2001 Fiscal Year Final Research Report Summary
The method for determination of serum lipid-free apolipoprotein concentration and its clinical application
| Project/Area Number |
11557204
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Laboratory medicine
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| Research Institution | Gifu University |
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Principal Investigator |
SEISHIMA Mitsuru Gifu University, Department of Laboratory Medicine, Professor, 医学部, 教授 (10171315)
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| Co-Investigator(Kenkyū-buntansha) |
WADA Hisayasu Gifu University, Department of laboratory Medicine, Lecturer, 医学部, 助手 (10283300)
SAITO Kuniaki Gifu University, Department of Laboratory Medicine, Assistant Professor, 医学部・附属病院, 講師 (80262765)
MINATOGUCHI Shinya Gifu University, 2nd Department of Internal Medicine, Associate Professor, 医学部, 助教授 (20190697)
SAITO Kazunori Daiichi Pure Chemicals Company, Research Manager, 診断薬研究所, リサーチマネージャー
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| Project Period (FY) |
1999 – 2001
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| Keywords | Lipid-free-free apolipoprotein / Corobary heart disease / Atherosclerosis / pre βHDL |
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| Research Abstract |
It is known that HDL are heterogeneous in particle size and small HDL particle can promote more effectively cholesterol efflux from cell membrane than large HDL particle. Small HDL are detected by two-dimentional gel electrophoresis, but this method is time consuming. Lipid-free apo A-I in this study was separated by HPLC and was elutred from 51 min to 56 min. The epitope of lipid-free apo A-I is the same as tjhat of a HDL, because we used the antibody to apo A-I in the ELISA system. We added SOU/ml of trasyrol to blood and also to 500 μ1 of fractionated solutions by HPLC. Serum lipid-free apo A-I concentration increased two times by repeated freeze and thawing test and changed with time even in the store at 4℃. Thus, serum sample was immediately treated for HPLC or freezed at -80℃ until use.
Serum lipid-free apo A-I concentrations significantly increased in patients with coronary heart disease compared to healthy controls in both males and females. These findings show that reverse cholesterol transport is altered in coronary heat disease and the measurement of lipid-free apo A-I may be a good marker for atherosclerosis.
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