2000 Fiscal Year Final Research Report Summary
LIGHT REGULATION OF BRASSINOSTEROID BIOSYNTHESIS
| Project/Area Number |
11640661
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物生理
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| Research Institution | RIKEN |
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Principal Investigator |
SHIMADA Yukihisa RIKEN, Plant Science Center, Scientist, 生長制御物質研究チーム, 研究員 (30300875)
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| Co-Investigator(Kenkyū-buntansha) |
NAKANO Takeshi RIKEN, Plant Functions Lab., Scientist, 植物機能研究室, 研究員 (30281653)
ASAMI Tadao RIKEN, Plant Functions Lab., Scientist, 植物機能研究室, 副主任研究員 (90231901)
FUJIOKA Shozo RIKEN, Plant Functions Lab., Scientist, 植物機能研究室, 先任研究員 (60165355)
NAGATA Noriko RIKEN, Plant Functions Lab., Scientist, 植物機能研究室, 基礎科学特別研究員 (40311352)
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| Project Period (FY) |
1999 – 2000
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| Keywords | brassinosteroid / P450 / environmental response / plant hormone / light regulation / regulation of biosynthesis |
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| Research Abstract |
Light has been suggested to be a negative regulator of brassionsteroid biosynthesis, but there have been no evidence both at biochemical and molecular biological level. We isolated brassinosteroid-6-oxidase gene (B6OX) from Arabidopsis. B6OX gene was functionally expressed in yeast and the enzyme converted [2H6]6-DeoxoTE to [2H6]CS, [2H6]TY and [2H6]TE respectively, but did not convert [2H6]6-deoxo3DT, [2H6]6-DeoxoCT and [2H6]CN.When transcript level of B6oX gene was investigated by northern analysis, it was higher in plants grown in light than in darkness. We also analyzed endogenous BRs for light- and dark-grown seedlings by GC-MS.Light-grown plants accumulated more C6-oxidized brassinosteroids than dark-grown ones.
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[Publications] Shimada, Y., Fujioka, S., Miyauchi, N., Kushiro, M., Takatsuto, S., Nomura, T., Yokota, T., Kamiya, Y., Bishop, G.and Yoshida, S.: "Brassinosteroid-6-oxidases from Arabidopsis and tomato catalyze multiple C-6 oxidations in brassinosteroid biosynthesis"Plant Physiol. 125(in press). (2001)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Asami, T., Min, Y., Sekimata, K., Shimada, Y., JM, W., Fujioka, S.and Yoshida, S.: "Mode of action of Brassinazole ; a specific inhibitor of brassinosteroid biosynthesis."ACS Symposium series. 774. 269-280 (2000)
Description
「研究成果報告書概要(欧文)」より
