2000 Fiscal Year Final Research Report Summary
Gene target therapy against human bladder cancer by gelsolin gene
| Project/Area Number |
11557187
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
医薬分子機能学
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| Research Institution | Hokkaido University |
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Principal Investigator |
KUZUMAKI Noboru Hokkaido University, Institute For Genetic Medicine, Professor, 遺伝子病制御研究所, 教授 (80091445)
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| Co-Investigator(Kenkyū-buntansha) |
SHINOHARA Nobuo Hokkaido University, School Of Medicine Hospital, Lecturer, 医学部・附属病院, 講師 (90250422)
FUJITA Hisakazu Hokkaido University, Institute For Genetic Medicine, Instructor, 遺伝子病制御研究所, 助手 (30212187)
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| Project Period (FY) |
1999 – 2000
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| Keywords | gelsolin / bladder cancer / gene therapy / adenovirus / nude mice / β-gal |
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| Research Abstract |
We originated and tested recombinant adenovirus encoding wild-type gelsolin (Ad-GSN) to determine the efficacy of gelsolin overexpression as a therapeutic reagent in an orthotopic model of nude mice. This model provides a microenvironment similar to that of postsurgical bladder cancer. In vitro, an inhibitory effect on the growth of human bladder cancer cell line KU-7 infected with Ad-GSN was confirmed. Flow cytometric analysis revealed this effect may be occurred by cell cycle arrest or delay at G2/M.In this orthotopic model, KU-7 cells were introduced into the bladder of nude mice, and 1×10^9 PFU of Ad-GSN or control adenovirus was injected via the urethra for three days. After 10 days the total area of tumor masses in 20 sections of each bladder was digitally determined after staining. In Ad-GSN treated bladder, approximately 90% of tumor growth was inhibited compared to controls. These findings suggest that the expression of gelsolin using adenovirus vector may be useful as a therapeutic reagent against bladder cancer.
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