2000 Fiscal Year Final Research Report Summary
Mechanical stimuli to cultured cells with an optical manipulation system
| Project/Area Number |
11558116
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Kawasaki College of Allied Health Professions |
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Principal Investigator |
KATAOKA Noriyuki Kawasaki College of Allied Health Professions, Medical Engineering, Assistant Professor, 臨床工学科, 講師 (20250681)
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| Co-Investigator(Kenkyū-buntansha) |
KAJIYA Fumihiko Okayama University Medical School, Physiology II, Professor, 医学部, 教授 (70029114)
MOCHIZUKI Seiichi Kawasaki College of Allied Health Professions, Medical Engineering, Associate Professor, 臨床工学科, 助教授 (60759596)
GOTO Masami Kawasaki College of Allied Health Professions, Medical Engineering, Professor, 臨床工学科, 助教授 (50148699)
TSUJIOKA Katsuhiko Kawasaki Medical School, Physiology, Professor, 医学部, 教授 (30163801)
OGASAWARA Yasuo Kawasaki Medical School, Medical Engineering, Associate Professor, 医学部, 助教授 (10152365)
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| Project Period (FY) |
1999 – 2000
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| Keywords | optical manipulation / cultured cells / mechanical stimuli / mechano-sensing mechanism |
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| Research Abstract |
1. System setup : The optical manipulation system for mechanical stimulation to the cultured cells was built up. The 2.5 W and 1064nm wave length YAG laser was used as a laser source. The laser was split into two beams and the positions of the focus were controlled separately by the piezo actuator. Moreover, the confocal laser scanning system was combined with the optical manipulation system. We confirmed that the glass bead with 1μm diameter could be trapped and manipulated under the optical observation.
2. The responses of cultured endothelial cells to the mechanical stimuli : The nucleus of cultured bovine aortic endothelial cells was trapped with the optical tweezers and moved in a XY plane, and the change of cytosolic calcium ion was observed with a confocal laser scanning system. After the nucleus was moved, the increase of cytosolic calcium ion was observed. In addition, the calcium ion propagation from the stimulated cell to the neighboring cells. It is considered that endothelial cells may response to the micro-strain inside the cells. And the calcium ion propagation may be related with the cell-to-cell signal transduction, e.g. the formation of the gap junction between cells.
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Research Products
(11 results)
