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2001 Fiscal Year Final Research Report Summary

Morphological and ultrastructural changes of the membrane systems involved in excitation-contraction coupling in skeletal muscle during contraction

Research Project

Project/Area Number 11558003
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 体育学
Research InstitutionNational Institute of Fitness and Sports in Kanoya

Principal Investigator

TAKEKURA Hiroaki  National Institute of Fitness and Sports in Kanoya, Physiological Sciences, Professor, 体育学部, 教授 (00206963)

Co-Investigator(Kenkyū-buntansha) KASUGA Norikatsu  Aichi University of Education, Phisical Education, Professor, 教育学部, 教授 (60152659)
Project Period (FY) 1999 – 2001
Keywordsskeletal muscle / excitation-contraction coupling / sarcoplasmic reticulum / transverse tubules / exercise / triad / three-dimensional structure / electron microscope
Research Abstract

During excitation-contraction (e-c) coupling in skeletal muscle, depolarization of the surface membrane/transverse (T) tubule is converted into Ca^<2+> release from internal store, sarcoplasmic reticulum (SR). These two membrane systems make junctions with each other, named peripheral coupling, dyads, and triads or, collectively, calcium release units. Functional interaction between T tubule and SR involves two Ca^<2+> channels in these membrane complex: dihydropyridine receptors (DHPR) of surface membrane/transverse tubules, and ryanodine receptor (RyR) of SR. The steps leading to the differentiation of the specialized SR domain involved in CRUs are just beginning to be understood. Key to the formation of CRUs is a docking step, which allows the formation and stabilization of the SR-T tubule link. Candidates for the docking protein have been proposed. Another step is the insertion of RyRs and DHPRs within CRUs. The former can be followed using electron microscopy, since the cytoplasmic domains of RyRs are seen as well defined electron dense structures within the junctional gap between SR and plasmalemma/T tubules. Using the mouse diaphragm as a model, we report further insights into the morphogenesis of the membrane systems. We found that the SR-T docking and the trapping of RyRs at the junctions are two sequential steps in the formation of CRUs. A second, somewhat unexpected, finding is that the specific positioning of CRUs relative to the bands of the myofibrils proceeds positioning of the entire T tubule network in transverse planes associated with the myofibrils. We found that the SR-T docking and the trapping of RyRs at the junctions are two sequential steps in the formation of GRUs. These sequential stages suggest an order of inductive processes for the molecular differentiation and structural organization of the CRUs in skeletal muscle development.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Takekura, H.: "Sequential docking, molecular differentiation, and positioning of T-tubule/SR junctions in developing mouse skeletal muscle"Dev. Biol.. 239. 204-214 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takekura, H.: "Eccentric exercise-induced morphological changes in the membrane systems involved in excitation-contraction coupling in rat skeletal muscle"J. Physiol.. 533. 571-583 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Protasi, F.: "RyR1 and RyR3 gave different roles in the assembly of calcium release units of skeletal muscle"Biophys. J.. 79. 2494-2508 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takekura, H.: "Correct targeting of dihydropyridine receptors and triadin in dyspedic mouse skeletal muscle in vivo"Dev. Dynamics. 214. 372-380 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takekura, H.: "Differential response of the membrane systems involved in excitation-contraction coupling to early and later postnatal denervation in rat skeletal muscle"J. Muscle Res. Cell Motility. 20. 279-289 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 竹倉広明: "筋収縮の最近の知見〜興奮収縮連関の機能を担う筋細胞内膜系とCa^<2+>チャンネル〜"体力科学. 48. 315-326 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 竹倉広明(分担執筆): "運動分子生物学"大日方 昴(監修),山田 茂・後藤勝正(編集) 有限会社ナップ(発行). 305 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takekura,H: "Sequential docking, molecular differentiation and positioning of T-Tubule/SR junctions in developing mouse skeletal muscle"Dev. Biol.. 239. 204-214 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takekura,H: "Eccentric exercise-induced morphological changes in the membrane systems involved in excittaion-contraction coupling in rat skeletal muscle"J. Physiol.. 533. 571-583 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Protasi,F.: "RyR1 and RyR3 have different roles in the assembly of calcium release units of skeletal muscle"Biophys. J.. 79. 2494-2508 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takekura,H.: "Correct targeting of dihydropyridine receptors and triadin in dyspedic mouse skeletal muscle in vivo"Dev. Dynamics. 214. 372-380 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takekura,H.: "Differential response of the membrane systems involved in excitation-contraction coupling to early and later postnatal denervation in rat skeletal muscle"Muscle Res. Cell Motility. 20. 279-289 (1999)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2003-09-17  

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