2000 Fiscal Year Final Research Report Summary
Design of new pamamycin derivatives that show aerial mycelium-inducing or growth inhibition activity alone
| Project/Area Number |
11660103
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bioproduction chemistry/Bioorganic chemistry
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
NATSUME Masahiro Tokyo University of Agriculture and Technology, Department of Agriculture, Associate Professor, 農学部, 助教授 (10201683)
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| Project Period (FY) |
1999 – 2000
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| Keywords | Streptomyces / Actinomycetes / morphological differentiation / pamamycin / aerial mycelium / biosynthesis / inhibitor |
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| Research Abstract |
1.Isolation, structure elucidation and biological activity of de-N-methylpamamycins A crude pamamycin fraction showed a new Dragendorff reaction-positive spot at Rf 0.30 on TLC analysis. This fraction was purified by silica gel column and ODS- and NH_2-HPLCs giving three new compounds. Their structures were determined as de-N-methylpamamycins by MS spectrometry with the aid of labeled compounds prepared in a methionine-methyl-d_3 feeding culture. De-N-methylpamamycins had stronger aerial mycelium-inducing activity and weaker growth-inhibition activity than the corresponding pamamycin with the same alkyl group arrangement.
2.Search for N,N-didemethylpamamycins To search the presumed biosynthetic precursor, N,N-didemethyl- pamamycin, we cultured Streptomyces alboniger in the presence of methylation inhibitors. As a result, it was found in the extract of DL-ethionine feeding culture. Further investigation on the time of application and dose of ethionine will improve the yield.
3.Isolation of pamamycin homologues with long alkyl side chain Streptomyces sp. HKI-0118 is reported to produce new pamamycin homologues with long alkyl side chain, but their isolation is not achieved. We succeeded in the isolation of these homologues by two NH_2-HPLC columns connected in series.
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