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2000 Fiscal Year Final Research Report Summary

MOLECULAR MECHANISMS OF THE NERVE NET FORMATION IN HYDRA PRIMITIVE NERVOUS SYSTEM

Research Project

Project/Area Number 11640684
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 動物生理・代謝
Research InstitutionFUKUOKA WOMEN'S UNIVERSITY

Principal Investigator

KOIZUMI Osamu  FUKUOKA WOMEN'S UNIVERSITY, ENVIRONMENTAL SCIENCE, PROFESSOR, 人間環境学部, 教授 (50094777)

Co-Investigator(Kenkyū-buntansha) MINOBE Sumiko  FUKUOKA WOMEN'S UNIVERSITY, ENVIRONMENTAL SCIENCE, ASSISTANT, 人間環境学部, 助手 (80190718)
Project Period (FY) 1999 – 2000
KeywordsDiffused nervous system / hydra / nerve net formation / Developmental neurobiology / peptide / Immunohistochemistry / antibody production / nerve differentiation
Research Abstract

(1) Large scale screening of peptide signal molecules : According to our peptide project, the following ideas were clarified. (i) Peptide molecules have developmental functions such as morphogenesis and cell differentiation in addition to the well known function, the transmission of neural signals. (ii) Peptide molecules are epithelial peptides localized in epithelial cells in addition to neuropeptides localized in nerve cells.
(2) Chemical anatomv of the hvdra nervous svstem usinz neuropeptide antibodies : We produced antibodies to all peptides which structures were identified. Using these antibodies, we did chemical anatomy of hydra, and the detailed anatomical chart covering the whole body was able to be made.
(3) Molecular mechanisms of the nerve net formation : We identified peptide molecules controlling the nerve differentiation. Hym355 is a neuropeptide activating the nerve differentiation. LPW peptides are epithelial peptides inhibiting the nerve differentiation.
(4) Nerve cell culture system as a bioassay system. We tried to establish the cell culture system of nerve cells. If it will be established, it will be used as a bioassay system to screen neuroactive substances or active substances influences on the nervous system. The present situation is a success of short term culture of single neurons.

  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] D.Asai: "Preparation of the antibody which discriminates the conformations of ligand-bound and ligand-free estrogen receptors."Peptide Chemistry. (In Press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Takahashi: "Identification of a new member of the LWamide peptide family : Physiological activity and cellular localization of Cnidarian polyps"Biol.Bull.. (In Press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Takahashi: "A novel neuropeptide, Hym 355, positively regulates heuron differentiation in hydra"Development. 127. 997-1005 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Minobe: "Identification and Characterization of the epithelial polarity receptor "Frizzled" in Hydra vulgaris"Development, Genes and Evolution. 210. 258-262 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Hatta: "Identification of a homolog of actin-binding protein, ABP-280, localized at epithelial cell-cell boundaries in hydra"Zoological Science. 16. 439-443 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Inoue : "Localization of the neuropeptide NGIWYamide in the holothurian nervous system and its effects on muscular contraction."Proc.R.Soc.Lond.B. 266. 993-1000 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takahashi, T., Kobayakawa, Y., Muneoka, Y., Fujisawa, Y., Mohri, S., Hatta, M., Shimizu, H., Fujisawa, T., Sugiyama, T., Takahara, M., Yanagi K.and Koizumi, O.: "Identification of a new member of the LWamide peptide family : Physiological activity and cellular localization in Cnidarian polyps."Biol.Bull.. (in Press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Asai, D., Koizumi, O., Shirou Mohri, and Shimohigashi, Y.: "Preparation of the antibody which discriminates the conformations of ligand-bound and ligand-free estrogen receptors : Applications to the quantitative analysis of ligand bindings."Peptide Chemistry. (in Press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takahashi, T, Koizumi, O., Ariura, Y., Romanovitch, A., Bosch, T.C.G., Kobayakawa, Y., Mohri, S., Bode, H., Yum, S., Hatta, M., Fujisawa, T.: "A novel neuropeptide, Hym355, positively regulates neuron differentiation in hydra."Development. 127. 997-1005 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Minobe, S., Fei.K., Yan L., Sarras, M.P.and Werle, M.J.: "Identification and charaterization of the epithelial polarity receptor "Frizzled" in Hydra vulgaris."Dev.Genes Evol.. 210. 258-262 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hatta, T., Sakaguchi, M., Kobayakawa, T., Kishimoto, Y.and Koizumi, O.: "Identification of a homolog of actin-binding protein, ABP-280, localized at epithelial cell-cell boundaries in hydra."Zoological Science. 16. 439-443 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Inoue, M., Birenheide, R., Koizumi, O., Kobayakawa, Y., Mueoka, Y., and Motokawa, T.: "Localization of the neuropeptide NGIWYamide in the holothurian nervous system and its effects on muscular contraction."Proc.R.Soc.Loud.B. 266. 993-1000 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Koizumi, O.: "Neurobiology of the diffused nervous system of hydra.(in Japanese)"Comparative Physioogy and Biochemistry. Vol.16 No.4. 278-287 (1999)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2002-03-26  

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