2000 Fiscal Year Final Research Report Summary
Establishment of novel techniques for manipulating mammalian fetuses at the molecular level
| Project/Area Number |
11558090
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Tohoku University |
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Principal Investigator |
OSUMI Noriko Tohoku University, Graduate School of Medicine, Professor, 大学院・医学系研究科, 教授 (00220343)
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| Co-Investigator(Kenkyū-buntansha) |
WAKAMATU Yoshio Tohoku University Graduate School of Medicine, Lecturer, 大学院・医学系研究科, 講師 (60311560)
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| Project Period (FY) |
1999 – 2000
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| Keywords | Neural development / Mammalian embryo / fetus / Manipulation / Ultrasound microscope / In utero operation / Gene transfer / Cortex / GFP |
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| Research Abstract |
Brain functions such as memory, lerning, and emotion, are based on neuronal networks connected among various neurons in specific regions. In order to elucidate molecular mechanisms of brain development, it is required to establish novel techniques to directly manipulate mammalian fetuses of the middle gestation and thereafter at molecular levels. This research project aimed to do the following subjects :
1) Establishment of techniques for manipulating and maintaining mammalian fetuses at the middle gestation and thereafter
2) Establishment of techniques for transplanting cells into the brain of mammalian fetuses at the middle gestation and thereafter
3) Establishment of techniques for transferring exogenous genes into the brain of mammalian fetuses at the middle gestation and thereafter
The following results are obtained in the period supported by this grant :
1) As a technique for manipulating and maintaining mammalian fetuses at the middle gestation and thereafter, we established ultrasound-guided in utero manipulation, thereby operated fetuses being able to survive and develop at the full term.
2) As preliminary experiments for cell transplantation and gene transfer, we tried to inject dye solutions into the operated fetuses.
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Research Products
(19 results)
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[Publications] Suzuki,R.,Shintani,T.,sakuta,H.,Kato,A.,Ohkawara,T.,Osumi,N.,. et al.: "Identification of RALDH-3, a novel retinaldehyde dehydrogenase, expressed in the ventral region of the retina."Mechanisms of Development. 98. 37-50 (2000)
Description
「研究成果報告書概要(和文)」より
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[Publications] Nagase,T.,Shimoda,Y.,Sanai,Y.,Nakamura,S.,Harii,K.,Osumi,N.: "Differential Expression of Two Glucuronyltransferases Synthesizing HNK-1 carbohydrate epitope in the sublineages of the rat myogenic progenitors."Mechanisms of Development. 98. 145-149 (2000)
Description
「研究成果報告書概要(和文)」より
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[Publications] Inoue,T.,Tanaka,T.,Takeichi,M.,Chisaka,O.,Nakamura,S.,Osumi,N.: "The role of cadherins in maintaining the compartment boundary between the cortex and striatum during development ."Development. 128. 561-569 (2000)
Description
「研究成果報告書概要(和文)」より
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[Publications] Kawano, H., Fukuda, T., Kubo, K., Horie, M., Takeuchi, K., Osumi, N., Eto, K., and Kawamura, K.: "Pax6 is required for the thalamocortical pathway formation in fetal rats."J.Comp.Neurol.. 408. 147-160 (1999)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Shimoda, Y., Tajima, Y., Nagase, T., Harii K., Osumi, N., and Sanai, Y.: "Cloning and expression of a novel galactoside b1,3-glucuronyltransferase involved in the biosynthesis of HNK-1 epitope."J.Biol.Chem.. 274. 17115-17122 (1999)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Akamatsu, W., Okano, H,J., Osumi, N., Inoue, T., Nakamura, S., Sakakibara, S., Miura, M., Matsuo, N., Darnell, R.B.and Okano, H.: "Mammalian ELAV-like neuronal RNA-binding proteins HuB and HuC promote neuronal development in both the central and the peripheral nervous systems."Proc Natl Acad Sci USA.. 96(17). 9885-90 (1999)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Tomioka, N., Osumi, N., Sato, Y., Inoue, T., Nakamura, S., Fujisawa, H., and Hirata, T.: "Neocortical origin and tangenitial migration of guidepost neurons In the lateral olfactory tract."J.Neurosci.. 20(15). 5802-5812 (2000)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Yamamoto, H., Ochiya, T., Takahama, Y., Ishii, Y., Osumi, N., Sakamoto, H., Terada, M.: "Detection of spatial localization of Hst-Fgf-4 gene expression in brain and testis from adult mice."Oncogene. 19. 3805-3810 (2000)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Suzuki, R., Shintani, T., Sakuta, H., Kato, A., Ohkawara, T., Osumi, N., Noda, M.: "Identification of RALDH-3, a novel retinaldehyde dehydrogenase, expressed in the ventral region of the retina."Mech Dev. 98. 37-50 (2000)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Nagase, T., Shimoda, Y., Sanai, Y., Nakamura, S., Harii, K., Osumi, N.: "Differential Expression of Two Glucuronyltransferases Synthesizing HNK-1 carbohydrate epitope in the sublineages of the rat myogenic progenitors."Mech Dev. 98. 145-149 (2000)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Inoue, T., Tanaka, T., Takeichi, M., Chisaka, O., Nakamura, S., & Osumi, N.: "The role of cadherins in maintaining the compartment boundary between the cortex and striatum during development."Development. 128. 561-569 (2001)
Description
「研究成果報告書概要(欧文)」より
