2000 Fiscal Year Final Research Report Summary
The Efferts of UV-A and UV-B radiation on the expression of genes in flower pigment biosynthesis
| Project/Area Number |
11660029
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
園芸・造園学
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| Research Institution | KOBE UNIVERSITY |
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Principal Investigator |
KANECHI Michio Kobe University Faculty of Agriculture, Associate Professor, 農学部, 助教授 (90211854)
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| Co-Investigator(Kenkyū-buntansha) |
UNO Yuichi Kobe University Faculty of Agriculture, Assistant Professor, 農学部, 助手 (90304120)
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| Project Period (FY) |
1999 – 2000
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| Keywords | Anthocyanin / Ultra violet radiation / Flower pigment / Transcription foctor / CHS / Flavonoid / UV |
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| Research Abstract |
The color-changing rose 'Ehigasa' , which petals are redden after flowering under natural daylight, was investigate to clarify the effect of UV light quality (UV-A : 400-320 nm and UV-B : 320-280 nm) on flower pigment (anthocyanin) biosynthesis. Qualification of anthocyanidin skeleton and the bound sugars was investigated by separating of pigments and partial hydrolysates by PC and by measuring absorption spectrum. HPLC analysis of the total anthocyanin content and the ratio of main two anthocyanins to total anthocyanin content at differential developmental stages and light treatment. Main anthocyanins contained in petals of 'Ehigasa' were qualified as cyanidin 3,5-diglucosides (Cy-3G5G) and cyanidin 3-glucoside (Cy-3G). Biosynthesis of anthocyanin stimulated by UV was enhanced with increasing irradiation of visible light at the same time. Tendency of increasing ratio of Cy-3G was observed according to developing flower bud, and the quantity of Cy-3G was exceeded that of Cy-3G5G at unfolded.
Leaf lettuce (cv. Red fire) which has colorful red pigment "anthocyanin" was treated with various UV supplemental irradiations, Chlorophyll and anthocyanin amount was much increased by UV irradiation with all wavelength. UV-B(>290 nm) radiation had no effect on the plant growth and the UV-B(>280 nm) affected leaf growth destructively. Even the low irradiance of UV-B280 was fatal. When the supplemental UV irradiation practiced at night only, the growth and development inhibited largely. While day and night irradiation result in no change in leaf color (the amount of pigments did not increased) but has no effect on growth.
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