2000 Fiscal Year Final Research Report Summary
The analysis of expressed genes in fish injected with immunostilumant
| Project/Area Number |
11660193
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | Miyazaki University |
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Principal Investigator |
SAKAI Masahiro Miyazaki University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (20178536)
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| Project Period (FY) |
1999 – 2000
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| Keywords | flounder / immunostimulant / gene / CC chemokine / EST |
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| Research Abstract |
Expressed sequence tags (ESTs) were obtained from the kidney of Japanese flounder Paralichthys olivaceus injected with peptidoglycan. The results of single-pass sequencing of ESTs from 198 clones from kidney cDNA will be presented. Sequences of the cDNA clones were compared with sequences in the GenBank database. Eighty and eight clones (44.4 %) appeared to be completely unknown and are likely to represent newly described genes, whereas 110 clones (55.6%) were identified based on matches to sequences in the database. The results contain the genes such as alpha globin, several ribosomal proteins, alpha collagen, heat shock protein 90 and cytochrome oxidase subunit. Immune related cDNAs identified from the kidney were immunoglobulin heavy and light chain, β2-microglobulin, thymosin β and lysozyme, MHC class 11α.
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