• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2001 Fiscal Year Final Research Report Summary

New approach for drug monitoring by using human sweat secreted

Research Project

Project/Area Number 11559008
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 広領域
Research InstitutionNagoya Institute of Technology

Principal Investigator

TSUDA Takao  Nagoya Institute of Technology, Prof., 工学部, 教授 (50023156)

Co-Investigator(Kenkyū-buntansha) ISHII Akira  Nagoya University School of Medicine, Associate Prof., 医学部, 助教授 (30252175)
SUZUKI Osamu  Hamamatsu University School of Medicine, Prof., 法医学講座, 教授 (70093044)
Project Period (FY) 1999 – 2001
KeywordsSweat / drug / drug monitoring / noninvasive / theophylline / metabolites / easy sampling / monitoring at bed side
Research Abstract

Human sweat is one of the body fluids. Human sweat is secreted on a skin surface, so that it is easy to collect non-invasively. We intend to collect human sweat by handy sampling method and to use it for drug monitoring. First we developed the handy sampling method of sweat secreted and simultaneously we measured the amount of sweat. The handy method of sampling was archived by using a small vial (inner volume 0.6 ml) containing few percentage alcohol-water solution. The vial attached on the surface of human skin for 3 to 5 min for collecting sweat. Then the component in sweat was analyzed by liquid chromatograph, capillary electrophoresis, GC/MS and etc.
1.The variation of caffeine in sweat was measured after intake of a tablet containing caffeine. Caffeine and its metabolites were found in sweat and their concentrations were varied according to the elapsed period.
2. Codeine was also found after intake of a medicine.
3. Organic carboxylic acids and glucose were founded in sweat and their concentration variation were also studied after their intakes.
4. Volatile organic gases are directly collected from skin surface. The gases was led to cold trap for 30 second, and switched to analysis by gas chromatography. New devices of trapping has been investigated and applied for international patents.
5. Inorganic anions also investigated.
From above studies, we concluded it is possible to use sweat for drug monitoring, because it contains a lot of components that have a certain relationship with the concentration in blood. The use of sweat is very attractive because it is easy to adopt for testing of body conditions by people for their control of daily life.

  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Ken Naitoh: "Direct Temperature-controlled Trapping system and its use for the gas chromatographic determination of Organic vapor released from human skin"Analytical Chemistry. 72. 2797-2801 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takao Tsuda: "Proposal of sampling process for collecting human sweat and determination of caffeine concentration in it by using GC/MS"Biomedical Chromatography. 14. 505-510 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 津田孝雄: "カフェイン錠剤服用後の-汗中カフェイン及びその代謝物の存在確認とそれらの濃度経時変化"発汗学. 8. 13-19 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takao Tsuda: "Noninvasive approach for study of human reaction and transferring velocity of nervous instrumentation devised for concurrent microscopic observation and analysis of sweat secretion on human body under 1G and microgravity"Journal of gravitational Physiology. 7. 129-130 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 増村年章: "精神疾患患者におけるボールキャッチ時の発汗反応"発汗学. 7. 85-88 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大桑哲男: "運動負荷中の汗中乳酸量のモニタリング"発汗学. 8. 47-49 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 寺澤純子: "キャピラリー電気泳動法によるヒト汗中のナトリウム、カリウム、カルシュム、マクネシュム、アンモニウムイオンの定量"分析化学. 50. 813-817 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ken Naitoh: "New measurement of hydrogen gas and acetone in gases emanating from human skin"Instrumental Science & Technology. (in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 増村年章: "精神分裂症におけるボールキャッチ時の発汗反応-健常対照者との比較-"発汗学. 9. 25-28 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] "Exhalation behavior of four organic substrates and water absorbed by human skin"Biological & Pharmaceutical Bulltin. (in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takaharu Kondou: "Assessment of colonic fermentation by hydrogen release from skin"The American J. Gastroenterology. (in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 津田孝雄: "楽しい化学実験"丸善. 154 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 津田孝雄: "ヒトの発汗活動と汗に含まれる化学物質から分かること(化学への招待)"日本化学会東海支部化学教育協議会. 16-29 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ken Naitoh, Yoshihito Inai, Tadamichi Hirabayashi, and Takao Tsuda: "Direct Temperature-controlled Trapping system and its use for the gas chromatographic determination of Organic vapor released from human skin"Analytical Chemistry. 72. 2797-2801 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takao Tsuda, Saeko Noda, Shinya Kitagawa, and Toru Morishita: "Proposal of sampling process for collecting human sweat and determination of caffeine concentration in it by using GC/MS"Biomedical Chromatography. 14. 505-510 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takao Tsuda, Naohiro Naito, Nao Mizuno, Shinya Kitagawa, and Akira Ishii: "Determination of Caffeine and its metabolites in sweat secreted after intake of caffeine, and their time variation (in Japanese)"Japanese Journal of perspiration. 8. 13-19 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Tsuda, S.Kitagawa, and S.Nagaoka: "Noninvasive approach for study of human reaction and transferring velocity of nervous instrumentation devised for concurrent microscopic observation and analysis of sweat secretion on human body under 1G and microgravity"Journal of gravitational Physiology. 7. 129-130 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tetsuo Okuwa, Sadaharu Sugase, Youhei Tachi, Takako Yamamoto, Shinya Kitagawa, and Tsuda Takao: "Monitoring of concentration of lactic acid in human sweat secreted during excersice by using a newly devised-handy sampling"Japanese Journal of perspiration. 8. 47-49 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Junko Terasawa, Kotaro Mitsui, Akira Ishii, Takao Tsuda: "Quantitative determination of K^+, NH4^+, Ca^+, and Mg^+ cations in secreted human sweat by capillary zone electrophoresis"Bunseki Kagaku. 50. 813-817 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ken Naitoh, Yoshiki Inai, Tadamichi Hirabayashi, Takao Tsuda: "Exhalation behavior of four organic substrates and water absorbed by human skin"Biological & Pharmaceutical Bulletin. in press.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ken Naitoh, Takao Tsuda, Kazutodshi Nose, Takaharu Kondo, Akinori Takasu, and Tadamichi, Hirabayashi: "New measurement of hydrogen gas and acetone in gases emanating from human skin"Instrumental Science & Technology. in press.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takaharu Kondou, Takao Tsuda, Hirotoshi Ishiguro, Takahiro Mitsui, Kai-Ping Gao, and Kotoya Fujiki: "Assessment of colonic fermentation by hydrogen release from skin"The American J. Gastroenterology. in press.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hideki Shibata, Takao Tsuda: "Determination of paraben found on skin surface of human finger"Bunseki Kagaku. in press.

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2003-09-17  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi