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2001 Fiscal Year Final Research Report Summary

Physiological meaning of calcitonin mRNA found in the gonads of a teleos

Research Project

Project/Area Number 11640662
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 生物形態・構造
Research InstitutionKanazawa University

Principal Investigator

SASAYAMA Yuichi  Fac. Of Sci,. Kanazawa Univ., 理学部, 教授 (30018999)

Co-Investigator(Kenkyū-buntansha) NAKAMURA Masaru  Tropical Biosphere Research Center, Univ. of Ryukyus, Prof., 熱帯生物圏研究センター, 教授 (10101734)
SUZUKI Nobuo  Fac. Of Sci,. Kanazawa Univ. Assist. Prof., 理学部, 助手 (60242476)
Project Period (FY) 1999 – 2001
Keywordscalcitonin / mRNA / teleost eggs
Research Abstract

Researchers in US found the calcitonin (CT) mRNA in the uterus of rats, and supposed that CT may make a suitable environment of Ca for cellular adhesions and differentiation of organs in eiobryos. We were stimulated by their report, and tried to amplify the CT cDNA in the testes and ovaries of goldfish by RT-PCR method. As a result, the same sequence of the nucleotides as the ultimobranchial calcitonin was amplified from homogenates of those gonads. Furthermore, we found CT-immurioreactive axons of neurons in the testis of goldfish. Therefore, we planed to examine the access points of the axons of the CT-producing neurons, using tilapia, because those axons may be terminated at muscles or steroid-producing cells in the gonads. In addition, we were going to ablate the neural ganglion in the gonads, and to observe the effects. In tilapia, however, the neural ganglion was not immunoreacted against the anti-CT antiserum, and was not compact but dispersed. Therefore, this project was judged … More to be impossible to do, although we are regretted very much. However, we stuck at the expression of CT in gonads, and examined the presence of CT mRNA in salmon eggs. As a result, we could amplify CT cDNA by RT-PCR method in the egg membrane just ovulated and on the way,of embryo-developing. The amplification rate was larger in the animal pole side than that in the vegetal pole side. Furthermore, CT cDNA could be amplified from yolk as well. The pattern was similar to that in the egg membrane. Although we amplified the elongation factor as the control, the results were similar. Therefore, we could not conclude that distribution pattern of the CT mRNA is specific or not. In the egg membrane just after hatching out of fry, CT cDNAwere amplified as well. We made oral presentation about this result at 71 annual meeting of Zoological Society of Japan in Yamagata, 2000 (Zool. Sci., 17 suppl. 6p). As this result must be the first finding, we will continue to make a research about this phenomenon. Less

  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Urata M, Sasayama Y, Matada M 他: "Calcitonin-immunoreactive cells of the digestive tract of the amphioxus are distributed concentrically in a restricted region of the mid-gut"Acta Zoologica (Stockholum). 82. 73-77 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 笹山雄一: "微小重力下におかれた脊椎動物の生理:特にCa代謝に注目して"宇宙生物科学. 15. 3-14 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sasayama Y, Takemura M, Takano K: "Ultimobranchial glands in the teleost (Plecoglossus attivelis ryukuensis) : Special references to changes of gland volume with matulation"Okajima Tolia Anatomica Japonica. 78. 101-106 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 井尻憲一, 水野りえ, 本田彰, 笹山雄一: "メダカ胚におけるカルシウム代謝の研究-鰓後腺と骨及び耳石研究"Space Utilization Research. 17(印刷中). (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 戒田典久, 笹山雄一: "オニオコゼ3倍体のCa代謝:鰓後腺の形態"平成13年度日本水産学会春季大会講演要旨集. 85 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kobayashi D, Sasayama Y: "Changes in the number of calcitonin-immunoreactive cells in the brain of the brook lamprey during body growth"Zoological Science. 18 suppl.. 24 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sakamoto H, Sasayama Y: "Nucleotide sequence of the CDNA of a bone-mineralizing hormone (calcitonin) in medaka (Teleostai)"The Fish Biol. J. Medaka. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ijiki K, Mizuno R, Honda N: "Sasayama Y Calcium metabolism in medaka embryos - Formation of ultimobranchial gland"cartilage and otoliths. Space Utilization Res.. 17(in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Urata M, Sasayama Y, Matada M, et al: "Calcitonin-immunoreactive cells of the digestive tract of the amphioxus are distributed ncentricaily in a restricted region of the mid-gut"Acta Zool. 82. 73-77 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sasayama Y: "Physiology of vertebrates under micro-gravity with special reference to the Ca metabolism"Biol. Sci. in Space. 15. 3-14 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sasayama Y, Takemura M, Takano K: "Ultimobranchial glands in the teleost (Plecoglossus altivelis ryukyuensis) : Special references to changes of Gland volume with maturation"Okajima Folia Anat. Jap.. 78. 101-106 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sakamoto H, Ueda K, Sasayama Y: "Anuran calcitohins are diverse in lower vertebrates"Zool. Sci.. 17. 97-101 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okuda R, Sasayama Y, Suzuki N, Kamabegawa A: "Srivastav A K Calcitonin cells in the intestine of goldfish and a comparison of the number of cells among saline-fed, soup-fed, or high Ca soup-fed fishes"Gen. Comp. Endocrinol.. 113. 267-273 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzuki N, Ueda K, Sakamaoto H, Sasayama Y: "Fish calcitonin genes : Primitive bony fish genes have been conserved in some lower vertebratres"Gen. Comp. Endocrinol. 113. 369-373 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sasayama Y, Matsubara T, Takano K: "Topography and immunohistochemistry of the ultimobranchial glands in some subtropical fishes"Ichthyol. Res.. 46. 219-222 (1999)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Suzuki N, Suzuki D, Sasayama Y, Srivastav A K, Kmbegawa A, Ashahina K: "Plasma calcium and calcitonin levels in eels fed a high calcium solotion or transferred to seawater"Gen. Comp. Endocrinol.. 114. 324-329

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sasayama Y: "Hormonal control of Ca homeostasis in lower vertebrates : Considering the evolution"Zool. Sci.. 16. 857-869 (1999)

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Published: 2003-09-17  

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