishioka K, Kliman D and Okuda K.: "The timing of GM-CSF expression plasmid administration influences the ThlATh2 Response induced by an HIV-1 specific DNA vaccine"J. Immunol.. 164. 3102-31ll (2000)

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  • [Publications] Ishihara K, Ando T, Kato T, Moriraoto M, Yamane G, Takahashi S,Qgiuchi H and Okuda K.: "Relationship between the onset of pustulosis palmaris et plantaris,chronic oral infections, and bacterial heat shock proteins"Oral Microbiol. Immun.. 15. 232-237 (2000)

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  • [Publications] Okuda K, Ishihara K, Miura T, Kalakura A, Noma H. and Ebihara Y.: "Helicobacter pylori may have only transient presence in the oralcavity and the surface of oral cancer"Microbiol. Immun.. 44. 385-388 (2000)

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  • [Publications] Kato T, Honma K, Yamanaka A, Miura T and Okuda K.: "Heterogeneity in the immune response to serotype b LPS of Actinobacillus actinomyetemcomitans and in inbred strains of mice"FEMS Immunol. Med. Microbioi.. 28. 67-70 (2000)

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  • [Publications] Komiya A, Kato T, Nakagawa T, Saito A, Takahashi J, Yamada S and Okuda K.: "A rapid DNA probe method for detection of Porphyromonas gingiva lis and Actinobacillus actinomycetemcomitans"J. Periodonlol.. 71. 760-767 (2000)

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  • [Publications] Yoshinari M, Oda Y, Kato T, Okuda K and Hirayama A.: "Influence of surface modifications to titanium on oral bacterialadhesion in vitro."J. Biomed. Mater. Res.. 52. 388-394 (2000)

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  • [Publications] Imatani T, Kato T, Minaguchi K and Okuda K.: "Histatin 5 in saliva inhibits inflammatory cytokines induction from human gingival fibroblasts by Porphyromonas gingivalis"Oral Microbiol. Immun.. 15. 378-382 (2000)

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  • [Publications] Imatani T, Kato T and Okuda K.: "Production of inflammatory cytokines by human gingival fibroblasts stimulated by cell-surface preparations of Porphyromonas gingivalis"Oral Microbiol. Immun.. 16. 65-72 (2001)

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  • [Publications] Kato T, Imatani T, Miura T, Minaguchi K, Saitoh E and Okuda K.: "CytokJne inducing activity of family 2 cystatins"Biol. Chem.. 381. 1143-1147 (2001)

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  • [Publications] Abe S, Ishihara K and Okuda K.: "Prevalence of potential respiratory pathogens in the mouths of elderly patients and effects of professional oral care"Archs Gerontol. Gerialri.. 32. 45-55 (2001)

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  • [Publications] Fournier D, Mouton C, Lapierre T, Kafo,T. Okuda K and Menard C: "Porpyromonas gulae sp. nov., an anaerobic gram-negative coccobacillus from the gingival sulcus of various animal hosts"Int. J. Syst. Evolut. Microbiol.. 36. 252-257 (2001)

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  • [Publications] Okuda K, Ishihara K, Nakagawa T, Hirayama A, Inayama Y. and Okuda K.: "Detection of Treponeme denticola in atherosclerotic lesions"J. Clin. Microbiol.. 39. 1114-1117 (2001)

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  • [Publications] Yonezawa H. Ishihara K. and Okuda K.: "Arg-gingipain A DNA vaccine induced protective immunity againstinfection by Porphyromonas gingivalls in a murine model"Infect. Immun.. 69. 2858-2864 (2001)

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  • [Publications] Tadokpro K, Koizumi K, Miyagi Y, Kojima Y, Kawamoto S,Hamajjima K, Okuda K, Tanaka S, Onari K, Wahren B, Aoki I and Okuda K.: "Rapid wide -reaching delivery of HIV-1 env DNA vaccine by intranasal administration"Viral Immunol.. 14. 159-167 (2001)

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  • [Publications] Ishihara K, Miura T, Ebihara Y, Kamiya S, Hirayama T and Okuda K: "Shared antigenicity of Helicobacter pylori and periodontopathic Campyobacter rectus strains"FEMS Microb. Lett.. 197. 23-27 (2001)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Hirose M, Ishihara K, Saito A, Nakagawa T, Yamada S and Okuda K.: "Expressionof cylokines and inducible nitric oxide synthase in inflamed gingival tissue"J. Periodontol.. 72. 590-597 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kato T and Okuda K.: "Actinobacillus actinomycetemcomitans possesses an antigen binding to anti-human IL-10 antibody"FEMS Microbiolo. Lett.. 204. 293-297 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tadokoro K, Koizumi K, Miyagi Y, Kojima Y, Kawamoto S,Hamajima K, Okuda K,Tanaka S, Onari K, Wahren B, Aoki I and Okuda K.: "Rapid wide -reaching deliveryof HIV-1 env DNA vaccine by intranasai administration"Viral Immunol.. 14. 159-167 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] ishihara K, Miura T, Ebihara Y, Kamiya S, Hirayama T and Okuda K.: "Shared antigenicity of Helicobacter pylori and periodcjntopathic Campylobacter rectus strains"FEMS Microb. Lett.. 197. 23-27 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kato T and Okuda K.: "Actinobacillus actinomycetemcomitans possesses an antigen binding to anti-human IL-10 antibody"FEMS Microbiolo. Lett.. 204. 293-297 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Adachi M, Ishihara K, Abe S, Okuda K and ishikawa T: "Oral Effect of professional oral health care on elderly living in Nursing homes"Oral Sur. Oral Med. Oral Path. Rad. Endodontics. 94. 191-195 (2002)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2004-04-14  

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