2000 Fiscal Year Final Research Report Summary
Multi-functional biosensing chip for rapid determination of herbicides for paddy fields
| Project/Area Number |
11650832
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
工業分析化学
|
|---|
| Research Institution | Toyama University (2000)
Kyushu Institute of Technology (1999) |
|---|
Principal Investigator |
SUZUKI Masayasu Toyama University, Faculty of Engineering, Professor, 工学部, 教授 (70226554)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
URANO Kohei Yokohama National University, Faculty of Engineering, Professor, 工学部, 教授 (60018009)
|
|---|
| Project Period (FY) |
1999 – 2000
|
| Keywords | bio-sensing / eco-toxicity / herbicide / chronic toxicity / acute toxicity / green algae / immobilization / chlorophyll |
|---|
| Research Abstract |
This study aims at the basic research for the future development of the integrated biosensing chip for evaluation of acute ecotoxicity indices (inhibition of fluorescence or oxygen evolution) and chronic ecotoxicity index (growth inhibition). In this study, we have investigated the following points : 1)Survey of immobilization method for green algae, 2) Chronic ecotoxicity test by using immobilized green algae, 3) Freezing method of green algae, 4) Quantitative evaluation of inhibitions of fluorescence and oxygen evolution in green algae caused by herbicides, 5) acute toxicity test by using immobilized green algae.
As a green algae immobilization material, photo-crosslinkable prepolymer PVA-SbQ was most suitable. Immobilized green algae showed the similar growth rate to free green algae. By chronic ecotoxicity test using immobilized green algae, most of the herbicides could be detected.
The green algae frozen with 5% dimethyl sulfoxide showed good viability and growth rate after thawing out. Chronic ecotoxicity test also could be done by using this green algae. By the combination of this freezing technique and micro plate assay method, a quite simple novel ecotoxicity test method could be developed.
The inhibition of photosynthetic activity could change chlorophyll-fluorescence intensity and oxygen evolution rate. Ecotoxicity of herbicides was evaluated with fluorescence intensity change and oxygen concentration change. Triazine group herbicides could be successfully determined by this method within an hour.
Finally, a green algae immobilized membrane was set at the sensing area of an oxygen electrode, and atrazine and CAT were determined by using this algae-electrode.
|
