2000 Fiscal Year Final Research Report Summary
Development of detection method of metastasis from squamous cell carcinoma of head and neck using peripheral blood
| Project/Area Number |
11557163
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Surgical dentistry
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| Research Institution | Showa University |
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Principal Investigator |
NAGUMO Masao Showa university, School of Dentistry, Professor, 歯学部, 教授 (70013993)
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| Co-Investigator(Kenkyū-buntansha) |
HATORI Masashi Showa University, School of Dentistry, lecturer, 歯学部, 講師 (20245814)
IWASE Masayasu Showa University, School of Dentistry, lecturer, 歯学部, 講師 (50193743)
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| Project Period (FY) |
1999 – 2000
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| Keywords | oral cancer / metastasis / magnetic bead / circulating cancer cell / PT-PCR / CD44 / Cytokeratin 19 / MMP |
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| Research Abstract |
We compared the expression of cytokeratin 19 (CK19), CD 44, and its variant forms (s, v6, v9) mRNAs on primary oral squamous carcinoma cells and their micrometastatic cancer cells in peripheral blood by using RT-PCR method. The micrometastatic cancer cells were collected by an immunomagnetic separation technique and the primary cancer cells were obtained by a laser-captured microdissection method.
The results obtained were as follows :
1. Fifty cancer cells in 5 ml of blood could be captured by immunobeads coated with specific antibody for human epithelial antigen.
2. The expression of CK19 mRNA was detected in about half of the primary and circulating cancer cells.
3. CD44 mRNA was not expressed on the primary cancer cells, whereas most of cancer cells in peripheral blood expressed mRNA of CD44 and its variant forms.
4. MMP (2, 7, 9) mRNAs were expressed on the primary cancer cells, but they were not usually expressed on circulating cancer cells.
5. Metastatic lesions (lung, lymph node) were made by transplanting tongue cancer cell lines (SCCC, SAS or N2NA) into nude rats and the expression of above-mentioned mRNAs on cells in primary and metastatic lesions are now being examined.
These results indicate that the gene expression might be different between primary and circulating cancer cells.
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