2001 Fiscal Year Final Research Report Summary
Re-examination on two different biosynthetic pathways of galactolipids in higher plants using molecular cell biological technique.
| Project/Area Number |
11640644
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物生理
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
OHTA Hiroyuki Tokyo Institute of Technology, Associate Professor, 大学院・生命理工学部, 助教授 (20233140)
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| Project Period (FY) |
1999 – 2000
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| Keywords | Chloroplast / Glycolipid / Glycosyltransferase / Thylakoid / Monogalactosyldiacylglycerol |
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| Research Abstract |
1) Three different types of MGDG synthase genes (AtMGD1, AtMGD2, AtMGD3) were identified in Arabidopsis thaliana.
2) Tissue specificity of gene expression for three MGDG synthase genes was determined.
3) Enzymatic property of three MGDG synthases was determined.
4) Subcellular localization of three MGDG synthases was determined.
5) According to the results described above, we classified three MGDG synthases into two types, namely type A (AtMGD1) and type B (AtMGD2, AtMGD3). Role of the two types in two different biosynthetic pathways in galactolipid synthesis was discussed, and their distinct roles were proposed.
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[Publications] Awai, K., Marechal, E., Block, M.A, Brun, D., Masuda, T., Shimada, H., Takamiya, K., Ohta, H., Joyard, J.: "Two types of MGDG synthase genes, found widely in both 16:3 and 18:3 plants, differentially mediate galactolipid syntheses in photosynthetic and nonphotosynthetic tissues in Arabidopsis thaliana"Proc. Natl. Acad. Sci., USA. 98(19). 10960-10965 (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] Gad, M., Awai, K., Shimojima, M., Yamaryo, Y., Shimada, H., Masuda, T., Takamiya, K., Ikai, A., Ohta, H.: "Accumulation of plant galactolipid affects cell morphology of Escherichia coli"Biochem. Biophys. Res. Comm.. 286. 114-118 (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] Yamaryo, Y., Kanai, D., Awai, K., Masuda, T., Shimada, H., Takamiya, K., Ohta, H.: "Transcriptional regulation by light and phytochromes of the MGD gene in cucumber"Biochem. Soc. Trans.. 28. 738-740 (2000)
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「研究成果報告書概要(欧文)」より
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