Molecular Analysis for the involvment of Helicobacter Pylori in carcinogenis of oral cancer

2001 Fiscal Year Final Research Report Summary

• Project/Area Number: 11557159
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 展開研究
• Research Field: Surgical dentistry
• Research Institution: KYOTO UNIVERSITY
• Principal Investigator: IIZUKA Tadahiko Kyoto Univ., Faculty of Medicine, Professor, 医学研究科, 教授 (80026921)
• Co-Investigator(Kenkyū-buntansha): KUSUMOTO Takashi Kyoto Univ., Faculty of Medicine, Assistant Professor, 医学研究科, 助手 (10301251) ; YOKOE Yoshihiko Kyoto Univ., Faculty of Medicine, Assistant Professor, 医学研究科, 助手 (30211650) ; WAKATSUKI Yoshio Kyoto Univ., Faculty of Medicine, Assistant Professor, 医学研究科, 講師 (40220826)
• Project Period (FY): 1999 – 2001
• Keywords: Helicobacter pylori / oral cancer / oral lichen planus / Pradication

Research Abstract

Considering a suspected link between Helicobacter pylori infection and human oral cancer, we had cloned a new H.pylori gene for membrane protein 1 (HP-MP1) at first. Because HP-MP1 induces release of inflammatory cytokines and tumor necrosis factor-alpha acts as both initiator and tumor promoter, we studied the possible involvment of HP-MP1 in carcinogenesis of H.pylori. Two cell lines, BALB/3T3 cells as control and v-Ha-ras-transfected BALB/3T3 cells as putative initiated cells, were each transfected with HP-MP1, urease B genes, or vector alone. All of the Bhas/mpl clones showed strong expression of tumor necrosis factor-alpha gene and produced tumors in 100 % of nude mice. Two Bhas/ure clones showed weak tumorigenicity ; the other Bhas and BALB clones showed none. Results indicate strong carcinogenic activity of HP-MP1 in cooperation with viral Ras protein and weak activity of urease B.
Secondly, we often see the changes Oral lichen planus(OLP) for cancer. We found that 50 % of OLP patients infected with H.pylori. So, we analyzed the effect the eradication therapy for H.pylori. 5 OLP patients who had H.pylori infection treated with the eradication therapy. 3 of 5 patients were effective for 3 months, but 2 of 3 patients complaned pain 6 months later. Only one patient was effective for 12months. 2 of 5 patients were not effective at all. Thus, it is considerd that there is ho relationship between H.pylori infection and OLP.

Research Products

• [Publications] Shirai Y. et al.: "Induction and Maintenance of Immune effector Cells in the Gastric Tissue in Mice Orally Immunized to Helicobacter pylori Requires Salivary Glands"Gastroenteology. 118. 749-759 (2000)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Hiroaki Yoshida et al.: "MALT lymphoma of minoraltvary glad of the hard plate : a case report"Jpn Joral diag/oral med. 13. 542-546 (2000)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Suganuma M. et al.: "Helicobacter pylori Membrane Protein 1 : A new carcinogenic Factor of Helicobacter pylori"Cancer Research. 61. 6356-6359 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Yoshida M. et al.: "CD4T cells monospecific to ovalbumin produced by Escherychia colt cantnduce colitis upon transfer to BALBl and SCID mace"Int. Immurol.. 13. 1561-1570 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Watanabe T. et al.: "Administration of an antigen at a high dose generates Regulatory CD4^+ T Cells Expressing CD95 Ligand and Secreting IL-4 in the Liver"Journal of immunology. 168. 2188-2199 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Yoshida M. et al.: "Differential localization of colitogenic Th1 and Th2 cells monospecific to a microflola-associated antigen in mice"Gastroenterology. 123. 1949-1961 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Shirai Y., Wakatsuki Y., Kusumoto T., Nakata M., Yoshida M., Usui T., Iizuka T., Kita T.: "Induction and maintenance of immune effect or cells in the gastric tissue of mice orally immunized to Helicobacter pylori requires salivary glands"Gastroent erology. 118(4). 749-59 (2000)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Hiroaki Yoshida, Shigeyuki Fujita, Yoshiaki Fukumura, Yasuhiko Shirai, Ken-ichiro Murakami, Tadahiko Iizuka: "MALT lymphoma of minor alivary gland of the hard plate : a case report"Jpn J oral diag/oral med.. 13(2). 542-546 (2000)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Suganuma M.,k Kurusu M., Okabe S., Sueoka N., Yoshida M., Wakatsuki Y., Fujiki H.: "Helicobacter pylori membrane protein 1 : a new carcinogenic factor of Helicobacter pylori"Cancer Research. 61(17). 6356-9 (2001)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Yoshida M., Watanabe T., Usui T., Matsunaga Y., Shirai Y., Yamori M., Itoh T., Habu S., Chiba T., Kita T., Wakatsuki Y.: "CD4 T cells monospecific to ovalbumin produced by Escherichia coli can induce colit is upon transfer to BALB/c and SCID mice"Int. Immunol.. 13(12). 1561-70 (2001)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Watanabe T., Yoshida M., Shirai Y., Yamori M., Yagita H., Itoh T., Chiba T., Kita T., Wakatsuki Y.: "Administration of an antigen at a high dose generates Regulatory CD4+ T cells expressing CD95 ligand and secreting IL-4 in the liver"Journal of Immunology. 168(5). 2188-99 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Yoshida M., Shirai Y., Watanabe T., Yamori M., Iwakura Y., Chiba T., Kita T., Wakatsuki Y.: "Differential localization of colit ogenic Th1 and Th2 cells monospecific to a microflora-associated antigen in mice"Gastroent erology. 123(6). 1949-61 (2002)
  • Description: 「研究成果報告書概要(欧文)」より